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Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity

Authors Patra JK, Das G, Shin HS

Received 17 April 2019

Accepted for publication 12 July 2019

Published 19 August 2019 Volume 2019:14 Pages 6679—6690

DOI https://doi.org/10.2147/IJN.S212614

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Jayanta Kumar Patra,1 Gitishree Das,1 Han-Seung Shin2

1Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of Korea

Correspondence: Han-Seung Shin
Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi 10326, Republic of Korea
Tel +82 31 961 5184
Email spartan@dongguk.edu

Background: The synthesis of silver nanoparticles (AgNPs) using food waste materials and their biomedical applications have garnered considerable attention recently.
Methods: Here, we investigated the synthesis of AgNPs using an aqueous extract of outer peel of Pisum sativum under different lighting conditions using standard procedures and explored their antidiabetic, cytotoxicity, antioxidant, and antibacterial potential.
Results: Characterization of AgNPs was done by Ultra Violet (UV-VIS) spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and at 464 nm for the samples exposed to direct sunlight, by scanning electron microscopy and energy-dispersive X-ray analysis that showed the surface nature and their elemental composition with a strong peak at 3 keV that corresponded to Ag (61.85 wt%), by Fourier-transform infrared spectroscopy that predicted the functional groups involved, and by X-ray powder diffraction that showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10–25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase (95.29% inhibition at 10 μg/mL and IC50 value of 2.10 μg/mL) and cytotoxicity (IC50 value 4.0 μg/mL as calculated from the slope equation) against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of 1,1-diphenyl-2-picrylhydrazyl at 100 μg/mL) and antibacterial activity against four human pathogenic bacteria (as indicated by 8.70–11.10 mm inhibition zones on agar plates).
Conclusion: In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have the potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments.

Keywords: antibacterial, antidiabetic, antioxidant, cytotoxicity, garden pea, silver nanoparticles, Pisum sativum

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