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Novel synthesizing method of pH-dependent doxorubicin-loaded anti-CD22-labelled drug delivery nanosystem

Authors Sun M, Wang J, Lu Q, Xia G, Zhang Y, Song L, Fang Y

Received 17 April 2015

Accepted for publication 29 May 2015

Published 7 September 2015 Volume 2015:9 Pages 5123—5133


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Wei Duan

Mengjiao Sun,1,* Jun Wang,1,* Qin Lu,1 Guohua Xia,2 Yu Zhang,3 Lina Song,3 Yongjun Fang1

1Department of Hematology/Oncology, Nanjing Children’s Hospital, Nanjing Medical University, 2Department of Hematology, Zhongda Hospital, Medical School, Southeast University, 3State Key Laboratory of Bioelectronics, Southeast University, Nanjing, People’s Republic of China

*These authors have contributed equally to this work

Abstract: The objective of this study was to investigate the anticancer efficacy of dimercaptosuccinic acid-modified iron oxide magnetic nanoparticles coloaded with anti-CD22 antibodies and doxorubicin (anti-CD22-MNPs-DOX) on non-Hodgkin’s lymphoma cells. The physical properties of anti-CD22-MNPs-DOX were studied and its antitumor effect on Raji cells in vitro was evaluated using the Cell Counting Kit-8 assay. Furthermore, cell apoptosis and intracellular accumulation of doxorubicin were determined by flow cytometry. The results revealed that anti-CD22-MNPs-DOX inhibited the proliferation of Raji cells, significantly increased the uptake of doxorubicin, and induced apoptosis. Therefore, it was concluded that a coloaded antibody and chemo­therapeutic drug with magnetic nanoparticles might be an efficient targeted treatment strategy for non-Hodgkin’s lymphoma.

Keywords: doxorubicin, anti-CD22 antibody, drug delivery system, target selection, non-Hodgkin lymphoma

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