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Hydrogen potassium adenosine triphosphatase activity inhibition and downregulation of its expression by bioactive fraction DLBS2411 from Cinnamomum burmannii in gastric parietal cells

Authors Tjandrawinata RR, Nailufar F, Arifin PF

Received 18 June 2013

Accepted for publication 23 July 2013

Published 23 September 2013 Volume 2013:6 Pages 807—815

DOI https://doi.org/10.2147/IJGM.S50134

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3


Raymond R Tjandrawinata1,2, Florensia Nailufar2, Poppy F Arifin1

1Section of Molecular Pharmacology, 2Section of Animal Pharmacology, Dexa Medica, Cikarang, Indonesia

Abstract: This study assessed the gastric acid antisecretory effect of DLBS2411 fractionated from Cinnamomum burmannii. Hydrogen potassium adenosine triphosphatase (H+/K+ ATPase) activity and its gene expression were observed, and the antioxidant activity of DLBS2411 was also investigated. Treatment of DLBS2411 decreased the level of H+/K+ ATPase messenger RNA expression on human embryonic kidney 293 cells and rat gastric parietal cells in a dose-dependent manner, in vitro and ex vivo. DLBS2411 also acted as a competitive inhibitor by showing inhibition in gastric H+/K+ ATPase activity at various pHs. In gastric ulcer animal models induced with indomethacin and ethanol, DLBS2411showed a reduction in the number of petechiae, suggesting that the fraction also confers gastroprotective activity. Moreover, DLBS2411 was also found to have potent antioxidant activity. Taken together, DLBS2411 is a promising novel agent for the management of dyspepsia, a condition of hyperacidity and diseases in the stomach requiring gastroprotection.

Keywords: H+/K+ ATPase, proton-pump inhibitor, proton-pump downregulator, gastroprotective

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