Genetic Polymorphisms in Transforming Growth Factor-β, Interferon-γ and Interleukin-6 Genes and Susceptibility to Behcet’s Disease in Saudi Population
Authors Al Okaily F, Alrashidi S, Mustafa M, Alrashdan FB
Received 13 February 2020
Accepted for publication 28 May 2020
Published 31 July 2020 Volume 2020:13 Pages 253—259
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Martin H Bluth
Fahdah Al Okaily,1 Seham Alrashidi,1 Mohammad Mustafa,2 Faris Bander Alrashdan3
1Department of Rheumatology, Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia; 2Scientific Research Center, Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia; 3Department of Internal Medicine, Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia
Correspondence: Fahdah Al Okaily
Department of Rheumatology, Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia
Background: Behcet’s disease (BD) is a complex, inflammatory, immune-mediated multi-systemic disease of unknown etiology. Cytokines play major roles in the pathophysiology of BD, and its production may be affected by polymorphism in cytokine genes. Hence, the present study was planned to investigate any possible association between the polymorphism in TGF-β, IFN-γ, and IL-6 genes and BD in the Saudi population.
Materials and Methods: The present study includes 79 BD cases and 117 age-matched controls. Polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation systems (ARMS) PCR methods were used for polymorphic analysis.
Results: The heterozygous (CT) and (CT+TT) combined genotypes of TGF-β (509-C/T), heterozygous (AT), variant (TT), and (AT+TT) combined genotypes of IFN-γ (874-A/T) were significantly (P< 0.05) associated with BD in the Saudi population. No significant differences were observed for IL-6 (174-G/C) genotypes and alleles between BD cases and controls. Gender does not reflect any significant genotypic and allelic association with males and females.
Conclusion: CT genotype of TGF-β, and AT and TT genotypes of IFN-γ could be associative genetic risk factors for BD in Saudis. Regulatory region polymorphism in cytokines gene can increase inflammation and deregulated immune response, which could be risk factor for BD.
Keywords: tumor growth factor-β, Interferon-γ, Interleukin-6, Behcet’s disease, Saudis, genetic polymorphism
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