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Synthesis and evaluation of ortho-[18F]fluorocelecoxib for COX-2 cholangiocarcinoma imaging

Authors Chang CW , Yeh CN, Chung YH, Chen YR, Tien SW, Chen TW, Farn SS , Huang YC, Yu CS

Received 6 January 2018

Accepted for publication 14 March 2018

Published 24 May 2018 Volume 2018:12 Pages 1467—1478

DOI https://doi.org/10.2147/DDDT.S161718

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Anastasios Lymperopoulos



Chi-Wei Chang,1,* Chun-Nan Yeh,2,* Yi-Hsiu Chung,3,* Yong-Ren Chen,4 Shi-Wei Tien,4 Tsung-Wen Chen,2 Shiou-Shiow Farn,4,5 Ying-Cheng Huang,6 Chung-Shan Yu4,7

1Department of Nuclear Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; 2Department of Surgery, Liver Research Center, Chang-Gung Memorial Hospital at Linkou, Chang Gung University, Taoyuan, Taiwan; 3Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, Taoyuan, Taiwan; 4Department of Biomedical Engineering and Environmental Sciences, National Tsinghua University, Hsinchu, Taiwan; 5Isotope Application Division, Institute of Nuclear Energy Research, Taoyuan, Taiwan; 6Department of Neurosurgery, Chang-Gung Memorial Hospital at Linkou, Chang Gung University, Taoyuan, Taiwan; 7Institute of Nuclear Engineering and Science, National Tsinghua University, Hsinchu, Taiwan

*These authors contributed equally to this work

Background: An 18F-tagged NSAID analog was prepared for use as a probe for COX-2 expression, which is associated with tumor development.
Methods: The in vivo uptake of celecoxib was monitored with ortho-[18F]fluorocelecoxib using positron emission tomography (PET). The binding affinity of ortho-[18F]fluorocelecoxib to COX-1 and COX-2 enzymes were assessed using the competitor celecoxib.
Results: The IC50 values were 0.039 μM and 0.024 μM, respectively. A selectivity index of 1.63 was obtained (COX-2 vs COX-1). COX-2 overexpressed cholangiocarcinoma (CCA) murine cells took up more ortho-[18F]fluorocelecoxib than that by usual CCA cells from 10 to 60 minutes post incubation. Competitive inhibition (blocking) of the tracer uptake of ortho-[18F]fluorocelecoxib in the presence of celecoxib by the COX-2 overexpressed CCA cells and the usual CCA cells gave the IC50 values of 0.5 μM and 46.5 μM, respectively. Based on the in vitro accumulation data and in vivo metabolism half-life (30 min), PET scanning was performed 30–60 min after the administration of ortho-[18F]fluorocelecoxib through the tail vein. Study of ortho-[18F]F-celecoxib in the CCA rats showed a tumor to normal ratio (T/N) of 1.38±0.23 and uptake dose of 1.14±0.25 (%ID/g).
Conclusion: The inferior in vivo blocking results of 1.48±0.20 (T/N) and 1.18±0.22 (%ID/g) suggests that the nonspecificity is associated with the complex role of peroxidase or the binding to carbonic anhydrase.

Keywords: celecoxib, fluorination, imaging, NSAIDs, blocking, PET

Introduction

Cholangiocarcinoma (CCA) is a type of liver cancer that occurs in the epithelial lining of the biliary tract. Globally, it is the second most rapidly increasing malignant liver tumor.13 Although surgical resection is an effective treatment for CCA,46 the patient survival ratio remains very poor. The unmet medical needs for CCA include diagnosis at advanced stage, dismal prognosis leading to death of the patients within 1 year,7 and resistance to traditional chemotherapy and radiotherapy.

Recent studies have indicated that local inflammation around the biliary tree was highly associated with the epithelial transformation of the biliary tract from dysplasia to malignancy.8,9 The enzyme cyclooxygenase (COX) is crucial in such inflammatory cascades because it can catalyze the conversion of arachidonic acid to prostanoids.10 The inducible COX-2 enzyme is expressed in some human CCA cell lines upon inflammation.1113 In contrast, the homeostatic COX-1 enzyme, a housekeeping enzyme, regulates gastric acid in gastric mucosa. Therefore, COX-2 inhibitors or COX-2-specific binding compounds are considered reasonable targets for cancer therapy. The nonsteroidal anti-inflammatory drug (NSAID) such as aspirin has been reported to be inversely associated with the development of CCA. However, the biologic mechanism underlying prevention of CCA is plausible, especially by the COX-2 inhibitor.14 Although chronic use of high doses of selective COX-2 inhibitors such as coxibs, rofecoxib, and valdecoxib was associated with increased cardiovascular risk, celecoxib is still in use owing to an improved safety profile (Figure 1). Nevertheless, as COX-2 overexpression is associated with poor prognosis, celecoxib may still be of value for short-term treatment of COX-2-expressing tumors as a single drug or in combination with classic chemotherapeutic drugs or radiotherapy.15

Figure 1 Structures of the target compound ortho-F-celecoxib 1, the reported celecoxib, fluorolabeled celecoxib 2, 3, and other tagged NSAID analogs.
Abbreviation: NSAID, nonsteroidal anti-inflammatory drug.

The diagnosis tool positron emission tomography (PET) imaging provides metabolic information on drugs that have been tagged with second-period isotopic atoms, for example, 11C, 13N, 15O, and 18F. The introduction of an 18F atom moderately alters parent structure owing to its similar Van der Waals radius as hydrogen.16 Thus, the physiologic function of the parent compound can be retained. 18F is a positron emitter (t1/2=109.7 min; β+, 99%) with a coherent calibrating feature that can be coupled with PET to perform quantitative analysis, which is a unique characteristic among the current clinical imaging systems. PET imaging provides superior temporal and spatial resolution compared with single photon emission tomography and allows deduction of the concentration profile of the desired compound. 18F with an appropriate half-life has been labeled in celecoxib at different positions (Figure 1). However, there were a number of difficulties rendering the biologic results such as rapid in vivo defluorination 2–4,1719 unsuccessful in vivo blocking results 5,20,21 and lower specific binding affinity 6.22 11C-Labeled celecoxib analogs 7, 8 were more metabolically stable in vivo owing to the presence of ortho-fluoro group to resist its metabolism to form the carboxylic group.23,24 However, instead of a diaza five-member ring, the structurally altered imidazole ring may bias the molecular recognition. Other structural variation includes substitution of an ortho-chloro substituent for a meta-trifluoromethyl group. In addition, the short half-life of 11C may not be suitable for tracing longer metabolism. With respect to the pharmacokinetics that can timely evaluate a potential candidate molecule discovered from library screening, prompt tagging of 18F on this parent molecule would be meaningful. Hence, we are interested in preparing a fluoro-labeled celecoxib through a facial fluorination without changing structure. Compared with the nucleophilic fluorination that requires a tedious procedure for preparing the precursor, electrophilic radiofluorination on an arom ring of parent molecule is more straightforward. The in vivo PET imaging profile generated by tagged COX-2 inhibitors such as [18F]F-celecoxib could describe the affinity of celecoxib toward cancer; this information would be useful for quick assessment of its clinical potency.

Results and discussion

Preparation of ortho-[18F]F-celecoxib, [18F]F-1

Electrophilic fluorination using CF3COO[18F]F is a practical and rapid method for the introduction of 18F atom into celecoxib (Figure 2).25

Figure 2 (A) Preparation of ortho-F-1. (B) The less reactive CF3COOF was formed as the major fluorinating reagent.

The radiofluorination of celecoxib using a fluorine gas mixture of [18F]F2 and F2 produced the mixture of ortho-[18F]F-1, which could be primarily purified using high-performance liquid chromatography (HPLC; Figure 3). The radiochemical yield of the obtained fluorinated product ortho-[18F]F-1 was 9%. After the radioactive decay of 18F, the cold counterpart ortho-19F-1 was analyzed spectroscopically. A parallel cold fluorination was conducted with CH3COOF to generate ortho-F-1 for further spectroscopic comparison. The structures and purities were identified using nuclear magnetic resonance (NMR) and mass spectrometry. All the tagged samples were purified using preparative HPLC for the bioassays. However, the fluorination was not effective for the arom trans-resveratrol, a selective COX-1 inhibitor. It should be more reactive than celecoxib because of the electron-donating effect by the hydroxyl group. However, the relatively less reactive intermediate CF3COOF rather than the more reactive CH3COOF may address this result (Figure 2B).

Figure 3 The product mixtures obtained from the radiofluorination of ortho-[18F]F-1 were profiled using high-performance liquid chromatography as shown in the UV- (A) and radiochromatograms (B).
Notes: The fraction collected from tR of 19.97 min of the radiochromatogram was identified as ortho-[18F]F-1. Elution conditions: isocratic mode, RP-18 column, 70% EtOH (aq.), 3 mL/min flow rate, λ=254 nm. The isolated fraction was further confirmed using an analytic high-performance liquid chromatography system as shown in (C) UV- and (D) radiochromatogram. For clarification, an authentic celecoxib was added to the isolated fraction. The peak at tR of 7.80 min corresponds to a radiochemical purity of 99.92%. Elution conditions: isocratic mode, RP-18 column, 70% EtOH (aq.), 0.5 mL/min flow rate, λ=254 nm.

Tagged ligand-binding study

A common binding assay for COX is based on the inhibition of the conversion of a14C-labeled arachidonic acid to a 14C-prostanoid metabolite in the presence of competitors (Table 1). The binding assay for celecoxib was conducted by Uddin et al,19 who reported IC50 values of >4 and 0.03 μM for COX-1 and COX-2, respectively. An indirect enzyme-linked immunosorbent assay of PGE2 formation resulted in IC50 values of 3.7 μM (COX-1) and 0.06 μM (COX-2).26 Fenbufen analog is one of the NSAIDs and can be easily radiolabeled with isotopes for studying direct binding assay. Hence, the existing data from fenbufen analogs using direct binding and indirect binding assay can be compared with that of celecoxib. Their IC50 values are shown in Table 1.27 Direct assessment of the substrate–enzyme formation is relatively uncommon.28,29 Previous assay of the binding affinity (Kd) was performed with HPLC coupled with a gel filtration column. The tagged ligand–enzyme binding complex was differentiable from the free ligand.30,31 Because of the limited aqueous solubility of ortho-[18F]F-1, it was not suitable to use reversed-phase HPLC for binding study. Hence, a nonpolar liquid phase and a polar solid support, for example, silica cartridge, were employed to redistribute the polar tagged ligand–COX molecule and the nonpolar ligand (Figure 4).32 Through the nonlinear regression fit, the IC50 values of ortho-[18F]F-1 were determined to be 39.0 and 24.5 nM for COX-1 and COX-2, respectively. Our results showed a relatively inferior COX-2 selectivity (1.63) compared with the data from other groups. Interestingly, one of the direct binding assays using [3H]celecoxib gave a similar selectivity index of 1.79. The conformation of binding pocket of COX may vary when catalyzing. Direct binding assay measures an intimate contact with the active site. By contrast, the indirect binding assay measures the whole function involving the sequential catalysis from COX to peroxidase. Thus, the variable conformation may accommodate the substrate binding. COX-2 is better than COX-1 in tuning the active site for celecoxib analogs.

Table 1 Binding data obtained from this study and the literature
Abbreviation: COX, cyclooxygenase.

Figure 4 Plots of the formation of [18F]F–ligand–COX, ortho-[18F]F-1, in the presence of various concentrations of the competitor celecoxib (A and B).
Abbreviation: COX, cyclooxygenase.

In vitro tracer accumulation study

The binding affinity of the tracer ortho-[18F]F-1 was also assessed using the COX-2-overexpressed CCA cells33 and the usual CCA cells. The methods for preparing COX-2-overexpressed cell line have been reported before.34 The accumulation profiles of the two cells were different in the time course between 10 and 60 min (Figure 5). Hence, this temporal information was incorporated to the subsequent in vitro blocking study. The quick decline of the tracer uptake after 30 min may be due to the lipophilic metabolite resulting in a quick equilibrium across the cell membrane.

Figure 5 Tracer uptake of ortho-[18F]F-1 in the COX-2-overexpressed murine CCA tumor cells and the murine CCA tumor cells as a control.
Abbreviations: CCA, cholangiocarcinoma; COX, cyclooxygenase.

In vitro cellular binding study

Because the binding affinity of Coxibs to tumor cells is mainly challenged by their nonspecific binding to carbonic anhydrase, in vitro blocking study of ortho-[18F]F-1 was carried out to clarify its interaction specificity. The tracer accumulation by the COX-2-overexpressed cell was expected to vary upon the addition of the competitor of celecoxib with various concentrations. Thus, the two competitive inhibition profiles for the two cells were obtained (Figure 6). These various concentrations were generated from a serial dilution from mother liquor. However, the lipophilic celecoxib limits the choice of solvents. The most concentrated sample comprises 3% dimethyl sulfoxide (DMSO) and the rest of the dilutions contain <1% DMSO. Also, the tracer sink was diluted with H2O to lower DMSO concentration to <1/400. The toxic effect of DMSO is, therefore, negligible throughout both the tracer accumulation and the competitive inhibition studies. The two inhibition curves thus generated are interpolated to provide IC50 of 0.5 and 46.3 μM for COX-2 CCA and CCA, respectively. With a significant difference between the IC50 values of COX-2-overexpressed- and usual CCA cells, ortho-[18F]F-1 was intended to trace those COX-2-overexpressed CCA tumors in vivo.

Figure 6 Competitive inhibition of the tracer accumulation (ortho-[18F]F-1) in the presence of various concentrations of celecoxib (0.05 nM~250 μM).
Notes: In vitro system included COX-2-overexpressed CCA cells (A) and CCA cells (B). IC50=0.5 and 46.3 μM for COX-2-overexpressed CCA and usual CCA cells, respectively.
Abbreviations: CCA, cholangiocarcinoma; COX, cyclooxygenase.

In vivo tracer accumulation studies for CCA tumor-bearing rats

To evaluate the specificity of the tagged ligand toward tumor loci, PET was coupled with ortho-[18F]F-1 for CCA rats (n=4) and normal rats (n=2). The tumor loci was previously localized by PET with [18F]FDG (Figure 7A). Induction of CCA through administering thioacetamide (TAA) has been well characterized.35 Oral administration of TAA in drinking water to male Sprague Dawley (SD) rats results in a multistep model of biliary dysplasia and invasive CCA, which closely mimics human CCA. The development is fairly reproducible using this carcinogenesis model, with a 50% yield rate of invasive CCA by the 16th week; by the 22nd week, the yield of invasive CCA is 100%. The PET studies were initially performed using a dynamic mode from 0 to 60 min with a 10-min period to acquire the temporal profile of these tagged compounds at the region of interest (ROI). Thus, an optimal scanning period was determinable for subsequent static study. Along with the half-life (t1/2=30 min) and the sufficient number of animals for statistical comparison, systematic PET scans were established with the static mode from 30 to 60 min postinjection. Thus, the activity–time curves for ortho-[18F]F-1 were derived from a plot of the intensity of the circled ROIs obtained from the PET images vs time, as shown in Figure 7B and C. The steady accumulation of ortho-[18F]F-1 over the 1-h dynamic scanning period may indicate its selective uptake by COX-associated tumor cells followed by different release rates from the intracellular compartment to the extracellular compartment. The higher uptake ratio of [18F]FDG, which ranged between 2.2 and 2.4 (T/N), might be due to a longer circulation time that eliminated the background signals. In addition to inflammation-mediated accumulation, the formation of [18F]FDG-6-phosphate may assist its intracellular entrapment.36

Figure 7 (A) Selected PET images taken of two F18-tagged ligands in the same.
Notes: Data for [18F]FDG were obtained by scanning 90–120 min after intravenous injection into the tail vein. Data for ortho-[18F]F-1 were obtained by scanning 30–60 min postinjection. The red circles and white circles indicate the tumor loci and normal liver, respectively. (B) Activity–time curves of ortho-[18F]F-1 were constructed by counting the ROIs at the liver tumor loci and the adjacent normal region of the liver vs the scanning time over 1 h at 10-min intervals. (C) Dynamic PET studies of ortho-[18F]F-1 exemplified by coronal slices encompassing the liver of a CCA rat (left) and a normal rat (right) taken at 30–40 min in a 1-h scan. Red arrow bars indicate the ROIs circled for the tumor lesion (left) and the corresponding normal region (right), respectively. Number of rats used for the PET studies include n=4 for CCA rats and n=2 for normal rats.
Abbreviations: CCA, cholangiocarcinoma; PET, positron emission tomography; ROIs, regions of interest.

In vivo tracer blocking studies for CCA tumor-bearing rats

The in vivo binding specificity of a tagged compound is commonly assessed using a PET approach.37,38 We performed a comparative study using CCA rats treated with four doses ranging from 1 to 4 mg and using one normal rat treated with a dose of 4 mg. Mixtures of ortho-[18F]F-1 with different doses of celecoxib were coinjected to the rats per tail vein. Figure 8 represents a typical example of the obtained images. The results demonstrated a concentration dependency in the range of 1–3 mg of celecoxib (Table 2). However, marked variance was observed in the doses of 0 and 4 mg. The metabolism may be different between CCA rats and subsequently biasing the result (Figure 9). Because of the limited aqueous solubility, the relatively nontoxic solvent DMSO was used for dissolution. Therefore, to ensure animal survival, the maximal dose was limited to 4 mg, which may produce insignificant blocking.

Figure 8 A representative comparison between the static PET images of ortho-[18F]F-1 in a CCA rat (left) and in the same rat using cold celecoxib (2 mg) as a blocker (right).
Notes: The white arrows in a gross picture (middle) and in the two PET images indicate the tumor loci. The stronger signals in the PET images are comparable to the tumor lesions in the gross picture. The representative tumor grew in the upper-right lobe of liver. The blocking study using cold celecoxib did not result in signal reduction.
Abbreviations: CCA, cholangiocarcinoma; PET, positron emission tomography.

Table 2 Comparison between the blocking studies of CCA rats and normal rats in the presence of a competitor, celecoxib, at various doses
Abbreviations: CCA, cholangiocarcinoma; T/N ratio, tumor-to-normal ratio.

Figure 9 The dose–response curve for the CCA rats in the presence of the competitor celecoxib.
Abbreviations: CCA, cholangiocarcinoma; T/N ratio, tumor-to-normal ratio.

To summarize the results, ROIs circled in 13 normal liver regions derived from eight rats, including CCA rats and normal rats, and blocking experiments for both, were compared with the ROIs circled in nine tumor regions derived from five CCA rats and blocking experiments. The tracer uptake by tumor and normal tissues was 1.16±0.07 (%ID/g) and 0.87±0.04 (%ID/g), respectively (Figure 10).

Figure 10 Comparison between the tracer uptake of ortho-[18F]F-1 in the tumor lesion of CCA rats (n=5) and normal liver region of the rats including CCA rats (n=13), P=0.0021, one-tailed Student’s t-test. **The statistical variation is p<0.005.
Abbreviation: CCA, cholangiocarcinoma.

Conclusion

The radiofluoro analog of the selective COX-2 inhibitor celecoxib, ortho-[18F]F-1, was prepared using electrophilic fluorination. Through the direct binding assay of ortho-[18F]F-1 with COX-1 and COX-2 enzymes, submicromolar inhibition with slight COX-2 selectivity (1.63) was obtained. These results were in good agreement with other reported data. The indirect assay analyzes the COX–POX coupled function but not restricting to the sole COX binding. It was hypothesized that COX-2 accommodates celecoxib better than that by COX-1. COX-2-overexpressed CCA tumor cells took up more ortho-[18F]F-1 than that by CCA tumor cells from 10 to 60 min postincubation. Furthermore, COX-2-overexpressed CCA cells also showed higher specificity to ortho-[18F]F-1 than that by the usual CCA cells in an IC50 of 0.5 and 46.3 μM, respectively. PET studies of ortho-[18F]F-1 showed slightly higher uptake in CCA tumor compared with normal liver (1.16%ID/g vs 0.87%ID/g). The substrate accumulation in tumor cell is correlated with the initial COX recognition. However, the subsequent POX function may not play a significant role in the tumor metabolism. Hence, this may explain the moderate selectivity of ortho-[18F]F-1 (1.38±0.12, T/N) in vivo, whereas the in vitro binding data are even better. The lipophilicity of ortho-[18F]F-1 may increase its nonspecificity, and among which, carbonic anhydrase is the main enzyme responsible for this side effect.39,40 Induction and maintenance of a constant concentration of COX-2 may play a role in the therapeutics of celecoxib.

Experimental

Preparation of 4-(5-(3-fluoro-4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide (ortho-[18F]F-1)

Hot synthesis

A bottle of gas containing a mixture of F2/Ne (0.9%) was used to fill an Al target chamber with a volume of 750 cm3 to attain a pressure of 9.12 atm (134 psi), equivalent to 2.8 mmol of F2. Following bombardment with a beam line of deuterium of 8.5 MeV using an electric current of 40 μA for 2 h, the generated 18F-F2 gas was passed through a cartridge (5.6×35 mm) packed with a KOAc/HOAc (1:1.5) powder.25 The volatile mixture CH3COOF/[18F]CH3COOF (bp: 53°C) derived from the eluent (120 mCi) was bubbled through a solution of celecoxib (20 mg) (Matrix Scientific Co., Columbia, SC, USA) in trifluoroacetic acid (4 mL) for 25 min. The mixture was then concentrated under reduced pressure at 42°C for 2 min, 52°C for 2 min, and 65°C for 10 min to obtain an almost dry residue (0.1 mL). After mixing with aqueous 85% CH3CN (1.2 mL) for 2 min, the solution was passed through a 0.45 μm filter, and the filtrates were combined in a vial. The above procedure was repeated twice with aqueous 85% CH3CN (1.2 mL), and the two filtrates were combined in a new vial. An activity of 35.9 mCi in a volume of 2.2 mL was obtained 60 min postreaction. The complete mixture was subjected to HPLC purification with a flow rate of 3 mL/min using 70% EtOH (aq). The desired fraction at tR=19.97 min was collected. The isolated fraction was further identified with respect to its purity by using an analytic HPLC system. The retention time of 7.80 min corresponds to 99.92% radiochemical purity. After concentration under reduced pressure at 70°C, the obtained residue was mixed with DMSO (0.3 mL) and transferred to a plastic tube (3 mL). The mixing and transferring procedure was repeated three times, and further washes with saline (0.1 mL×4) and DMSO (0.1 mL) were carried out. The total wash volume (~1.5 mL) was combined in a tube as a stock for subsequent animal PET tests and radioligand-binding assays. A radiochemical yield of 9% (10.5 mCi) was obtained from the starting radioactive mixture (120 mCi) after 2 h and 10 min of decay correction (end of synthesis). An appropriate volume (0.1–0.3 mL) of the stock solution in a mixture of DMSO/saline (10:4) was drawn for each PET study to ensure a dosage of 0.6 mCi.

Cold synthesis of ortho-F-1

The same procedure was as described for ortho-[18F]F-1, except for the radioactivity. The subsequent nonradioactive experiment was conducted in a chemical hood. The white residue was further treated twice with toluene (3 mL) to remove the volatile solvents. A mixture of ortho-F-1 was obtained with a total yield of 10% (4 mg); according to the 1H-NMR integral ratios, the desired product ortho-F-1 constituted a yield of 8%. The sample was analyzed using 1H-, 13C-, and 19F-NMR as well as low-resolution and high-resolution electrospray ionization mass spectrometry (ESI-MS). The original spectra were shown in “Supplementary spectroscopic data” (Supplementary materials) 1H-NMR of ortho-F-celecoxib 1, “Supplementary spectroscopic data” (Supplementary materials) 13C-NMR (DEPT-135) of ortho-F-celecoxib 1, “Supplementary spectroscopic data” (Supplementary materials) 19F-NMR of ortho-F-celecoxib 1, “Supplementary spectroscopic data” (Supplementary materials) LR ESI-MS of ortho-F-celecoxib 1, and “Supplementary spectroscopic data” (Supplementary materials) HR ESI-MS of ortho-F-celecoxib 1. The following masses were calculated for C17H13F4N3O2S, [M+H]+ (m/z): 400.07 (100.0%), 401.08 (18.4%), 402.07 (4.5%); [M+Na]+ (m/z): 422.06 (100.0%), 423.06 (19.4%), 424.05 (4.5%); [2M+Na]+=821.12 (100%), 822.13 (37.2%); found ESI+Q-TOF, [M+H]+=400.07 (8.5%), 401.08 (1.7%); [M+Na]+=422.05 (56.6%), 423.06 (9.1%), 424.05 (1.7%); and [2M+Na]+=821.12 (5.5%), 822.12 (1.7%). For HRMS-ESI, calculated [M+H]+=400.0743; found [M+H]+=400.0741. 1H-NMR (500 MHz, CDCl3) δ 2.23 (d, 4JHF=1.5 Hz, 3H, −CH3, F-cel.), 2.31 (s, 3H, −CH3, Cel.), 4.89 (s, 2H, −NH2, F-cel.), 4.91 (s, 2H, −NH2, Cel.), 6.67 (s, 1H, arom., F-cel.), 6.69 (s, 1H, arom., Cel.), 6.79 (dd, J5″,6″=8.0, J5″,F=2.0 Hz, 1H, arom., 5″−H, F-cel.), 6.84 (dd, 3J2″,F=10.0, J2″,6″=1.5 Hz, 1H, arom., 2″−H, F-cel.), 7.04 (d, J=8.0 Hz, 2H, arom., Cel.), 7.11 (d, J=8.0 Hz, 2H, arom., Cel.), 7.12 (d, J6″,5″=8.0 Hz, 1H, arom., Cel., 6″−H, F-cel.), 7.39–7.42 (m, 2H, arom., Cel.+F-cel.), 7.82–7.87 (m, 2H, arom., Cel.+F-cel.). 13C-NMR (125 MHz, CDCl3) δ 14.46 (d, 3JCF=3.2 Hz, CH3, F-cel.), 106.37 (CH, arom., Cel.), 106.70 (CH, arom., F-cel.), 115.40 (d, JCF=24.2 Hz, CH, arom., F-cel.), 116.58 (q, 1JCF=293.8 Hz, CF3), 124.35 (d, 4JCF=3.1 Hz, CH, arom., F-cel.), 124.60 (CH, arom., F-cel.), 124.74 (s, CH, arom., Cel.), 126.84 (d, 2JCF=16.7 Hz, C, arom., F-cel.), 127.50 (CH, arom., Cel.), 127.62 (CH, arom., F-cel.), 127.76 (d, 3JCF=8.2 Hz, C, arom., F-cel.), 132.22 (d, 3JCF=5.5 Hz, CH, arom., F-cel.), 138.08 (C, arom., F-cel.), 139.81 (C, arom., Cel.), 141.27 (C, arom., Cel.), 141.59 (C, arom., F-cel.), 142.28 (C, arom., F-cel.), 142.59 (C, arom., Cel.), 144.05 (q, 2JCF=38.5 Hz, C, CCF3), 161.11 (d, 1JCF=245.8 Hz, C, arom., F-cel.). 19F-NMR (470 MHz, CDCl3)δ −62.50 (s, CF3), −115.06 (s, arom).

Radioligand-binding assay

An aliquot of COX-1 (80 μL, 1,600 units), COX-2 (200 μL, 1,560 units), or m-prostaglandin E synthase (125 μL, 275 units) was drawn from each of the respective commercial products and added to a plastic tube. Volumes of 1,920, 1,800, and 220 μL of Tris buffer (0.1 M, pH=8.0) were added to obtain a working concentration equivalent to 68 units/85 μL. A volume of 85 μL/well of the stock solution was added to a 96-well microtiter plate, followed by addition of the competitor celecoxib (10 μL) at concentrations of 0.002, 0.2, 2, 150, 400, 3,000, and 24,000 μM to yield a 10-fold dilution to produce final concentrations of 0.0002, 0.02, 0.2, 15, 40, 300, and 2,400 μM, respectively. The radiotracer solution of ortho-[18F]F-1 was prepared as a stock solution in EtOH (2×106 Bq/mL) obtained from the desired fractions after HPLC purification, and 5 μL (10,000 Bq) was added to each well. After standing for 10 min, the mixture in each well was transferred through pipette to a silica cartridge (Sep-Pak; Waters, Milford, MA, USA) followed by pumping through a blood pressure ball. The procedure was repeated by adding another portion of EtOH (0.5 mL). The two eluted portions were combined. The cartridge and filtrates were individually counted using an autogamma counter. The counts from both phases were used to deduce the binding percentage from the formula: counts (solid)/[counts (solid)+counts (effluents)]×100 (%). The displacement binding curve was obtained from a plot of the binding percentage vs the concentration of ortho-F-1 GraphPad Software, Inc., La Jolla, CA, USA). The IC50 value for the displacement binding of ortho-[18F]F-1 to COXs in the presence of celecoxib was obtained through manual adjustment. All experiments were performed in triplicate.

In vitro tracer uptake assay

Tracer uptake of ortho-[18F]F-1 was performed using COX-2-overexpressed CCA cells and usual CCA cells. Preparation of CCA cells has been reported earlier.33 The COX-2 expression pCDNA3 plasmid was obtained as a gift from Shyue, Song-Kun Lab, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. The transfection with COX-2 expression pCDNA3 plasmid using lipofectamine (Thermo Fisher Scientific, Waltham, MA, USA) was according to the manual protocol and selected by Geneticin (Thermo Fisher Scientific). The concentrate of ortho-[18F]F-1 generated from hot synthesis (3–5 mCi) was diluted to 1 mL using DMSO. An aliquot of 0.2 mCi was mixed with 20 mL of H2O in a polypropylene-based reservoir as shown in Figure S1 and Table S1. The two cells were seeded in a 96-well microtiter plate as a population of 10,000 per each well. An aliquot of 20 μL was transferred to each of the wells through a multichannel pipette, and the plate was placed on a water bath at 37°C. After various time points, the medium of each of the corresponding wells was removed, and 200 μL of distilled water was added for washing the residual medium. All the media were discarded. At 2 h postreaction, the cell pellets from all time points were detached by using 200 μL of 4N HCl (aq.), and the mixture each was transferred to a counting tube. After addition of H2O (2 mL), these tubes along with those tubes of in vitro blocking tests as described subsequently were counted by using a gamma counter made by 2470 Automatic Gamma Counter (PerkinElmer Inc., Waltham, MA, USA). The data were obtained in quadruple.

In vitro tracer blocking assay

A mother liquor of 50 mM of celecoxib in DMSO (2 mL) was first prepared in an Eppendorf tube. After dilution of an aliquot of 0.2 mL with 1.8 mL of aqueous DMSO (60%, v/v), the most concentrated competitor celecoxib (5 mM) was obtained. The subsequent serial dilution using H2O in 10–25-fold gave the next six samples, sequentially, with concentrations of 250 μM, 10 μM, 1 μM, 100 nM, 10 nM, and 1 nM. Each of the seven samples (100 μL) and ortho-[18F]F-1 (100 μL), derived from 0.2 mCi in 20 mL of H2O, were mixed in a polypropylene-based 96-well plate as shown in Figure S2 and Table S2. An aliquot of 20 μL of the mixture was transferred to each well of a cell-seeded 96-well microtiter plate, as described earlier. The concentration of celecoxib in the well of the plate was automatically diluted to 20-fold based on the presence of 200 μL of medium corresponding to 250 μM, 12.5 μM, 0.5 μM, 50 nM, 5 nM, 0.5 nM, and 0.05 nM. After standing at 37°C for 10 min, multiple mediums were removed using a multichannel pipette. The subsequent washing and removal of the attached cell pellets followed the same procedure as that described for tracer accumulation protocol. The data were obtained in quadruple.

Rat model

All in vivo experiments were conducted in compliance with the NHMRC Taiwan Code of Practice for the care and use of animals for scientific purposes41 and the Animal Use Protocols of Chang Gung Memorial Hospital. The study was approved by the Institutional Animal Care and Use Committee of Chang Gung Memorial Hospital (No 2013092702) and the Institutional Animal Care and Use Committee of Chang Gung University (No CGU12-055). Male SD rats aged 49 weeks were obtained from the Animal Research Center at Chang-Gung Memorial Hospital, Taiwan. The rats were housed under fixed environmental conditions and had free access to food and water throughout the experimental period.

Animals were fed regularly after recovery from anesthesia. They were carefully monitored with respect to the feeding quality, interaction, and symptoms of dystrophy. The animal care unit checked for abnormalities such as a feeding intake ratio <50% in 72 h, hind leg paraparesis, or a weight loss >20%. If the abovementioned situations occurred, the animal was sedated with isoflurane and xylazine hydrochloric acid and euthanized by CO2 and intravenous xylocaine (200 mg).

Induction of CCA through administering TAA has been well characterized.35 Briefly, oral administration of TAA in drinking water to male SD rats results in a multistep model of biliary dysplasia and invasive CCA, which closely mimics human disease. Similar to preneoplastic lesions described in human CCAs, the rat cholangiolar epithelium displays a phase of progressive “biliary dysplasia” preceding invasive cancer. In addition, both the precancerous and neoplastic biliary epithelia demonstrate foci of intestinal metaplasia (goblet cells), another well-known feature of the human counterpart. The strong, diffuse expression of biliary cytokeratin (CK19) confirms the bile ductular ontogeny of the neoplastic cells. The course of events is fairly reproducible using this carcinogenesis model, with a 50% yield rate of invasive CCA by the 16th week; by the 22nd week, the yield of invasive CCA is 100%. Notably, the occurrence of biliary dysplasia and invasive CCA precedes the development of hepatic fibrosis by 4 weeks, arguing against a “secondary” biliary proliferation in response to cirrhosis.

Small-animal PET imaging study of ortho-[18F]F-1

In vivo PET studies of ortho-[18F]F-1 were performed using TAA-induced CCA rats (n=5 at 37 weeks postadministration) and using normal rats as a control (n=3).32,35 These studies were performed at Taipei VGH. The rats were anesthetized through isoflurane inhalation (Forthane; Abbott Laboratories, Abbott Park, IL, USA) in oxygen (200 mL/min) during the imaging study.

A RODENT microPET R4 scanner (Concorde Microsystems Inc., Knoxville, TN, USA) was used for the small-animal PET scanning study. The crude data generated from the PET study were further processed using the Preclinical Multi-Modality Data Analysis software (ver 3.2; PMOD Technologies Ltd, Zurich, Switzerland).

For the dynamic PET study of ortho-[18F]F-1 (one CCA rat and one normal rat), the rats were first anesthetized with 3%–4% isoflurane, and the liver was positioned in the center of the field of view. After injection of 22.2±1.0 MBq of ortho-[18F]F-1 through the tail vein, a 1-h dynamic PET scan was performed to collect 10-min frames six times for each animal. The 10-min frames were either analyzed directly or binned together to obtain a 30–60-min scanning set. The six frames were used to plot activity–time curves with respect to the ROIs covering either the tumor sites or the adjacent normal regions vs the time course. The binned data sets from frame 4 to frame 6 could additionally serve as one static image frame (30–60 min). The static imaging mode was obtained from the imaging data over 30–60 min postinjection for both the tracer distribution study of CCA rats (n=4) and normal rats (n=2) and the blocking study of CCA rats (n=4) and normal rats (n=1). As described in the next section, the PET studies encompassed three normal rats and five CCA rats. The obtained images were reconstructed using two-dimensional ordered subset expectation maximization (OSEM 2D) and processed by PMOD 3.2 imaging analysis software. ROIs were drawn over the tumor with a threshold (maximum intensity minus minimum intensity)×50% and over the normal liver region. The average intensity in the ROIs was measured. Assuming a tissue density of 1 g/cm3, the unit of the ROIs (kBq/cm3) was converted to microcuries per gram and then divided by the administered activity to obtain an image ROI-derived percentage of the injected dose per gram of tissue (%ID/g).

PET study of ortho-[18F]F-1 in the presence of the blocking agent, celecoxib

Considering the limited aqueous solubility of ortho-[18F]F-1 and the toxicity of DMSO used to enhance the solubility, a maximal dosage of 4 mg of celecoxib was used in the blocking experiment. Doses of 1, 2, 3.4, and 4 mg of celecoxib were used. Mixing of the ortho-[18F]F-1 with various doses of celecoxib were then injected into CCA or normal rats. The static PET imaging studies were performed accordingly.

Acknowledgments

We acknowledge Mr Jun-Ming Chio, Mr Buo-Han Lin and Ms Yi-Ting Xie for their technical assistance. We also thank the Laboratory Animal Center, Chang Gung Memorial Hospital, Linkou for animals care and the Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, Linkou for PET analysis. We are grateful to the National Science Council of Taiwan, CGMH_NTHU Joint Research, and Chang-Gung Medical Research Project for providing financial support via the following grants: MOST-104-2113-M-007-019, MOST-97-2314-B-182A-020-MY3, MOST-97-2314-B-182A-020-MY3, MOST 103-2314-B-182A-081-MY2 and 105-2314-B-182A-041-MY2, CGTH96N2342E1, CMRPG3B0363, CMRPG3B0533, NMRPG5D6031~2, CMRPG3E1611~2, CRRPG3F0031~2, CMRPG390931, CMRPG3A0512, CMRPG3B0361, CMRPG6F0151, VGH105A-025 and NMRPG3F6021~2.

Author contributions

All authors contributed toward data analysis, drafting and revising the paper and agree to be accountable for all aspects of the work. All authors contributed toward data analysis, drafting and revising the paper and agree to be accountable for all aspects of the work.

Disclosure

The authors report no conflicts of interest in this work.


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