GALE Promotes the Proliferation and Migration of Glioblastoma Cells and Is Regulated by miR-let-7i-5p
Received 2 July 2019
Accepted for publication 16 November 2019
Published 16 December 2019 Volume 2019:11 Pages 10539—10554
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Professor Bilikere Dwarakanath
Xiaopeng Sun,1,2 Hao Xue,1,3 Ye Xiong,4 Rui Yu,5 Xiao Gao,1 Mingyu Qian,1 Shaobo Wang,1 Huizhi Wang,1 Jianye Xu,1 Zihang Chen,1 Lin Deng,1,3 Gang Li1,3
1Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province 250012, People’s Republic of China; 2Department of Neurosurgery, Dezhou People’s Hospital, Dezhou, Shandong Province 253014, People’s Republic of China; 3Institute of Brain and Brain-Inspired Science, Shandong Provincial Key Laboratory of Brain Function Remodeling, Shandong University, Jinan, Shandong Province, People’s Republic of China; 4Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, People’s Republic of China; 5Department of Neurosurgery, The Second Hospital of Shandong University, Jinan 250033, People’s Republic of China
Correspondence: Gang Li; Lin Deng
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province 250012, People’s Republic of China
Email firstname.lastname@example.org; email@example.com
Purpose: Glioma is the most common and lethal type of brain tumor. While GALE (UDP-galactose-4-epimerase) has been shown to be overexpressed in some kinds of cancers, its expression in gliomas has not been reported. MicroRNAs (miRNAs) function as tumor suppressors in many cancers, and online datasets can be used to predict whether GALE is regulated by miR-let-7i-5p. In this investigation, we explored the effect and regulatory mechanisms of GALE on glioblastoma growth via miR-let-7i-5p.
Methods: We used a Cox proportional hazards model and publicly available datasets to examine the relationship between GALE and the survival rates of glioma patients. Bioinformatics predicted the targeting of GALE by miR-let-7i-5p. The proliferation, migration, cell cycle and apoptosis of human glioblastoma cells were assessed by relevant assays. We also demonstrated the effect of GALE on glioblastoma multiforme [GBM] tumor growth using an in vivo orthotopic xenograft model.
Results: GALE was upregulated in human gliomas, especially in high-grade gliomas (e.g., GBM). An obvious decline in GALE expression was observed in human glioblastoma cell lines (U87 and U251) following treatment with a small interfering RNA (siRNA) targeting GALE or miR-let-7i-5p mimics. Knockdown of GALE or overexpression of miR-let-7i-5p (with miR-let-7i-5p mimics) inhibited U87 and U251 cell growth. miR-let-7i-5p significantly restrained the migration ability of human glioblastoma cells in vascular mimic (VM), wound healing and transwell assays, and GALE promoted glioblastoma growth in vivo.
Conclusion: Our findings confirm that GALE plays an important role in promoting the development of human glioma and that GALE can be regulated by miR-let-7i-5p to inhibit human glioblastoma growth.
Implications for cancer survivors: Our data show that cancer survivors have low GALE expression, which indicates that GALE may be a diagnostic biomarker and a promising therapeutic target in glioblastoma.
Keywords: glioblastoma, GALE, miR-let-7i-5p, proliferation, migration
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