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GATA3 is downregulated in osteosarcoma and facilitates EMT as well as migration through regulation of slug

Authors Ma L, Xue W, Ma X

Received 7 June 2018

Accepted for publication 6 August 2018

Published 30 October 2018 Volume 2018:11 Pages 7579—7589

DOI https://doi.org/10.2147/OTT.S176534

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Dr Leo Jen-Liang Su


Linjie Ma,1 Wentao Xue,1 Xianghai Ma2

1Department of Orthopedics, Yidu Central Hospital of Weifang City, Qingzhou 262500, People’s Republic of China; 2Department of Orthopedics, People’s Hospital of Juxian, Juxian, Shandong 276500, People’s Republic of China

Background: GATA3 functions as a tumor suppressor and has been observed in multiple types of cancer, but the effects and mechanisms of GATA3 in osteosarcoma (OS) are not yet known.
Methods: The GATA3 expression in OS cells and tissues were detected using quantitative reverse-transcription PCR and Western blotting assay. CCK-8 assay, colony formation assay, wound healing assay as well as transwell assay, were performed to determine the effects of GATA3 on cell proliferation, migration and invasion. ChIP and qChIP as well as luciferase assay were performed whether GATA3 transcriptionally regulated slug expression.
Results: GATA3 was downregulated in OS cells and tissues. The GATA3 expression was closely associated with tumor size as well as metastasis. GATA3 significantly suppressed OS cells proliferation, migration and invasion. EMT-associated transcript factor, slug, was transcriptionally inhibited by GATA3, thereby regulation of EMT in OS.
Conclusion: GATA3 serves as a tumor suppressor in OS and suppresses the progression and metastasis of OS through regulation of slug.

Keywords: GATA3, osteosarcoma, epithelial–mesenchymal transition, proliferation, migration
 

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