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Impact of Ginkgo biloba extract and magnetized water on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rat model

Authors Saleh A, Anwar MM, Zayed AE, Ezz Eldeen MES, Afifi G, Alnashiri HM, Gomaa AMS, Abd-Elkareem M, Abou-Elhamd AS, Shaheen ES, Mohamed GA, Hetta HF, Kotb AM

Received 24 March 2019

Accepted for publication 16 July 2019

Published 7 August 2019 Volume 2019:12 Pages 1339—1347

DOI https://doi.org/10.2147/DMSO.S209856

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Dr Juei-Tang Cheng


Ahmed Saleh,1,2 Mamdouh M Anwar,3,4 Ahmed E Zayed,5,6 Manal El Sayed Ezz Eldeen,7 Gamal Afifi,1,8 Hassien M Alnashiri,5 Asmaa MS Gomaa,4 Mahmoud Abd-Elkareem,6 Alaa Sayed Abou-Elhamd,6,9 Emad S Shaheen,10 Ghada A Mohamed,7 Helal F Hetta,11,12 Ahmed M Kotb6

1Department of Physics, Faculty of Science, Jazan University, Jazan, KSA; 2Exploratory Center of Science and Technology, Cairo, Egypt; 3Department of Pharmacology, Faculty of Pharmacy, Jazan University, Jazan, KSA; 4Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt; 5Department of Biology, Faculty of Science, Jazan University, Jazan, KSA; 6Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Egypt; 7Endocrine Unit, Department of Internal Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt; 8National Institute for Laser Enhanced Sciences, Cairo University, Giza, Egypt; 9Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia; 10Medical Research Centre, Jazan University, Jazan, KSA; 11Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt; 12Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, USA

Introduction: Type 2 diabetes (T2D) is a widely distributed disease that affects large population worldwide. This study aimed to verify the role of Ginkgo biloba (GB) extract and magnetized water (MW) on the survival rate and functional capabilities of pancreatic β-cells in type 2 diabetic rats.
Materials and methods: T2D was induced by feeding the rats on a high-fat diet (20% fat, 45% carbohydrate, 22% protein) for eight weeks followed by intra-peritoneal injection of a single low dose of streptozotocin (25mg/Kg). Forty rats were randomly assigned to four groups (n=10 rats) as follows: non treated control and three diabetic groups. One diabetic group served as a positive control (diabetic), while the other two groups were orally administered with water extract of GB leaves (0.11 g/kg/day) and MW (600 gauss) for four weeks, respectively.
Results: The β-cell mass and insulin expression in these cells increased markedly after both treatments, particularly in GB treated group. In addition, the immune-expression of the two antioxidant enzymes; glutathione and superoxide dismutase 2 (SOD2) in the pancreatic tissue demonstrated a down-regulation in GB and MW treated groups as compared with the diabetic group.
Conclusion: A four-week treatment of GB and MW protected pancreatic β-cell cells and improved their insulin expression and antioxidant status in type 2 diabetic rats.

Keywords: type 2 diabetes, β-cell, Ginkgo biloba extract, magnetized water, glutathione, SOD2

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