In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
Authors Jin J, Shen Y, Zhang B, Deng R, Huang D, Lu T, Sun F, Xu S, Liang C
Received 23 April 2018
Accepted for publication 16 July 2018
Published 12 September 2018 Volume 2018:13 Pages 5321—5333
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Lei Yang
Jing Jin,1 Yanting Shen,2 Biao Zhang,1 Rong Deng,3 Dianshuai Huang,1 Tianqi Lu,1 Fei Sun,1,* Shuping Xu,2,* Chongyang Liang1,*
1Institute of Frontier Medical Science, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 2State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun 130021, Jilin, People’s Republic of China; 3International Joint Research Laboratory of Nano-Micro Architecture Chemistry (NMAC), Jilin University, Changchun 130021, Jilin, People’s Republic of China
*These authors contributed equally to this work
Background: Macropinocytosis can occur in various types of cells and displays multiple functions. However, real-time observation and characterization of the structures of macropinocytosis on the surface of the cell membrane is not yet possible.
Materials and methods: Here, we establish a real-time live cell surface imaging method using three-dimensional-structured illumination microscopy. Based on this, observation of the dynamic macropinocytosis process and morphological data of internalized structures on the surface of pancreatic cancer cells were achieved during macropinocytosis. Next, different-sized silica nanoparticles (SiO2 NPs) were used as the scale for identifying the size range of internalized substances of macropinocytosis in pancreatic cancer cells.
Results and conclusion: Our study not only provides a practical method and more structural data for further investigation of macropinocytosis, but also makes deeper understanding of the cell response toward nanomaterials as well as nanodrugs possible.
Keywords: nanomaterials, macropinocytosis, 3D-SIM, internalization
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]