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Design, Synthesis, And Evaluation Of Cyanopyridines As Anti-Colorectal Cancer Agents Via Inhibiting STAT3 Pathway

Authors Xu L, Shi L, Qiu S, Chen S, Lin M, Xiang Y, Zhao C , Zhu J, Shen L, Zuo Z

Received 30 May 2019

Accepted for publication 3 September 2019

Published 24 September 2019 Volume 2019:13 Pages 3369—3381

DOI https://doi.org/10.2147/DDDT.S217800

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Jianbo Sun



Lingyuan Xu,1–3,* Lingxi Shi,1–3,* Sensen Qiu,4,* Siyu Chen,1–3 Mengsha Lin,1–3 Youqun Xiang,1 Chengguang Zhao,3 Jiandong Zhu,2 Liqun Shen,4 Zhigui Zuo1,2

1Department of Colorectal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, People’s Republic of China; 2Department of Surgical Oncology, Affiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325600, People’s Republic of China; 3Cancer and Anticancer Drug Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People’s Republic of China; 4Department of Pharmaceutical Sciences, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Liqun Shen
Department of Pharmaceutical Sciences, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, People’s Republic of China
Tel +86 771 3267019
Email [email protected]
Zhigui Zuo
Department of Colorectal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, People’s Republic of China
Tel +86 577 86699057
Email [email protected]

Background: Colorectal cancer is one of the common malignant tumors. Cyanopyridine and aminocyanopyridine having a carbon-nitrogen bond have been shown to have significant anticancer effects. STAT3 is a promising therapeutic target in multiple cancers. However, there are currently no effective STAT3 inhibitors in clinical practice for the treatment of colorectal cancer.
Materials and methods: We screened 27 cyanopyridines for their anticancer activity by cell viability. The HCT-116, RKO, and DLD-1 cell lines were used to evaluate the anti-colorectal cancer effect of 3n. Scratch experiments and colony formation assays were used for the assessment of cell migration and proliferation capacity. Phosphorylated STAT3, STAT3, MCL-1, and Survivin levels were assessed by Western blot analysis.
Results: In this study, we synthesized 27 cyanopyridines and screened their anticancer activities in three human tumor cells, HCT-116, Hela229, and A375. We found that 2-amino-3-cyanopyridine 3n has better anticancer activity with IC50 values in the low micromolar range. Furthermore, 3n significantly inhibited the migration and colony formation of colorectal cancer cells. Mechanistically, 3n inhibited the expression of STAT3 phosphorylation in a dose- and time-dependent manner.
Conclusion: 3n is worth of further investigations toward the discovery of STAT3 inhibitor as a drug candidate for cancer therapy.

Keywords: design, cyanopyridine, colorectal cancer, STAT3, inhibitor

Introduction

Colorectal cancer is one of the common malignant tumors in our digestive system, and the morbidity and mortality rate are increasing year by year.13 At present, the main treatment for colorectal cancer is surgical treatment, combined with radiotherapy and chemotherapy, but surgery alone may not be cured.4 Drug treatment of colorectal cancer often has drug resistance, leading to a reduction in colorectal cancer survival rate for cancer treatment.5 Signal transduction and transcriptional activator (STAT3) is defined as an essential oncogene that regulates cancer cell proliferation, apoptosis, and metastasis.6,7 To a large extent, STAT3 is considered to be one of the key oncogenes and a key therapeutic target, so inhibition of aberrant activation of STAT3 in colorectal cancer may be a promising strategy.5,8,9 However, to date there have been no clinically useful STAT3 inhibitors for the treatment of cancer.1,10,11

There are many reports on the anti-tumor activity of 2-amino-3-cyanopyridines, but the results are not satisfactory.12 So far, none of these compounds have entered clinical research, and the structure–activity relationship of the system has not been given.1315 There are relatively few reports on 3-amino-3-cyanopyridine compounds compared to 2-amino-3-cyanopyridine compounds, and there are few studies on the anti-tumor activity of such compounds.16 Our group has previously demonstrated that the aminocyanopyridines exhibit significant antitumor activity against lung cancers in vitro and in vivo.17 So we continued our previous studies to further design, synthesize and evaluate the in vitro anticancer activity of a series of aminocyanopyridines.

Materials And Methods

Antibodies And Reagents

The antibodies against P-STAT3, STAT3, and GAPDH were purchased from Cell Signal Technology (Danvers, MA, USA). The horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). The caspase-3 colorimetric assay kit was purchased from Abcam Co. (Cambridge, MA, USA). A Bradford protein-assay kit, polyvinylidene fluoride membrane, and enhanced chemiluminescence kit were obtained from Bio-Rad Laboratories (Hercules, CA, USA). A protease phosphatase-inhibitor mixture was obtained from Applygen Technologies (Beijing, People’s Republic of China). Acrylamide (30%), coomassie brilliant blue, tetramethylethylenediamine, tris-glycine, sodium dodecyl sulfate, prestained protein marker, and nonfat dry milk were from Bio-Rad Laboratories.

Chemistry

Reactions were monitored by thin-layer chromatography on silica gel plates (HSGF 254), visualizing with ultraviolet. All the melting points are obtained uncorrected and were taken in a Microscope Melting Point X-6 (Beijing Tech, Beijing, People’s Republic of China). 1H NMR and 13C NMR spectroscopy were determined in Bruker ACANCE III HD-400 and 100 spectrometers (Bruker, Karlsruhe, Germany), respectively. Using DMSO-d6, acetone and CDCl3 solution with TMS as an internal standard. The following abbreviations mean spin multiplicities: singlet (S), doublet (D), triplet (T), and multiplet (M). IR spectra were recorded on a Fourier transform infrared (FTIR) spectrometer MAGNA-IR 550 (Thermo Electron Corporation, MMAS, USA) using KBr (υ cm−1). High resolution mass spectrometry (HRMS) were determined on LC-MS QE Focus (Thermo Fisher Scientific, MMAS, USA). Column chromatography was performed on silica gel (200–300 mesh) from Qingdao Ocean Chemicals (Qingdao, Shandong, People’s Republic of China) using distilled petroleum ether and ethyl acetate as mobile phase. Analytical HPLC analyses were performed on Agilent 1260 liquid chromatograph fitted with an Inertex-C18 column, with the purity of compounds >95%. All the materials were obtained from commercially available sources and used without further purification, unless otherwise specified. Yields were not optimized.

Cell Culture

Cell culture and human colorectal cell lines HCT-116, RKO, and DLD-1 were purchased from Shanghai cell bank. All of the above cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 media (Thermo Fisher Scientific, Waltham, MA, USA), containing 10% FBS (Gibco, NY, USA) and were maintained at 37°C in a humidified chamber with 5% CO2.

Cell Viability Assay In Vitro

Approximately 1×103 cells, suspended in 1640 medium, were plated onto each well of a 96-well plate and incubated in 5% CO2 at 37°C for 24 hrs. The test compounds at indicated final concentrations were added to the culture medium and the cell cultures were continued for 72 hrs. Fresh MTT was added to each well at a terminal concentration of 5 mg/mL and incubated with cells at 37°C for 4 hrs. The formazan crystals were dissolved in 150 mL DMSO each well, and the absorbency at 490 nm (for absorbance of MTT formazan) and 630 nm (for the reference wavelength) was measured with the ELISA reader. All the compounds were tested three times in each of the cell lines. The results expressed as IC50 (inhibitory concentration 50%) were the averages of three determinations and calculated by using the GraphPad Prism 6 software.

Colony Formation Assay

The untreated human colorectal cancer cells were plated in 6 well plates at a density of 1000 cells per well. After 7–10 days, the cells were fixed with 4% paraformaldehyde for 10–15 mins, colonies were stained with crystal violet for 15 mins at room temperature.

Cell Migration Assay

RKO and DLD-1 cells were seeded into 6 well plates at a density of 2×105 cells per well and cultured to 90% confluence. Then, the culture area was scratched with a sterile crystal pipette tip to make a linear gap in the confluent cell monolayer. The suspended cells were washed gently with PBS. Finally, cells were observed under an inverted microscope, cells were allowed to fill the gap and images of the culture area were captured at 0 hr, 24 hrs and 48 hrs after scratches. Three independent assays were performed.

Western Blot Analysis

The colorectal cells were lysed with RIPA lysis buffer with protease inhibitor (Boster, Wuhan, People’s Republic of China) and the protein concentration was determined after centrifugation at 12,000 rpm at 4°C for 10 mins. Supernatants collected, in order to determine total protein concentration, and protein concentrations assessed using a Bradford protein assay. The PVDF membrane was used to transfer proteins, and the blots were blocked for 90 mins at room temperature with fresh 5% nonfat milk in TBST, then the membrane with the specific primary antibody was incubated at 4°C overnight and at room temperature for 60 mins with the secondary antibody. Finally, the ImageJ software were used to quantify the bands.

Statistical Analysis

Statistics were performed using GraphPad Prism software (GraphPad, San Diego, CA, USA). The differences between sets of data were made using the Student's t-test. The results are expressed as mean±SE. P<0.05 was statistically significant.

Results

General Procedure For The Compounds 3a-3o (Scheme 1)

A mixture of the substituted acetophenone (1 mmol), together with the respective benzaldehyde (1 mmol), malononitrile (1 mmol), and ammonium acetate (8 mmol) in toluene (10 mL) was stirred at reflux for 8–12 hrs. After cooling to room temperature, the mixture was diluted ethyl acetate and THF. The organic phase was washed twice with brine, dried over Na2SO4, the filtrate and concentrated under reduced pressure. The residue was suspended in absolute ethanol. The precipitate was collected by filtration and purified by silica gel column chromatography to give the desired product.

Scheme 1 The designed route of the 2-amino-cyanopyridines and the chemical structure of the compound 3a-n.

2-Amino-6-(4-hydrophenyl)-4-(4-bromophenyl) nicotinonitrile (3a)

White floccule. mp: 304–307°C. 1H NMR (400 MHz, DMSO, δ ppm): 12.91 (s, 1H), 7.71–7.86 (m, 8H), 6.90 (s, 1H). 13C NMR (100MHz, DMSO-d6, δ ppm): 154.03, 149.28, 143.97, 136.83, 133.05, 132.15, 131.24, 123.73, 119.50, 116.15, 115.77, 105.10, 96.30, 95.25. IR (KBr) σ/cm−1: 3456.77, 3349.12, 2205.78, 1620.41, 1594.70, 1574.30, 1545.15, 1519.76, 1492.04, 1456.67, 1363.68, 1231.16, 1172.41, 805.80. HR-ESI-MS (pos. ion mode): m/z=366.0061[M+H]+ (anal. calcd. for C18H12BrN3O=365.0164).

2-Amino-4, 6-Bis-(4-bromophenyl) nicotinonitrile (3b)

White cottony. mp: 279–280°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 8.11 (m, 1H), 8.08 (m, 1H), 7.78 (d, J=2.0 Hz, 1H), 7.76 (m, 1H), 7.72 (m, 1H), 7.69 (m, 1H), 7.66 (m, 1H), 7.64 (d, J=2.0 Hz, 1H), 7.32 (s, 1H, cyanopyridine), 7.11 (s, 2H, amidogen). 13C NMR (101 MHz, DMSO-d6, δ ppm): 161.25, 157.95, 154.37, 137.09, 136.50, 132.17, 132.11, 131.03, 129.77, 124.40, 123.77, 109.47, 87.25. IR (KBr) σ/cm−1: 3494.14, 3389.24, 2205.50, 1607.41, 1580.65, 1543.00, 1489.62, 1454.13, 1392.56, 1431.69, 1364.77, 1073.69, 1009.54, 818.44. HR-ESI-MS (pos. ion mode): m/z=429.9371[M+H]+ (anal. calcd. for C18H11Br2N3=428.9299).

2-Amino-6-(5-fluoro-2-benzyloxy)-4-(4-bromophenyl) nicotinonitrile (3c)

Belge power. mp: 235–237°C. 1H NMR (400MHz, DMSO-d6, δ ppm): 7.71–7.72 (m, 1H), 7.69–7.70 (q, J =2.2 Hz, 1H), 7.64–7.65 (m, 1H), 7.42–7.44 (dd, J =7.7, 1.5 Hz, 2H), 7.26–7.38 (m, 8H), 7.05 (s, 2H), 5.15 (s, 2H, benzyloxy). 13C NMR (100MHz, DMSO-d6, δ ppm): 161.07, 156.49, 153.52, 153.01, 136.83, 136.49, 132.16, 130.56, 128.94, 128.69, 128.57, 123.58, 117.68, 117.18, 117.13, 115.47, 114.09, 86.74, 71.30. IR (KBr) σ/cm−1: 3470.51, 3319.99, 2919.08, 2206.72, 1648.36, 1572.28, 1544.44, 1501.00, 1452.65, 1426.09, 1253.56, 823.97, 727.52. HR-ESI-MS (pos. ion mode): m/z=474.0619[M+H]+ (anal. calcd. for C25H17BrFN3O =473.0539).

2-Amino-6-(2, 4-bisbenzyloxy)-4-(4-bromophenyl) nicotinonitrile (3d)

White cottony power. mp: 170–171°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.92 (d, J=8.6Hz, 1H), 7.64 (d, J=7.7 Hz, 2H), 7.49–7.36 (m, 10H), 7.25 (d, J=9.0Hz, 3H), 6.93 (s, 3H), 6.79 (d, J=8.7Hz, 1H, cyanopyridine), 5.18 (d, J=16.3Hz, 4H, benzyloxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.53, 161.05, 158.44, 157.73, 152.50, 137.19, 136.84, 136.77, 132.43, 132.10, 130.52, 128.97, 128.75, 128.58, 128.47, 128.37, 123.38, 120.00, 113.70, 107.26, 100.96, 85.26, 70.74, 70.00. IR (KBr) σ/cm−1: 3453.09, 3289.25, 3153.39, 2205.43, 1637.18, 1603.08, 1573.97, 1544.55, 1506.81, 1492.87, 1452.29, 1302.11, 1257.08, 1179.88, 1132.98, 1026.70, 822.96, 737.94, 694.70. HR-ESI-MS (pos. ion mode): m/z=564.1107 [M+H]+ (anal. calcd. for C32H24BrN3O2: 563.1031 (97.3%)).

2-Amino-6-(2, 4-bisfluoro)-4-(4-bromophenyl) nicotinonitrile (3e)

Belge power. mp: 235–238°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.76–7.47 (m, 6H), 7.30~7.26 (m, 1H), 6.99 (d, J=23.6 Hz, 2H), 6.82 (s, 1H, cyanopyridine).13C NMR (100 MHz, DMSO-d6, δ ppm): 154.03, 149.26, 143.96, 136.83, 133.00, 132.15, 131.22, 123.73, 119.51, 116.14, 115.76, 96.34, 95.28. IR (KBr) σ/cm−1: 3458.51, 3360.33, 3238.35, 2217.85, 1643.64, 1593.06, 1512.73, 1286.61, 1102.15, 813.55. HR-ESI-MS (pos. ion mode): m/z=386.0102[M+H]+(anal. calcd. for C18H10BrF2N3: 385.0026).

2-Amino-6-(4-hydroxyphenyl)-4-(3, 4-dimethoxyphenyl) nicotinonitrile (3f)

Yellow power. mp: 155–156°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 9.91 (s, 1H), 8.01 (d, J=8.5 Hz, 2H), 7.28–7.08 (m, 4H), 6.93–6.73 (m, 4H), 3.84 (d, J=5.5 Hz, 6H, methoxy).13C NMR (100 MHz, DMSO-d6, δ ppm): 161.35, 159.96, 158.90, 154.75, 150.39, 149.07, 129.88, 129.42, 128.92, 121.46, 115.83, 112.46, 112.12, 108.51, 85.63, 56.11. IR (KBr) σ/cm−1: 3465.71, 2923.19, 2201.05, 1640.74, 1610.51, 1575.25, 1546.32, 1513.00, 1260.11, 1207.93, 1207.93, 1170.39, 1130.99, 1021.82, 835.97. HR-ESI-MS (pos. ion mode): m/z=346.1202 [M-H]+ (anal. calcd. for C20H17N3O3: 347.1270).

2-Amino-6-(4-bromophenyl)-4-(3, 4-dimethoxyphenyl) nicotinonitrile (3g)

Brown power. mp: 125–127°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 8.10 (d, J=6.9 Hz, 2H), 7.71 (d, J=6.9 Hz, 2H), 7.30 (d, J=16.3 Hz, 3H), 7.14 (s, 1H, cyanopyridine), 7.02 (s, 2H), 3.79 (d, J=38.2 Hz, 6H, methoxy).13C NMR (100 MHz, DMSO-d6, δ ppm): 161.38, 157.59, 155.34, 150.54, 149.08, 137.30, 132.06, 129.75, 129.50, 124.20, 121.61, 117.78, 112.51, 112.11, 109.48, 87.31, 56.13, 56.11. IR (KBr) σ/cm−1: 3461.49, 2920.00, 2200.00, 1607.93, 1506.78, 1256.78, 1173.55, 1024.79, 837.36. HR-ESI-MS (pos. ion mode): m/z=410.0516 [M+H]+ (anal. calcd. for C20H16BrN3O2: 409.0426).

2-Amino-6-(2-benzyloxy-5-fluorophenyl)-4-(3, 4-dimethoxyphenyl) nicotinonitrile (3h)

White power. mp: 170–171°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.68 (d, J =9.1 Hz, 1H), 7.41–7.31 (m, 8H), 7.15 (s, 1H, cyanopyridine), 6.97 (dd, J =31.7, 13.3 Hz, 4H), 5.17 (s, 2H, amidogen), 3.84 (s, 3H, methoxy), 3.73 (s, 3H, methoxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.19, 156.27, 154.12, 150.42, 148.98, 136.97, 129.53, 128.88, 128.44, 128.31, 121.27, 117.64, 115.59, 114.19, 112.24, 86.90, 71.20, 59.13, 56.13, 56.03. IR (KBr) σ/cm−1: 3470.41, 3321.65, 3184.79, 2200.00, 1652.56, 1572.23, 1548.43, 1518.63, 1262.81, 1140.83, 1030.74, 807.60, 730.25, 688.60. HR-ESI-MS (pos. ion mode): m/z=456.1718[M+H]+(anal. calcd. for C27H22FN3O3: 455.1645).

2-Amino-6-(2, 4-bis (benzyloxy)phenyl)-4-(3,4-dimethoxyphenyl) nicotinonitrile (3i)

Belge power. 159–160°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.90 (d, J=8.7 Hz, 1H), 7.51–7.47 (m, 2H), 7.42 (t, J=7.4 Hz, 4H), 7.38–7.28 (m, 5H), 7.13 (d, J=2.1 Hz, 1H), 6.98 (d, J=8.4 Hz, 1H), 6.92 (d, J=2.3 Hz, 1H), 6.88–6.81 (m, 1H), 6.81 (m, 3H), 5.19 (d, J=2.9 Hz, 4H, benzyloxy), 3.84 (s, 3H, methoxy), 3.72 (s, 3H, methoxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.32, 161.12, 158.30, 157.49, 153.62, 150.26, 148.91, 137.23, 137.00, 132.40, 129.84, 128.96, 128.91, 128.33, 121.16, 120.49, 118.05, 113.84, 112.20, 107.24, 101.13, 85.56, 70.61, 69.99, 56.10, 56.00. IR (KBr) σ/cm−1: 3454.83, 3361.04, 2920.39, 2206.18, 1634.03, 1603.86, 1560.54, 1514.22, 1454.06, 1364.47, 1257.66, 1777.78, 1132.43, 1022.66, 822.00, 729.31, 693.45. HR-ESI-MS (pos. ion mode): m/z=544.2241[M+H]+(anal. calcd. for C34H29N3O4: 543.2158).

2-Amino-6-(2, 4-difluorophenyl)-4-(3, 4-dimethoxyphenyl) nicotinonitrile (3j)

White power. mp: 217–220°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.99 (d, J =7.5 Hz, 1H), 7.39 (t, J=10.5 Hz, 2H), 7.25 (dd, J=18.3, 9.5 Hz, 3H), 7.08 (d, J=42.4 Hz, 3H), 3.83 (s, 6H, methoxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 201.21, 185.06, 161.35, 150.60, 149.14, 139.96, 135.86, 132.99, 129.36, 121.47, 119.46, 112.36, 112.30, 112.27, 100.45, 90.45, 56.11. IR (KBr) σ/cm−1: 3359.21, 2924.70, 2205.84, 1625.63, 1572.65, 1544.24, 1518.05, 1267.17, 1133.89, 1099.48, 1026.41, 851.47, 791.36. HR-ESI-MS (pos. ion mode): m/z=368.1217[M+H]+ (anal. calcd. for C20H15F2N3O2: 367.1132).

2-Amino-4-(2-bromophenyl)-6-(4-bromophenyl) nicotinonitrile (3l)

Faint yellow power. mp: 162–164°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 8.07 (d, J=7.6Hz, 2H), 7.81 (d, J=7.2Hz, 1H), 7.70 (s, 2H), 7.50 (m, 3H), 7.23 (s, 1H), 7.14 (s, 2H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 160.51, 157.73, 138.62, 136.94, 133.26, 132.31, 132.16, 131.48, 130.96, 129.69, 128.47, 121.71, 116.42, 110.03. IR (KBr) σ/cm−1: 3483.25, 3362.95, 2214.86, 1632.91, 1572.02, 1546.87, 1446.52, 1270.71, 1007.51, 824.79, 754.01. HR-ESI-MS (pos. ion mode): m/z =429.9376 [M+H]+ (anal. calcd. for C18H11Br2N3: 428.9299).

2-Amino-6-(2-(benzyloxy)-5-fluorophenyl)-4-(2-bromophenyl) nicotinonitrile (3m)

Faint yellow power. mp: 146–148°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.78 (d, J =7.2 Hz, 1H), 7.72 (d, J=9.6 Hz, 1H), 7.47 (m, 2H), 7.30 (dd, J =16.6, 11.3 Hz, 9H), 7.09 (s, 2H), 5.15 (s, 2H, benzyloxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.52, 160.31, 158.31, 157.64, 153.74, 138.87, 137.18, 136.96, 133.18, 132.48, 131.18, 130.69, 128.96, 128.76, 128.37, 127.72, 121.69, 120.17, 114.32, 107.38, 101.20. IR (KBr) σ/cm−1: 3455.54, 3390.08, 3285.95, 2207.32, 1604.58, 1569.80, 1546.78, 1266.52, 1180.84, 1024.69, 731.73. HR-ESI-MS (pos. ion mode): m/z =476.0593[M+H]+ (anal. calcd. for C25H17BrFN3O: 475.0519 (97.3%)).

2-Amino-6-(2, 4-bis (benzyloxy) Phenyl)-4-(2-bromophenyl) nicotinonitrile (3n)

White power. mp: 171–173°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.93(d, J=8.7 Hz, 1H), 7.77 (s, 1H), 7.41 (m, 9H), 7.23 (d, J=17.5 Hz, 5H), 6.93 (s, 3H), 6.80 (d, J =7.3 Hz, 1H), 5.18 (d, J=11.1 Hz, 4H, benzyloxy), 4.35 (d, J=3.2 Hz, 2H).13C NMR (100 MHz, DMSO-d6, δ ppm): 160.35, 158.05, 156.39, 155.70, 154.23, 153.40, 138.57, 136.89, 133.23, 131.34, 130.69, 128.76, 128.42, 128.23, 127.82, 121.63, 117.94, 117.71, 117.28, 117.04, 116.41, 115.75, 115.67, 114.71, 88.91,71.09. IR (KBr) σ/cm−1: 3490.49, 3363.26, 2218.47, 1623.93, 1569.13, 1599.12, 1420.92, 1261.12, 1205.33, 1025.16, 780.48, 763.31, 753.71. HR-ESI-MS (pos. ion mode): m/z=564.1107[M+H]+(calcd. for C32H24BrN3O2: 563.1031 (97.3%)).

General Procedure For The Compounds 4a-4o (Scheme 2)

A mixture of the substituted acetophenone (1 mmol), together with the respective benzaldehyde (1mmol), ethylcyanoacetate (1mmol) and ammonium acetate (8mmol) in toluene (10mL) was stirred at reflux for 8–12 hrs. After cooling to room temperature, the mixture was diluted ethyl acetate and THF. The organic phase was washed twice with brine, dried over Na2SO4, the filtrate and concentrated under reduced pressure. The residue was suspended in absolute ethanol. The precipitate was collected by filtration and purified by silica gel column chromatography to give the desired product.

Scheme 2 The design route of 2-oxo-cyanopyridines and the chemical structure of the compound 4a-o.

4, 6-bis(4-bromophenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4a)

Yellow power. mp: 168–170°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 10.44 (s, 1H), 8.08 (d, J=8.0 Hz, 2H), 7.84 (d, J=7.4 Hz, 2H), 7.66 (s, 3H), 6.90 (d, J=8.0 Hz, 2H). 13C NMR (400 MHz, DMSO-d6, δ ppm): 187.47, 162.77, 141.80, 134.69, 132.30, 131.73, 131.10, 129.48, 124.09, 123.41, 115.87. IR (KBr) σ/cm−1: 3215.87, 2368.47, 1654.30, 1598.86, 1573.37, 1513.99, 1486.06, 1334.97, 1279.95, 1225.22, 1167.92, 812.66. HR-ESI-MS (pos. ion mode): m/z=367.0077[M+H]+(anal. calcd. for C18H11BrN2O2: 366.0004).

4-(4-bromophenyl)-6-(4-hydroxyphenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4b)

Faint yellow power. mp: 325–327°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 12.94 (s, 1H, lactam), 7.77 (m, 8H, aromatic), 6.91 (s, 1H, cyanopyridine). 13C NMR (100 MHz, DMSO-d6, δ ppm): 135.53, 132.34, 132.27, 130.92, 130.32, 125.48, 124.63, 116.72. IR (KBr) σ/cm−1: 3074.36, 2926.19, 2218.79, 1631.61, 1602.66, 1560.68, 1493.18, 1349.03, 1233.38, 1066.77, 1006.65, 816.63, 556.73, 494.32. HR-ESI-MS (pos. ion mode): m/z=430.9213[M+H]+ (calcd. for C18H10Br2N2O: 429.9139).

6-(2-(benzyloxy)-5-fluorophenyl)-4-(4-bromophenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4c)

Yellow needle power. mp: 118–120°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.71 (m, 1H), 7.54 (d, J =8.5 Hz, 3H), 7.33 (m, 8H), 7.21 (s, 1H), 6.99 (d, J=8.4 Hz, 2H), 6.73 (s, 2H), 5.14 (s, 2H). 13C NMR (400 MHz, DMSO-d6, δ ppm): 167.81, 166.22, 158.44, 154.72, 153.41, 150.78, 140.19, 136.03, 131.87, 131.51, 129.89, 128.90, 128.57, 121.60, 117.34, 116.91, 115.59, 106.06, 71.22. IR (KBr) σ/cm−1: 3415.51, 3272.92, 3173.49, 2985.27, 2247.60, 1680.28, 1615.98, 1572.50, 1491.94, 1282.43, 1250.94, 1192.61, 1108.16, 1011.47, 810.47, 735.28. HR-ESI-MS (pos. ion mode): m/z=475.0450[M+H]+ (calcd. for C25H16BrFN2O2: 474.0379).

6-(2, 4-bis (benzyloxy)phenyl)-4-(4-bromophenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4d)

Faint yellow power. mp: 165–168°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.91 (d, J=8.7 Hz, 1H), 7.41 (m, 13H), 7.13 (s, 1H), 6.97 (d, J=8.3 Hz, 2H), 6.70 (s, 2H), 5.17 (d, J=20.0 Hz, 4H, benzyloxy). 13C NMR (100 MHz, DMSO-d6, δ ppm): 167.98, 161.14, 158.59, 158.25, 150.78, 140.69, 137.27, 136.94, 132.24, 131.40, 129.87, 128.95, 128.92, 128.58, 128.44, 128.34, 121.35, 115.38, 107.15, 100.98, 70.64, 69.98. IR (KBr) σ/cm−1: 3422.83, 3266.96, 3168.02, 2918.61, 2360.65, 1680.43, 1603.46, 1571.60, 1488.09, 1258.68, 1186.16, 1100.30, 1021.82, 1011.92, 818.95, 735.55, 693.30. HR-ESI-MS (pos. ion mode): m/z=563.0972[M+H]+(calcd. for C32H23BrN2O3: 562.0892).

4-(4-bromophenyl)-6-(2, 4-difluorophenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4e)

Brown power. mp: 258–260°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 12.95 (s, 1H), 7.78 (m, 7H), 6.87 (s, 1H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.23, 160.10, 159.25, 153.75, 136.84, 132.14, 130.94, 129.47, 128.70, 123.54, 117.62, 115.88, 108.48, 85.45. IR (KBr) σ/cm−1: 3457.31, 3350.13, 2206.09, 1620.56, 1574.89, 1544.68, 1491.87, 1456.89, 1363.84, 1231.59, 1172.57, 805.77. HR-ESI-MS (pos. ion mode): m/z=386.9782[M+H]+ (calcd. for C18H9BrF2N2O: 385.9866).

6-(4-bromophenyl)-4-(3, 4-dimethoxyphenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4g)

Faint yellow power. mp: 309–312°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 12.75 (s, 1H), 7.80 (d, J =36.8 Hz, 4H), 7.36 (d, J=8.9 Hz, 2H), 7.14 (d, J=8.1 Hz, 1H), 6.89 (s, 1H), 3.85 (s, 6H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 151.27, 149.08, 132.31, 130.30, 128.45, 15.29, 125.26, 122.01, 112.41, 112.09, 56.20, 56.18. IR (KBr) σ/cm−1: 2933.95, 2838.96, 2220.79, 1632.59, 1598.04, 1515.67, 1494.22, 1421.33, 1261.98, 1217.85, 1148.23, 1021.81, 831.19. HR-ESI-MS (pos. ion mode): m/z =411.0172[M+H]+ (calcd. for C20H15BrN2O3: 410.0266).

6-(2-(benzyloxy)-5-fluorophenyl)-4-(3,4-dimethoxyphenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4h)

Faint yellow power. mp: 205–208°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 12.57 (s, 1H), 7.52–7.22 (m, J=30.3 Hz, 10H), 7.10 (d, J=7.7 Hz, 1H), 6.67 (s, 1H), 5.16 (s, 2H), 3.84 (s, 3H), 3.79 (s,3H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 154.24, 149.04, 136.92, 128.90, 128.86, 128.57, 128.42, 128.11, 128.08, 121.83, 115.35, 112.29, 112.28, 112.08, 82.75, 71.03, 71.02, 58.30, 56.16, 56.12. IR (KBr) σ/cm1: 3420.98, 2 927.97, 2838.63, 2221.89, 1654.48, 1603.88, 1518.79, 1262.70, 1146.03, 1032.76, 818.53, 730.35. HR-ESI-MS (pos. ion mode): m/z=455.1418[M-H]+ (calcd. for C27H21FN2O4: 456.1485).

6-(2,4-bis(benzyloxy)phenyl)-4-(3,4-dimethoxyphenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4i)

Faint yellow power. mp: 178–180°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.87 (d, J=7.8 Hz, 1H), 7.48–7.33 (m, 10H), 7.20 (s, 1H), 6.90 (s, 2H), 6.78–6.73 (m, 2H), 6.57 (d, J=6.0 Hz, 1H), 6.49 (s, 1H), 5.18 (s, 4H), 3.80 (s, 3H), 3.66 (s, 3H). 13C NMR (400 MHz, DMSO-d6, δ ppm): 168.65, 160.85, 158.13, 158.03, 155.71, 148.77, 137.11, 133.64, 128.95, 128.87, 128.41, 128.32, 128.24, 124.80, 124.74, 120.18, 112.11, 101.11, 70.51, 69.95. IR (KBr) σ/cm−1: 3427.79, 2961.41, 2922.03, 2360.35, 1679.82, 1603.49, 1562.38, 1508.49, 1457.52, 1260.44, 1104.97, 1025.11, 815.30. HR-ESI-MS (pos. ion mode): m/z=545.2073[M+H]+ (calcd. for C34H28N2O5: 544.1998).

6-(2, 4-difluorophenyl)-4-(3, 4-dimethoxyphenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4j)

Yellow power. mp: 253–255°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.85 (s, 1H), 7.42 (t, J=12.5 Hz, 1H), 7.25 (s, 3H), 7.11 (s, 1H), 6.65 (d, J=29.9 Hz, 1H), 5.31 (s, 1H), 3.83 (s, 6H), 3.82 (s, 6H). 13C NMR (100MHz, DMSO-d6, δ ppm): 150.73, 148.98, 133.00, 132.99, 132.93, 132.89, 121.58, 112.52, 112.30, 112.25, 112.09, 105.11. IR (KBr) σ/cm−1: 3444.10, 3055.13, 2919.16, 2847.54, 2360.58, 2215.93, 1656.98, 1617.30, 1520.05, 1271.72, 1023.85, 801.99. HR-ESI-MS (pos. ion mode): m/z=369.1045 [M-2H]+(calcd. for C20H15F2N3O2: 367.1132).

4-(2-bromophenyl)-6-(4-hydroxyphenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4k)

Brown power. mp: 132–135°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 9.88 (s, 1H), 7.98 (t, 1H), 7.95 (t, 1H), 7.67–7.55 (m, 1H), 7.46–7.41 (td, J=7.5, 1.2 Hz, 1H), 7.34–7.29 (m, 2H), 7.13 (s, 2H), 6.84 (s, 2H), 6.82 (d, J=1.9 Hz, 1H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 159.83, 158.23, 152.96, 143.30, 132.26, 131.17, 129.83, 129.45, 129.15, 128.79, 127.80, 121.70, 115.86, 110.41, 103.37. IR (KBr) σ/cm−1: 3470.22, 3413.54, 3369.47, 1686.21, 1599.93, 1570.73, 1516.70, 1360.58, 1282.87, 1230.64, 1169.10, 1102.44, 823.99, 755.09. HR-ESI-MS (pos. ion mode): m/z =367.0089[M+H]+ (calcd. for C18H11BrN2O2: 366.0004).

4-(2-bromophenyl)-6-(4-bromophenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4l)

Faint yellow cottony. mp: 298–300°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 13.00 (s, 1H), 7.79 (d, J=37.2 Hz, 4H), 7.51 (d, J=27.3 Hz, 3H), 6.81 (s, 1H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 137.74, 133.41, 132.41, 131.91, 130.52, 130.25, 128.58. IR (KBr) σ/cm−1: 3443.64, 2923.41, 2222.70, 1644.73, 1605.78, 1561.63, 1493.79, 1229.05, 1009.22, 823.69, 751.75. HR-ESI-MS (pos. ion mode): m/z=430.9051 [M+H]+ (calcd. for C18H10Br2N2O: 429.9139).

6-(2-(benzyloxy)-5-fluorophenyl)-4-(2-bromophenyl)-2-oxo-1,2-dihydropyridine-3-carbonitrile (4m)

Faint yellow power. mp: 167–169°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.96 (d, J=8.7 Hz, 1H), 7.64 (dd, J=7.9, 1.2 Hz, 1H), 7.50 (m, 2H), 7.42 (m, 2H), 7.33 (m, 3H), 7.08 (m, 3H), 6.92 (d, J=2.3 Hz, 1H), 6.80 (dd, J=8.7, 2.3 Hz, 1H), 5.19 (d, J=5.0 Hz, 2H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 161.26, 159.52, 158.26, 156.99, 151.91, 143.25, 137.23, 128.95, 128.77, 128.37, 128.37, 127.70, 121.64, 120.36, 115.88, 107.23, 103.34,101.12. 70.44. IR (KBr)σ/cm−1: 3424.16, 3263.82, 3164.77, 2924.13, 2357.69, 1677.35, 1603.39, 1569.37, 1261.04, 1186.93, 1109.95, 1025.89, 817.52, 733.30, 693.96. HR-ESI-MS (pos. ion mode): m/z=475.0450 [M+H]+(calcd. for C25H16BrFN2O2: 474.0379).

6-(2, 4-bis (benzyloxy) Phenyl)-4-(2-bromophenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4n)

White power. mp: 170–172°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 7.95 (d, J=8.3 Hz, 1H), 7.64 (d, J=6.3 Hz, 1H), 7.38 (dd, J=61.3, 24.8 Hz, 12H), 7.07 (s, 4H), 6.91 (s, 1H), 6.80 (d, J=7.9 Hz, 1H), 5.17 (d, J=19.3 Hz, 4H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 159.39, 152.13, 142.80, 136.91, 132.26, 129.58, 129.48, 128.90, 128.77, 128.20, 127.81, 121.56, 117.56, 117.34, 117.23, 116.98, 116.20, 115.65, 115.57, 104.85. IR (KBr) σ/cm−1: 3422.71, 3263.89, 3165.39, 2922.32, 2337.22, 1617.43, 1602.93, 1568.64, 1259.88, 1185.94, 1107.73, 1024.55, 817.10, 732.12, 693.23.HR-ESI-MS (pos. ion mode): m/z=563.0972[M+H]+ (calcd. for C32H23BrN2O3: 562.0892).

4-(2-bromophenyl)-6-(2, 4-difluorophenyl)-2-oxo-1, 2-dihydropyridine-3-carbonitrile (4o)

White power. mp: 300–302°C. 1H NMR (400 MHz, DMSO-d6, δ ppm): 13.09 (s, 1H), 7.80 (d, J=15.4 Hz, 2H), 7.55–7.47 (m, 4H), 7.28 (s, 1H), 6.61 (s, 1H). 13C NMR (100 MHz, DMSO-d6, δ ppm): 137.52, 133.47, 133.06, 132.96, 132.01, 130.47, 128.62, 120.87, 115.63, 112.95, 105.77, 105.50, 105.24. IR (KBr) σ/cm−1: 3443.52, 2778.95, 2221.46, 1650.66, 1614.21, 1506.77, 1269.52, 1226.79, 1110.34, 949.56, 853.61, 756.74. HR-ESI-MS (pos. ion mode): m/z=386.9782[M+H]+ (calcd. for C18H9BrF2N2O: 385.9866).

Screening Of Cyanopyridines Against Human Cancer Cells In Vitro

First, all the synthesized cyanopyridines were evaluated for their antiproliferative activity against a variety of cancer cells by the MTT assay in vitro (Tables 12). Among the cyanopyridines 3n exhibited potent inhibitory activity on all HCT-116, Hela, and A375 cells with the IC50 values of 10.50, 14.27 and 4.61 μM, respectively, which was the more potent compound for further study.

Table 1 IC50 of 3a-n for HCT-116, Hela229, and A375 (IC50 in μM)

Table 2 IC50 of 4a-o for HCT-116, Hela229, and A375 (IC50 in μM)

From the preliminary structure–activity relationships, we may conclude that introduction of benzyloxy group to the 1-position and 2-position of phenyl ring slightly enhance the cytotoxic activity, meanwhile bromine substituent to the 6-position on phenyl ring even had decreased activity compared to 3n. Furthermore, the compound 3n with 2-amino substituent was more active than 2-oxo congener 4n.

Active Compounds 3n Inhibited Migration And Colony Formation Of Colorectal Cancer Cells

Moreover, DLD-1 and RKO cells were treated with different concentrations of 3n to evaluate the effect of 3n on colorectal cancer cells migration (Figure 1A). The migration ability of cells treated with 3n was significantly reduced, which highlighted the critical role of 3n in inhibition of colorectal cancer cell migration. In addition, to observe the effect of 3n on the proliferation of colorectal cancer cells, we performed colony formation assays. Similar effects were observed in colony formation assays, 3n remarkably reduced the colony formation capacity in HCT116, RKO, and DLD-1 cancer cell lines (Figure 1B).

Figure 1 The compound 3n inhibits colony formation and migration of colorectal cancer cells. (A) In cell scratch test, a wound healing assay for migration by scraping cells with a yellow tip. The migration ability of RKO and DLD-1 cells was evaluated by a scratch test. Compared with the negative control, cells treated with different concentrations (10, 20, and 40 μM) of 3n showed that the cell migration was significantly slowed down with increasing concentration. Wound healing assay results from RKO and DLD-1 were calculated. (**P<0.01, ***P<0.001, ****P<0.0001). (B) For the colony formation assay, the number of colonies was counted 6–8 days after colorectal cancer cells were incubated and treated with various concentrations for 3n, and DMSO (1 μL) was added as a negative control and then stained with crystal violet.

The Compounds 3n Inhibited The Phosphorylation Of STAT3

Aminocyanopyridines can inhibit cell proliferation and induce cell death in human cancer cells by inhibiting the STAT3 pathway.17 We next validated the effects of 3n on STAT3 signaling in three colorectal cancer cells. As shown in Figure 2A, STAT3 phosphorylation was inhibited in a time-dependent manner with a 40 μM concentration of 3n, which significantly inhibited STAT3 phosphorylation at 24 hrs, but had no effect on total STAT3 levels in the three cell lines. Moreover, incubation of HCT116 with a concentration gradient of 3n (10, 20, and 40 μM), HCT116, RKO, and DLD-1 cells significantly downregulated phosphorylated STAT3 expression in a dose-dependent manner (Figure 2B). These results indicate that the anticancer activity of 3n is mediated through the inhibition of STAT3. As shown in Figure 2C, the results indicate that 3n, like the recognized role of the STAT3 inhibitor napabucasin, blocks IL6-induced activation of the STAT3 signaling pathway. We tested downstream protein levels of STAT3 and found that 3n also inhibits the expression of downstream target proteins, such as Survivin and MCL-1 (Figure 2D).

Figure 2 The compound 3n inhibited STAT3 phosphorylation in colorectal cancer cells. (A) The three colorectal cells were exposed to 40 μM 3n for different time periods (0, 2, 4, 8,12, and 24 hrs). Total protein was extracted, and the expression levels of P-STAT3, STAT3, and GAPDH proteins were detected by Western blot analysis. (B) Western blot analysis of STAT3 pathway associated proteins in colorectal cancer cells exposed to various concentrations (10, 20, and 40 μM) of 3n for 24 hrs. Representative pictures from three independent experiments are shown. (C) Human colon cancer cells were pretreated with 3n (10, 20, and 40 μM) and napabucasin (napa 1 μM) for 24 hrs and then stimulated with IL6 (25 ng/mL) for 30 mins. (D) The effect of 3n on Survivin and MCL-1 was analyzed by Western blot in RKO cells. (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001). Abbreviations: DMSO, dimethyl sulfoxide; STAT3, signal transducer and activator of transcription 3; P-STAT3, phosphorylated STAT3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MCL-1, myeloid cell leukemia-1.

Discussion

Colorectal cancer remains the leading cause of cancer deaths worldwide, despite extensive research and progress in screening and treatment.1,18,19 In addition, to date, there are no convincing agents that can significantly improve the survival rate of human colorectal cancer.5 STAT3 is considered to be an essential oncogene in the development of colorectal cancer and is thought to be carcinogenic in the development of colorectal cancer.20 In future studies, finding a suitable STAT3 inhibitor to inhibit abnormal activation of STAT3 in colorectal cancer cells remains a promising strategy to address this problem.21,22

In this study, we design of two new series of compounds with 2-amino-3-cyanopyridine chalcone and 3-cyano-2-pyridinone skeleton chalcone. Three kinds of human tumor cells HCT-116, Hela229, and A375 were screened for biological activity against proliferation. The results showed that 3n can effectively inhibit the proliferation and migration ability of colorectal cancer cells. Further studies confirmed that STAT3 phosphorylation was inhibited in a time- and dose-dependent manner after exposure to 3n. These results indicate that aminohydroxypyridine compound 3n can act as an inhibitor of STAT3, thereby inhibiting tumor growth and inducing apoptosis in colorectal cancer. 3n is a potent STAT3 inhibitor and may be a potential drug candidate for colorectal cancer therapy. Also, of course, 3n can also serve as lead compounds for optimization to speed the development of drugs selectively targeting the IL-6/STAT3 cancer signaling pathway. Additionally, the pharmacokinetics, pharmacodynamics, and toxicity of 3n will be further comprehensively evaluated. Combination therapy may enhance the curative effects and reduce the therapeutic concentration of chemotherapeutic drugs.23,24 We also plan to investigate the combination of 3n and other clinical cancer drugs to improve the antitumor potency of 3n and verify its effect on colorectal cancer.

Conclusion

In summary, a novel series of cyanopyridines and aminocyanopyridines were designed, synthesized, and biologically evaluated. The most potent compound 3n exhibited remarkable inhibitory activity on HCT116, Hela, and A375 cells. Furthermore, 3n significantly inhibits cell colony formation and migration of colorectal cancer cells. Mechanistically, 3n decreased protein expression level of P-STAT3Y. Together, 3n is worth of further investigations toward the discovery of STAT3 inhibitor as a drug candidate for cancer therapy.

Acknowledgments

This work was financially supported by the Zhejiang Provincial Natural Science Fund (LY18H160047), National Natural Science Funding of China (21762006), Wenzhou Science and Technology Project (Y20190179), and Major Science and Technology Projects of Guangxi Zhuang Autonomous Region (No. AA17204090).

Disclosure

The authors report no conflicts of interest in this work.

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