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Importance Of Laboratory Detection Of Macro-Aspartate Aminotransferase

Authors Ono S, Kurata C, Nishimura N, Kawashima H, Yoneima R, Tai Y, Tatsumi E, Miyamoto M, Yada N, Yoshimoto K, Nishio K

Received 4 August 2019

Accepted for publication 30 October 2019

Published 26 November 2019 Volume 2019:12 Pages 433—436

DOI https://doi.org/10.2147/IJGM.S224281

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Nicola Ludin

Peer reviewer comments 2

Editor who approved publication: Dr Scott Fraser


Shiro Ono,1 Chikara Kurata,2 Nobushiro Nishimura,1 Hiromasa Kawashima,1 Ryo Yoneima,1 Yoshiaki Tai,1 Emiko Tatsumi,1 Makiko Miyamoto,1 Noritaka Yada,1 Kiyomi Yoshimoto,1 Kenji Nishio1

1Department of General Medicine, Nara Medical University, Nara, Japan; 2Department of Clinical Laboratory, Nara Medical University, Nara, Japan

Correspondence: Kenji Nishio
Department of General Medicine, Nara Medical University, 840 Shijo-Cho, Kashihara City, Nara 634-8522, Japan
Tel +81-744-29-8905
Fax +81-744-24-5739
Email knishio@naramed-u.ac.jp

Abstract: The macroenzyme form of aspartate aminotransferase (macro-AST) is formed by the binding of AST with immunoglobulins. Macro-AST excretion from serum is prolonged because of its high molecular weight, leading to increased AST activities. Because of the difficulty in detecting macro-AST through routine laboratory tests, affected patients often undergo repeated examinations, with associated anxiety. We report a case in which macro-AST was detected by assaying the patient’s serum after refrigeration at 4ºC for 3 days. The sample showed progressive loss of AST activity compared with that frozen in the refrigerator, indicating the presence of macro-AST, which was confirmed as a complex with IgG-κ. The cold storage method was validated using many samples obtained from several patients. Use of this simple method to detect macro-AST may avoid unnecessary examinations and patient anxiety even at primary care facilities.

Keywords: isolated elevation of aspartate aminotransferase, macroenzyme, macro-AST, cold storage method

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