BCL-2 Inhibitor Venetoclax Induces Autophagy-Associated Cell Death, Cell Cycle Arrest, and Apoptosis in Human Breast Cancer Cells

Introduction Venetoclax (VCX) is a selective BCL-2 inhibitor approved for the treatment of leukemia and lymphoma. However, the mechanisms of anti-cancer effect of VCX either as a monotherapy or in combination with other chemotherapeutic agents against breast cancer need investigation. Methods Breast cancer cell lines with different molecular subtypes (MDA-MB-231, MCF-7, and SKBR-3) were treated with different concentrations of VCX for indicated time points. The expression of cell proliferative, apoptotic, and autophagy genes was determined by qRT-PCR and Western blot analyses. In addition, the percentage of MDA-MB-231 cells underwent apoptosis, expressed higher oxidative stress levels, and the changes in the cell cycle phases were determined by flow cytometry. Results Treatment of human breast cancer cells with increasing concentrations of VCX caused a significant decrease in cells growth and proliferation. This effect was associated with a significant increase in the percentage of apoptotic MDA-MB-231 cells and in the expression of the apoptotic genes, caspase 3, caspase 7, and BAX, with inhibition of anti-apoptotic gene, BCL-2 levels. Induction of apoptosis by VCX treatment induced cell cycle arrest at G0/G1 phase with inhibition of cell proliferator genes, cyclin D1 and E2F1. Furthermore, VCX treatment increased the formation of reactive oxygen species and the expression level of autophagy markers, Beclin 1 and LC3-II. Importantly, these cellular changes by VCX increased the chemo-sensitivity of MDA-MB-231 cells to doxorubicin. Discussion The present study explores the molecular mechanisms of VCX-mediated inhibitory effects on the growth and proliferation of TNBC MDA-MB-231 cells through the induction of apoptosis, cell cycle arrest, and autophagy. The study also explores the role of BCL-2 as a novel targeted therapy for breast cancer.


Introduction
Breast cancer (BC) is one of the highest leading causes of cancer deaths. 1,2BC affects one in eight women and almost one million new cases of BC are identified every year worldwide. 3Thus, BC causes serious public health problems owing to its complexity, incidence, prevalence, mortality, heterogeneity, and, ultimately, the economic impact of therapy.The primary biomarkers for BC are the estrogen

Materials and Methods Chemicals
Venetoclax and doxorubicin were obtained from LC laboratories (Woburn, MA, USA).Bovine serum albumin and protease cocktail inhibitor reagents were purchased from Sigma Chemical Co.(St.Louis, MO, USA).High-Capacity cDNA Reverse Transcription kit and SYBR ® Green PCR Master Mix were obtained from Applied Biosystems ® (Foster City, CA, USA).Primary antibodies to target proteins and peroxidaseconjugated antibodies were purchased from Santa Cruz Biotechnology, Inc.

Cell Lines Culture and Treatment
Human breast cancer cell lines, MCF-7 (ER+), SKBR-3 (HER2+), and MDA-MB-231 (TNBC), were obtained from the American Type Culture Collection (Rockville, MD, USA).The cells were grown in Dulbecco's Modified Eagle Medium with phenol red supplemented with Fetal Bovine Serum (FBS, 10%) and Antibiotic-Antimycotic (1%) using standard cell culture methods.Venetoclax and doxorubicin were prepared fresh in dimethyl sulfoxide (DMSO) before each experiment.The same concentration of DMSO was used as a control.Briefly, cells were seeded on 6-or 12-well culture plates to reach 80% confluency, after which they were treated for 24 h with DMSO or VCX (10, 25, and 50 μM), unless otherwise specified.

Apoptosis Assay
The number and percentage of cells undergoing apoptosis were determined using Muse ® Annexin V & Dead Cell Kit, EMD Millipore Co. (Billerica, MA, USA) according to the manufacturer's instructions and as described previously. 14Briefly, 80% confluent MDA-MB-231 cells were incubated for 24 h with VCX (10, 25, and 50 µM).The cells were collected by trypsinization, centrifuged at 300×g for 5 min, and then resuspended in 100 µL 1% FBS followed by incubation with Muse ® Annexin V & Dead Cell reagent in the dark.The percentage of healthy, apoptotic, and dead cells was counted with the Muse ® Cell Analyzer, EMD Millipore Co. (Billerica, MA, USA).

Caspase 3/7 Activities
The quantitative measurements of apoptotic status based on caspase 3/7 activation were examined using Muse ® Caspase 3/7 Activation Kit, EMD Millipore Co. (Billerica, MA, USA), as described previously. 15Briefly, after 24 h treatment with VCX, cells were trypsinized, centrifuged at 300×g for 5 min, and then resuspended in 1X Assay Buffer, followed by incubation with 5 µL of caspase 3/7 reagent working solution for 30 min at 37°C in 5% of CO 2 incubator.After incubation, 150 µL of Muse ® Caspase 7-ADD working solution was added and incubated at room temperature for 5 min in the dark.The percentage of caspase 3/7 positive cells was measured using Muse ® Cell Analyzer, EMD Millipore Co. (Billerica, MA, USA).

Protein Extraction and Western Blot Analysis
Total protein isolated from MDA-MB-231 cells was quantified by Infrared (IR) spectroscopy Direct Detect ® , EMD Millipore Co. (Billerica, MA, USA). 24Western blot analysis was performed as described before 24 by separating approximately 30 μg protein in 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and then transferred to a nitrocellulose membrane, Bio-Rad (Mississauga, ON, Canada).The protein blots were blocked overnight at 4°C, followed by incubation, at 4°C, with primary antibodies against target proteins followed by incubation with appropriate peroxidaseconjugated secondary antibody for 2 h at room temperature.The bands were visualized by chemiluminescence kits, EMD Millipore Co. (Billerica, MA, USA) using C-DiGit ® Blot Scanner, LI-COR Biosciences (Lincoln, NE, USA).

Cell Cycle Distribution and Progression Analysis
Cell cycle distribution and progression were analyzed using Muse ® Cell Cycle Kit, EMD Millipore Co. (Billerica, MA, USA). 14Briefly, cells treated with VCX for 24 h were collected by trypsinization, centrifuged at 300×g for 5 min, and then resuspended in phosphatebuffered saline followed by incubation with 70% cold ethanol at −20°C for at least 30 min prior to staining with a premixed reagent propidium iodide (PI) and RNAse A. The DNA contents at all cell cycle stages were quantified using Muse ® Cell Analyzer, EMD Millipore Co. (Billerica, MA, USA).

Reactive Oxygen Species (ROS) Production Assay
The cellular population undergoing oxidative stress was detected by measuring the levels of reactive oxygen species (ROS) using Muse ® Oxidative Stress Kit, EMD Millipore Co. (Billerica, MA, USA), as described previously 25 and according to the manufacturer's instructions.Briefly, pelleted cells treated with VCX were stained with Muse ® Oxidative Stress Working Solution that contains dihydroethidium (DHE), which is permeable and can react with superoxide anions undergoes oxidation to form the DNA-binding fluorophore ethidium bromide.The fluorescence produced was measured using Muse ® Cell Analyzer, EMD Millipore Co. (Billerica, MA, USA).

Statistical Analysis
The statistical analysis of the results was performed using Sigma Stat ® for Windows, Systat Software Inc. (San Jose, CA, USA).Student's t-test or one-way analysis of variance (ANOVA) followed by Student-Newman-Keul's (SNK) tests were performed and the differences were considered significant when p <0.05.

Effect of VCX Treatment on the Proliferation and Growth of Different Types of Breast Cancer Cell Lines
We first assessed the ability of VCX to inhibit the growth and proliferation of three breast cancer cell lines of different molecular subtypes, SKBR-3 (HER2+), MCF-7 (ER+), and MDA-MB-231 (TNBC).The cells were incubated for 24 h with wide range of VCX concentrations (0.5, 1, 2.5, 5, 10, 25, 50, and 100 μM), those concretions were selected based on previous studies, 10,13 after that cell proliferation was determined as described in the Materials and Methods section.Figure 1A shows that VCX at concentrations up to 5 μM did not significantly alter cell viability and proliferation of all tested cells.However, a significant decrease in cell proliferation was observed at VCX concentrations 10, 25, 50, and 100 μM in all tested cells in a concentration-dependent manner (Figure 1A).The IC 50 of VCX for MDA-MB-231 was approximately 60 ± 4.2 μM, whereas MCF-7 and SKBR-3 cells were more sensitive to VCX inhibitory effects with IC 50 of 36 ± 5.3 and 34 ± 7.1 μM, respectively (Figure 1A).Based on cell viability/ proliferation studies, the concentrations 10, 25, and 50 μM have been selected to be utilized in all subsequent studies using TNBC MDA-MB-231 cell line as a study model.

Effect of VCX Treatment on Cell Mitochondrial Potential and Cellular Plasma Membrane Permeabilization
Depolarization of the mitochondrial membrane potential prevents calcium entry into the mitochondria causing cell Beclin1 TGAGGGATGGAAGGGTCTAAG GCCTGGGCTGTGGTAAGTAATC [23]   submit your manuscript | www.dovepress.com

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OncoTargets and Therapy 2020:13  viability reduction, and this is considered as an indicator for early apoptosis.To test whether the inhibitory effect of VCX on MDA-MB-231 cell proliferation is due to the depolarization of the mitochondria membrane, we examined the effect of VCX on the mitochondrial potential and cellular plasma membrane permeabilization, a marker for cell death. 26For this purpose, MDA-MB-231 cells were treated for 24 h with VCX (10, 25, and 50 μM), and then the percentage of live cells with intact mitochondria, depolarized live and dead cells, and dead cells with intact mitochondria were determined by flow cytometry.Figure 1B shows that VCX treatment at 10 μM concentration did not significantly alter the mitopotential and membrane permeability.However, higher VCX concentrations (25 and 50 μM) significantly increased the percentage of depolarized (live and dead) cells up to 27% and 48%, respectively, as compared to the control (8%).In addition, the percentage of live cells was significantly decreased to 38% at the highest concertation tested (50 μM) (Figure 1B).

Effect of VCX Treatment on Apoptosis
To explore the mechanisms of the VCX inhibitory effect on MDA-MB-231 cell growth and proliferation, we investigated whether this effect could be attributed to increased apoptotic and/or necrotic cell population.Treatment of MDA-MB-231 cells for 24 h with VCX (10, 25, and 50 μM) significantly increased the percentages of apoptotic cells (early and late) at all tested concentrations, in a concentration-dependent manner, by approximately 2-, 4-, and 6-fold, respectively, as compared to the control (Figure 2).
To further examine whether increased the percentage of apoptotic cells by VCX treatment is associated with changes in the activity and expression of pro-apoptotic and anti-apoptotic markers, we measured the effect of 24 h treatment of VCX on the expression of caspases 3/7, BAX, and BCL-2 in MDA-MB-231 cells.Our results show that VCX 25 and 50 μM treatment significantly increased caspases 3/7 activity using flow cytometry (Figure 3A) and   3B) levels in a concentration-dependent manner.At the protein level, VCX treatment induced the proapoptotic caspase 3 and BAX proteins by approximately 6-and 5-fold, respectively, whereas dramatically inhibited the anti-apoptotic BCL-2 protein by more than 65% at the highest concentrations tested, 50 μM (Figure 3C).The BAX: BCL-2 ratio was increased by VCX in a concentrationdependent manner reached up to 7-and 14-folds at concentrations 25 and 50 μM, respectively (Figure 3D).

Effect of VCX Treatment on Cell Cycle Phases and Genes
To examine whether the arrest of the cell cycle is contributing to the inhibitory effect of VCX on MDA-MB-231 cell proliferation and growth, we performed a cell cycle analysis by flow cytometry.Figure 4A  significantly increased the percentage of cell population in the G0/G1 phase to 65% (50% increase) as compared to control (43%), whereas decreased cell population in the S phase to 16% (50% decrease) as compared to control (34%).No significant changes in G2/M phase were observed in response to VCX treatment (Figure 4A).
To further determine whether the cell cycle arrest by VCX is attributed to a downregulation in the expression of cell cycle proliferative genes, we quantified the mRNA and protein expression levels of cyclin D1 and E2F1, well-known cell cycle genes, by qRT-PCR and Western blot analyses, respectively.Data in Figure 4B illustrate that VCX significantly inhibited cyclin D1 and E2F1 mRNA expression by approximately 55% and 70%, respectively, at VCX 50 μM concentration.At the protein expression level, cyclin D1 and E2F1 proteins were markedly inhibited by all tested VCX concentrations, reached up to 90% by VCX 50 μM as compared to control (Figure 4C).

Effects of VCX Treatment on Oxidative Stress
We tested the role of oxidative stress in VCX inhibitory effect on cell proliferation by first measuring the percentage of MDA-MB-231 cells generating ROS and second by quantifying the mRNA and protein expression levels of oxidative stress-mediated genes, HO-1 and GSTA.The flow cytometry analysis data in Figure 5A show that VCX 25 and 50 μM treatment significantly increased the percentage of cells generating ROS to 9% and 11%, respectively, as compared to 5% in control cells.The increased ROS formation was associated with a concentration-dependent induction of HO-1 and GSTA mRNA (Figure 5B) and protein (Figure 5C) expression levels.

Effect of VCX on Cell Autophagy
The possibility that VCX induces cell apoptosis via an autophagy-mediated cell death was tested by measuring the mRNA and protein expression of well-known markers of cell autophagy (LC3 and Beclin1) 27 in response to VCX 25 μM. Figure 6A shows that VCX induced autophagy-associated cell death through increasing the mRNA expression of LC3 and beclin1 by approximately 30% and 40%, respectively.This was confirmed at the protein expression level, where VCX increased the expression of LC3II protein level by 3-fold (Figure 6B).

Effect of VCX on the Chemo-Sensitivity of MDA-MB-231 Cells to DOX
To test whether VCX would increase the chemosensitivity of MDA-MB-231 cells to chemotherapy, we examined the effect of VCX treatment in combination with DOX on MDA-MB-231 apoptosis level as a marker for chemo-sensitivity.Cells were treated for 24 h with VCX (25 μM) in the presence and absence of DOX (100 nM).Then, the percentage of cells that underwent apoptosis/necrosis was determined by flow cytometry.VCX or DOX monotherapy increased the percentage of apoptotic cells to 23% and 20%, respectively, as compared to control (8%).Importantly, DOX and VCX combination therapy synergistically increased the percentage of apoptotic cells to 71% as compared to DOX alone (Figure 7).

Discussion
The predominant role of BCL-2 family is to regulate cell survival by modulating the integrity and the release of apoptogenic factors. 28Not surprisingly, BCL-2 overexpression has been linked to tumorigenesis initiation and the development of resistance to chemotherapies. 29ue to their regulatory roles in apoptosis, members of the BCL-2 family are considered as promising targets for cancer therapy. 28,29VCX is a potent and selective BCL-2 inhibitor with anti-tumor properties that usually correlates with its inhibitory effect on BCL-2.With increased BCL-2 expression in BC and particularly in TNBC, the possibility that VCX may suppress TNBC proliferation could not be ruled out.The available studies on the effect of VCX on BC cells proliferations only investigated the role of apoptosis, whereas the involvement of other mechanisms such as autophagy, cell cycle arrest, and oxidative stress has not been investigated.Therefore, the main objective of the current study was to explore the mechanistic role of autophagy, cell cycle arrest, and oxidative stress in VCXinduced breast cancer cell proliferation and growth inhibition.
The present study demonstrates the ability of VCX to cause a concentration-dependent cell growth inhibition in three breast cancer cell lines that pose different molecular subtypes, MCF-7 (ER+), SKBR-3 (HER-2+), and MDA-MB-231 (TNBC).Cell growth inhibition of VCX was more effective on both MCF-7 and SKBR-3 cells than on MDA-MB-231, with IC 50 of approximately 35 μM for MCF-7 and SKBR-3 and 60 μM for MDA-MB-231 cells.The differential sensitivity of the cells to VCX could be attributed to several factors including the expression level of BCL-2.For example, BCL-2 is overexpressed in 85% of cases of luminal cancers (ER+), 30 whereas overexpressed in approximately 41% of TNBC cases, 5 which could explain the least sensitivity of MDA-MB-231 cells to VCX.The in vitro concentrations of VCX used in this study were maintained close to the therapeutic range of plasma concentration reported in humans. 31However, using higher VCX concentrations in the current study is attributed to the excessive concentrations of glucose, FBS, growth factors which all stimulate cell growth to promote breast cancer aggression and aggressiveness of TNBC. 32ther possible factors include the presence of an oncogenic mutation in the cell culture system, which reduces the efficacy of VCX concentrations required to achieve the anti-cancer effects.In MDA-MB-231 cells, the ability of VCX treatment to induce mitochondrial dysfunction and membrane permeabilization is substantial evidence supporting VCX-induced apoptosis in a mitochondriamediated pathway.The possibility that VCX exhibits anti-proliferative effects on MDA-MB-231 cells through induction of apoptosis was supported by the ability of VCX to a) increase the percentage of MDA-MB-231 cells undergo apoptosis and induce caspase 3/ 7 activity and gene expression at the mRNA and protein levels and b) induce the expression of several proapoptotic markers, caspase 3, caspase 7, BAX, while inhibit the expression of the anti-apoptotic BCL-2 with increased BAX/BCL-2 ratio.Our findings are in an agreement with previous observations, which showed that VCX induces apoptosis 10 in breast cancer cells through BAX-dependent mechanism. 33,34Increased cell apoptosis by VCX in the current study was associated with induction of cell cycle arrest in G0/G1, leading to an increase in the percentage of cells in the G0/G1 phase with a decrease in the S phase cell population.The probable mechanism mediating the cell cycle arrest by VCX is the downregulation of positive regulators of G1-to-S transition including cyclin D1  though the inhibition of E2F1 transcription factor that is known to regulate cyclin D1 promotor. 35Although the exact mechanism of downregulation of cyclin D1 by VCX was not explored in this study, increased cyclin D1 degradation post-translationally by ubiquitination or phosphorylation is postulated, 36 however further studies are needed.These findings agree with earlier studies showing that overexpression of cyclin D1 gene was observed steadily in breast tumors. 37,38In addition, it has been proposed that cellular senescence, which is a permanent cell cycle arrest in G0/G1 in response to different stressors, contributes to tumor supression. 39,40nother possible mechanism that could regulate VCX-induced apoptosis is oxidative stress. 41In this context, we report here that VCX-induced apoptosis was proportionally associated with a significant increase in ROS production and the expression of oxidative stress marker genes, HO-1 and GSTA, at both the mRNA and protein levels.Despite the fact that BCL-2 overexpression promotes cancer cell survival, recent reports provide evidence that the BCL-2 family also involves a redox component. 42Previous observations support our current results in which these studies showed that inhibition of cancer cells proliferation and induction of apoptosis and cell cycle arrest are mediated by induction of oxidative stress genes probably via the activation of ubiquitindependent proteasome degradation of cyclin D1. 43 Importantly, induction of oxidative stress by VCX is believed to mediate the activation of autophagyassociated cell death pathway markers, LC3II and Beclin1, at the mRNA and protein levels.To our knowledge, this is the first report to demonstrate the inter-relationship between induction of apoptosis and autophagy-associated cell death by VCX-mediated inhibition of BCL-2 in MDA-MB-231 cells.In this regard, it was reported that Beclin1, an essential initiator of autophagy pathway, has a BCL-2 homology (BH3) domain.Interaction of BCL-2 protein with Beclin1 BH3 domain prevents assembling of the pre-autophagosome structure leading to autophagy inhibition, indicating that downregulation of BCL-2 by VCX is the first step toward activation of autophagy-associated cell death pathway. 44he dual induction of apoptosis and autophagicassociated cell death makes VCX an ideal chemotherapeutic target for breast cancer.

ROS
DOX and other anthracyclines trigger a DNA damage response and subsequently activate an apoptotic pathway to kill cancer cells. 45,46Since BCL-2 family plays a significant role in regulating apoptotic cell death, it is possible that inhibition of BCL-2 restores sensitivity to chemotherapeutic agents.The present findings show that the combination of VCX with DOX increases the chemosensitivity of MDA-MB-231 to DOX, and this could provide a new strategy to overcome the protective effects of BCL-2 and hence deliver efficacious therapies.These findings also suggest the potential of VCX to be used clinically to increase the efficacy of DOX and other chemotherapeutic agents.

Conclusions
The present study demonstrates strong evidence that VCX a) induces human triple-negative breast cancer MDA-MB -231 cell growth inhibition through apoptosis, cell cycle arrest, autophagy-associated cell death, and oxidative stress mechanisms and b) increases the chemosensitivity to DOX in MDA-MB-231 cells.

Figure 1
Figure 1 Effect of VCX on the proliferation and growth of human breast cancer cells.(A) Human breast cancer cell lines; SKBR-3, MCF-7, and MDA-MB-231 cells were treated for 24 h with a wide range of VCX concentrations.Cell proliferation and viability were determined by Muse ® Count & Viability assay.(B) MDA-MB -231 cells were treated with VCX (10, 25, and 50 μM) for 24 h.Thereafter, the percentages of live, depolarized, and dead cells were determined by Muse ® MitoPotential Kit using Muse ® Cell Analysis.The histogram represents the mean, (n = 3, triplicate).*P < 0.05 compared to control, VCX=0 μM, (ANOVA followed by SNK test).

Figure 4
Figure 4 Effect of VCX treatment on MDA-MB-231 cell cycle phases and genes.MDA-MB-231 cells were treated for 24 h with various concentration of VCX (10, 25, and 50 μM).(A) Cell cycle phases were determined by Muse ® Cell Cycle assay.Values are presented as the mean of percentage of live cells (n = 3, triplicate).(B) Cyclin D1 and E2F1 mRNA levels were quantified using qRT-PCR and normalized to β-actin housekeeping gene.Triplicate reactions were performed for each experiment.The values represented the mean of fold change ± SEM, (n = 6).*P < 0.05 compared to control, VCX=0 μM, (ANOVA followed by SNK test).(C) Cyclin D1 and E2F1 protein expression levels were determined by Western blot analysis.One of three representative experiments is shown.The values represented the mean of fold change ± SEM, (n = 3).*P < 0.05 compared to control, VCX=0 μM, (ANOVA followed by SNK test).

Figure 6
Figure 6 Effect of treatment on autophagy in MDA-MB-231cells.MDA-MB -231 cells were treated for 24 h with VCX (25 μM).(A) LC3 and Beclin1 mRNA levels were quantified using qRT-PCR and normalized to β-actin housekeeping gene.The values are presented as mean ± SEM, (n = 6, triplicate).*P < 0.05 compared to control, VCX=0 μM, (Student's t-test).(B) LC3I and II protein levels were determined by Western blot analysis.One of three representative experiments is shown.The values are presented as mean ± SEM. *p< 0.05 compared to control, VCX=0 μM, (Student's t-test).

Figure 7
Figure 7 Effect of VCX on the chemo-sensitivity of MDA-MB-231 cells to DOX.MDA-MB-231 cells were treated for 24 h with VCX 25 μM alone or in combination with DOX 100 nM.Thereafter, the percentage of cell underwent apoptosis were determined using Muse ® Annexin V & Dead Cell Kit.The values are presented as mean, (n = 3, triplicate).*P < 0.05 compared to control, VCX=0 μM, # P < 0.05 compared to DOX, (ANOVA followed by SNK test).

Table 1
Primers Sequences Used for qRT-PCR Reactions