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Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Authors Veerapandian M, Seo, Shin, Yun K, Lee M

Received 21 September 2012

Accepted for publication 27 October 2012

Published 18 December 2012 Volume 2012:7 Pages 6123—6136

DOI https://doi.org/10.2147/IJN.S38402

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Murugan Veerapandian,1 Yeong-Tai Seo,2 Hyunkyung Shin,3 Kyusik Yun,1 Min-Ho Lee4

1Department of Bionanotechnology, Gachon University, Gyeonggi-Do, 2School of Electrical Engineering and Computer Science, Seoul National University, Seoul, 3Department of Mathematics and Information, Gachon University, Gyeonggi-Do, 4Korea Electronics Technology Institute, Medical IT Technology, Gyeonggi-Do, Republic of Korea

Abstract: A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1–20 mM and a sensitivity of 7.66 µA mM-1 cm-2. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H2O2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.

Keywords: clinical diagnostics, glucose biosensor, metalloid-polymer nanoparticles, glucose oxidase, cyclic voltammetry

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