HPLC-NNE13CNMR coupling fingerprint analysis technology and its application in a study of Syringa pubescens Turcz and its activity against hepatic fibrosis
Zhixin Zhang,1 Weiping Yin,1 Pu Liu,1 Tianzeng Zhao2
1School of Chemical Engineering and Pharmaceutics, Henan University of Science and Technology, Luoyang, People’s Republic of China; 2Key Laboratory of Natural Products, Henan Academy of Sciences, Zhengzhou, People's Republic of China
Abstract: This study describes the active ingredients of Syringa pubescens Turcz which has been identified as being able to protect against hepatic fibrosis. Here we report the characteristics of high performance liquid chromatography and non-nuclear overhauser effect carbon-13 nuclear magnetic resonance (HPLC-NNE13CNMR) technology developed for coupling fingerprint analysis. The major contribution of this new method is the development of an efficient technology and a useful tool for analysis of a traditional Chinese herbal medicine using chromatography and spectral coupling fingerprint technology. Isolation of secoiridoid glycosides and investigation of their structure-activity relationship showed that these derivatives are the active ingredients of Syringa pubescens Turcz, and account for the activity of this plant against hepatic fibrosis. The active compounds were identified as oleuropein, 10-hydroxyoleuropein, oleoside-11-methylester, (8Z)-ligstroside, and echinacoside by HPLC-NNE13CNMR coupling fingerprint analysis. A concentration-response relationship was also demonstrated for the HPLC-NNE13CNMR coupling fingerprint method.
Keywords: HPLC-NNE13CNMR, coupling fingerprint, hepatic fibrosis, Syringa pubescens Turcz, analysis technology
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