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GATA3 is downregulated in osteosarcoma and facilitates EMT as well as migration through regulation of slug
Authors Ma L, Xue W, Ma X
Received 7 June 2018
Accepted for publication 6 August 2018
Published 30 October 2018 Volume 2018:11 Pages 7579—7589
DOI https://doi.org/10.2147/OTT.S176534
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Amy Norman
Peer reviewer comments 3
Editor who approved publication: Dr Leo Jen-Liang Su
Linjie Ma,1 Wentao Xue,1 Xianghai Ma2
1Department of Orthopedics, Yidu Central Hospital of Weifang City, Qingzhou 262500, People’s Republic of China; 2Department of Orthopedics, People’s Hospital of Juxian, Juxian, Shandong 276500, People’s Republic of China
Background: GATA3 functions as a tumor suppressor and has been observed in multiple types of cancer, but the effects and mechanisms of GATA3 in osteosarcoma (OS) are not yet known.
Methods: The GATA3 expression in OS cells and tissues were detected using quantitative reverse-transcription PCR and Western blotting assay. CCK-8 assay, colony formation assay, wound healing assay as well as transwell assay, were performed to determine the effects of GATA3 on cell proliferation, migration and invasion. ChIP and qChIP as well as luciferase assay were performed whether GATA3 transcriptionally regulated slug expression.
Results: GATA3 was downregulated in OS cells and tissues. The GATA3 expression was closely associated with tumor size as well as metastasis. GATA3 significantly suppressed OS cells proliferation, migration and invasion. EMT-associated transcript factor, slug, was transcriptionally inhibited by GATA3, thereby regulation of EMT in OS.
Conclusion: GATA3 serves as a tumor suppressor in OS and suppresses the progression and metastasis of OS through regulation of slug.
Keywords: GATA3, osteosarcoma, epithelial–mesenchymal transition, proliferation, migration
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