Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17
Authors Ma J, Wei H, Li X, Qu X
Received 14 September 2020
Accepted for publication 19 February 2021
Published 25 March 2021 Volume 2021:13 Pages 2773—2783
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Eileen O'Reilly
Jinhua Ma, Hongbing Wei, Xianlin Li, Xi Qu
Department of Urinary Surgery, The Third People’s Hospital of Hubei Province, Wuhan, Hubei, 430030, People’s Republic of China
Correspondence: Xi Qu
Department of Urinary Surgery, The Third People’s Hospital of Hubei Province, No. 26 Zhongshan Road, Wuhan, Hubei, 430030, People’s Republic of China
Tel +86 13476046068
Email [email protected]
Background: MicroRNAs (miRNAs) are key players in the progression of human cancers. While several miRNAs have been reported to regulate the development of tumors, the molecular mechanisms and roles of miR-149-5p in prostate carcinoma (PCa) remain unclear. Our aim was to investigate the interaction and functions of miR-149-5p and RGS17 in PCa.
Methods: Microarray analysis was performed to identify the key miRNA and gene involved in PCa progression. The expression levels of miRNA and mRNA in PCa tissues and cells were verified by qRT-PCR. MTT assay, BrdU proliferation assay and wound-healing assay were applied to assess the effect of miR-149-5p and RGS17 on PCa cells’ viability, proliferation, and migration ability. The association between RGS17 and miR-149-5p was identify using dual-luciferase reporter assay and Western blot assay.
Results: Data analysis indicated the reduction of miR-149-5p expression in PCa tissues and cells. Experimental investigations also showed that this miRNA suppressed the viability, proliferation and migration ability of PCa cells. RGS17 was found to be the target of miR-149-5p, and the low expression of miR-149-5p upregulated RGS17 in PCa tissues and cells. The results of the cell-function assays showed that RGS17 acted as an oncogene in PCa even though its promotive effect could be reversed by miR-149-5p.
Conclusion: This research confirmed that by targeting and inhibiting RGS17, miR-149-5p could suppress PCa development.
Keywords: miR-149-5p, prostate carcinoma, PCa, RGS17, malignancy
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