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Eye drop emulsion containing 0.1% cyclosporin (1 mg/mL) for the treatment of severe vernal keratoconjunctivitis: an evidence-based review and place in therapy

Authors Nebbioso M, Alisi L, Giovannetti F, Armentano M, Lambiase A

Received 17 April 2019

Accepted for publication 11 June 2019

Published 5 July 2019 Volume 2019:13 Pages 1147—1155


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Scott Fraser

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Marcella Nebbioso, Ludovico Alisi, Francesca Giovannetti, Marta Armentano, Alessandro Lambiase

Department of Sense Organs, Faculty of Medicine and Odontology, Umberto I Policlinic, Sapienza University of Rome, Rome 00185, Italy

Abstract: Vernal keratoconjunctivitis (VKC) is a rare, recurrent and multifactorial ocular disease, which typically flares up during spring and affects especially male children and adolescents. This condition does not usually respond to common treatments with antihistamines or mast cells stabilizers, whereas corticosteroids have effective results. Corticosteroids need to be carefully administered, to avoid adverse effects, mainly the secondary development of glaucoma, cataracts, or infections. Immunosuppressive agents, such as cyclosporin (CyA) or tacrolimus are, therefore, frequently employed in VKC patients. Only the 0.1% CyA (1 mg/mL) concentration has an approved and specific clinical indication for the treatment of VKC and this drug was given the denomination of orphan drug by the European Commission (EU/3/06/360) in 2006. So far, few studies have been conducted to evaluate the efficacy and the side effects of topical 0.1% CyA. Different topical CyA concentrations, ranging from 0.05% to 2%, and various types of formulation are available at the moment. In the future, 0.1% CyA will presumably take an important part in the management of VKC. The present review focuses on eye drops containing 0.1% CyA; however, more studies will be needed to define its long-term efficacy in the natural course of this severe ocular disease.

Keywords: atopy, conjunctivitis, cyclosporin, keratitis, ocular inflammation, vernal keratoconjunctivitis


Vernal keratoconjunctivitis (VKC) is a chronic and bilateral inflammation of the ocular surface mostly observed in the pediatric age group. The average age of onset of symptoms is 6–7 years and the male to female ratio is 4:1. The disease tends to resolve during puberty in most cases.1 The prevalence of VKC in Western Europe is estimated to be around 3.2/10,000 inhabitants. The disease is more common in dry and temperate areas, such as the Mediterranean area and central Africa; the prevalence in Italy is 27.8/10,000 inhabitants.2 VKC is usually seasonally exacerbated, in spring or early autumn, but it may also occur as a circannual disease. The most characteristic symptoms are itching, photophobia, watery eyes, foreign body sensation, and mucous secretions. The literature describes four types of VKC: the tarsal form, that affects mostly the tarsal conjunctiva with papillae sometimes with a cobblestone-like appearance; the limbal form, characterized by Horner-Trantas dots and multiple inflammatory nodules (Figure 1); the corneal form, with intense photophobia and alterations highlighted only with confocal microscopy (Figure 2), and the mixed type which has features of the other three types.3,4 Corneal involvement is not common, includes shield ulcers, punctate epithelial keratitis, corneal neovascularization, and can lead to the development of serious complications such as keratoconus or corneal perforation.4

Figure 1 Vernal keratoconjunctivitis (VKC): the tarsal form with giant papillae (left panel) and limbal form with Horner-Trantas dots (right panel, black and white arrows).

Figure 2 Vernal keratoconjunctivitis (VKC) corneal alterations as observed with confocal microscopy. (A) Larger and hyperreflective epithelial cells; (B) tortuous superficial nervous plexus; and (C–D) higher concentration of inflammatory cells and activated keratocytes.

VKC is included in the broad spectrum of allergic conjunctivitis. However, many different factors are involved in its pathogenesis, linked mainly to allergic and autoimmune diseases. Only 50% of VKC patients have a history of systemic atopic sensitization.5 The allergens involved differ geographically and not all of them can account for the seasonal exacerbations.6,7 Studies suggested that both IgE-dependent (type-I allergic) and IgE-independent (type-IV allergic) mechanisms may be involved in the immunopathogenesis of VKC.8 The role of type I allergic reactions has been highlighted by increased levels of IgE in serum and tears and increased number of mast cells in conjunctival tissue.9 An increased number of CD4+ Th2 cells and of their cytokines has been observed in tears and conjunctival biopsies, with the percentage of Th2 lymphocytes being associated with the severity of the disease.10,11

Extensive eosinophilia of the conjunctiva and high levels of IL-5 may contribute to tissue remodeling through the expression of high levels of TGF-β.12,13 A potential role of innate immune response has been highlighted by some authors. In fact, the increase of natural killer cells count and expression of toll-like receptors-4 in conjunctival biopsies have been recently demonstrated.14,15

The association of specific human leukocyte antigen (HLA) classes I and II has been investigated and has led to the finding of a characteristic overexpression of HLA-DRB1 and HLA-DQB1.16

Sex-hormone-related conditions such as gynecomastia have been reported in patients with VKC, suggesting the potential role of hormonal factors. This hypothesis is supported by positive staining for estrogen and progesterone receptors in conjunctiva and frequent resolution of symptoms after puberty.6,17 Moreover, male children with VKC have been shown to have decreased levels of dihydrotestosterone, a hormone able to alter the systemic immune response.18

The role of the immune system is also supported by the reduced levels of Vitamin D found in patients with active VKC and also by the fact that Vitamin D levels appear to be replenished after a few months treatment with 1% cyclosporin A (CyA).19 Several studies have suggested a strong relationship between Vitamin D levels and atopic and immuno-mediated diseases.20,21

Recent studies indicate that IL-17 may play an important role in the pathogenesis of VKC. VKC patients have higher levels of this cytokine compared to healthy subjects. IL-17 is involved in the development of autoimmune inflammatory diseases such as Sjӧgren’s syndrome, rheumatoid arthritis, systemic lupus erythematosus, and others.22,23

Lastly, antinuclear antibodies positivity, even though it is an unspecific parameter, was found in a relatively high proportion of VKC patients and could be linked to the severity of the disease.2426

The lack of a globally accepted grading system to standardize and classify the severity of VKC determines that the choice of medications varies greatly from physician to physician.

The use of grading scores to rate the severity of VKC and of an algorithm to select the most appropriate medications is crucial for management and to allow more extensive use of steroid-sparing agents such as CyA and tacrolimus.27

Well-defined management guidelines can lead to using less potent medications in the presence of mild disease and to switch to more potent medications for the more severe forms of disease.28

Sacchetti et al provide a five-tier classification for VKC. VKC is defined as quiescent in the absence of symptoms, mild in the presence of symptoms with no corneal involvement moderate (mild plus photophobia), severe (moderate adding superficial punctate keratopathy) and very severe (moderate adding diffuse superficial punctate keratopathy or corneal ulcer).29

Gokhale’s grading system provides a different five-tier classification that does not consider quiescent VKC and divides moderate VKC in intermittent, if the inflammation-free intervals (during which the patient is off medications) last >2–3 months and chronic if they last <1 month. In both classifications, corneal findings are more important than conjunctival findings.27

Several other scores such as the VKC-CLEK have been specifically developed to evaluate epithelial damage in VKC patients.30

Current treatment options for VKC include lubricants, antihistamines, and mast-cell stabilizers commonly used for mild disease.27 Severe to very severe VKC, especially with corneal involvement, is often treated with topical corticosteroids. Side effects of corticosteroid therapy such as glaucoma, cataracts and recurrent infections need to be strictly monitored.31 Lastly, CyA drops (0.05%, 0.1%, 0.5%, 1%, 2%) and tacrolimus (FK-506) 0.03% or 0.1% ointment are being developed for the treatment of VKC, but their role in each grade of VKC is still being evaluated.27,32

Cyclosporin A and currently available topical formulations

CyA is an 11-amino acid polypeptide, metabolite derived from the fungi Beauveria nevus and Tolypocladium inflatum, isolated in the early 1970s and initially used for its antifungal activity.33,34 It was not long before its more relevant immunosuppressive action was discovered. This cyclic hydrophobic neutral drug started to be employed as a treatment in organ transplantation35 and, later on, for inflammatory systemic diseases,36,37 due to the absence of significant bone marrow cytotoxicity.38 Its immunosuppressive role results from the inhibition of calcineurin 2,39,40 determining the block of transcription of nuclear factor of activated T-cells-dependent genes. This factor plays an important role in the synthesis of IL-2, fundamental for T-cell proliferation, IL-4 and CD40 ligand and essential for the activation of B-cell.41,42 CyA is also responsible for the inhibition of mast-cell degranulation and the transcription of IL-3, IL-5 and leukotriene.43

The introduction of CyA in ophthalmology dates back to the 1980s for the prevention of corneal graft rejection44,45 and subsequently for a large variety of inflammatory eye conditions, until it was approved by the United States Food and Drug Administration (FDA)46 in 2005 for the treatment of moderate to severe dry eye disease (DED), as confirmed by the latest DEWS report.77 The recent discovery of the anti-apoptotic effects of CyA has led to its use in ocular surface disorders such as VKC.47,48

From the pharmacokinetic point of view, it has been proved that local instillation of the drug does not require high concentrations of CyA in order to obtain an immunomodulating effect.49 Since there is low or no penetration in the humor aqueous, vitreous and ultimately in plasma, systemic side effects, such as nephrotoxicity and hypertension are practically absent.50,51 The hydrophobic structure of CyA has always represented a challenge, due to its poor aqueous solubility.52 In the form of eye drops, CyA was initially emulsionated using several vegetal oils, causing multiple side effects, ranging from uncomfortable symptoms, as conjunctival chemosis, itching or burning, to epithelial keratitis.53 New formulations, such as oil-in-water emulsion and micelle-based solution, are continuously being developed to avoid the aforementioned side effects.54 Different concentrations ranging from 0.05 to 2 mg/mL are available at the moment in different countries with different clinical indications (Table 1).55 Currently available products, main indications and country of distribution are summarized in Table 1. Finally, galenic formulations are available usually containing CyA in 1% or 2% concentrations in artificial tears.56

Table 1 Most common concentration of CyA eye drops formulations and countries of distribution

The lack of standardization related to both industrially produced and galenic formulations57 leads to several issues related to the development of reliable studies on CyA efficacy. To this day, the minimum effective concentration of CyA in VKC remains unknown.58

0.1% CyA in the treatment of VKC

VKC is a chronic, multifactorial, and remittent disease in which the activation of the immune system plays an active role. Symptoms can be controlled with steroid therapy, but treatment may be burdened by complications. In order to avoid as much as possible the use of cortisone, immunomodulators such as CyA may be used.5961

CyA at a concentration of 1 mg/mL (0.1%) has been recently developed and commercialized for the treatment of VKC (Tables 1 and 2).

Table 2 Summary of 0.1% (1 mg/mL) concentration CyA studies in VKC patients

Currently, only Papilock mini® and Verkazia® both produced by Santen (Osaka, Japan) are specifically indicated for the treatment of VKC (Table 1).

In 2009, Ebihara et al conducted a multicentric study in Japan in which they examined a total number of 594 patients, of whom 320 (median age 16 years) were affected by VKC and 274 by allergic keratoconjunctivitis. Signs and symptoms of disease were evaluated in order to build a 4-point grading scale.62 Topical 0.1% CyA solution was instilled three times daily in 90% of patients, twice daily in 5%, and four times daily in 5%, in one or both eyes. Only the most affected eye was considered for the study. Follow-up lasted 6 months. All scores for symptoms and signs significantly decreased from month 1 to month 6 of treatment in all three groups. Corneal involvement decreased from 21.6% to 8.6% and one-third of the patients were able to discontinue topical steroids. No significant adverse reactions to CyA were reported. The study had some limitations: the study was not randomized, there was no control group, no designated treatment, and there were no inclusion/exclusion criteria. Therefore, the improvement observed in some cases could be associated with the natural course of these diseases, which is characterized by remissions and exacerbations.62

Subsequently in 2010, Baiza-Duran et al published a double-masked, comparative, prospective, multicenter clinical study to evaluate the safety and efficacy of a 0.1% and 0.05% CyA solution in moderate to severe steroid-dependent children with VKC. Overall, 112 patients (mean age 10.2 years) were enrolled in the study after a complete washout period of the steroid therapy. Patients were randomized to one of 2 groups (0.05% or 0.1% CyA) and both eyes were treated simultaneously with one drop in each eye every 12 hrs. Signs and symptoms significantly improved in all patients during the 6 months of the study. Better results were seen in the first 2 months of treatment in the group of patients that received the 0.1% solution, especially when evaluating conjunctival chemosis and foreign body sensation. However, improvement in symptomatology showed no significant difference between the 0.1% group and the 0.05% group after 60 days of treatment. No adverse reactions to CyA were observed during the follow-up period in both concentrations.54 The authors suggest that the comparable efficacy of low CyA concentration may be attributable to the aqueous solution. This allows an increased bioavailability of CyA in the cornea.63 The results were consistent with the results obtained by Ebihara in VKC patients treated with a 0.1% CyA formulation with an aqueous vehicle.62,63

Two years after commercialization of the 0.1% formulation in Japan, Takamura et al followed up for 6 months 2,597 patients with VKC treated with this formulation and confirmed that it was safe and effective. Around 30% of patients who also concomitantly used topical steroids were able to discontinue the steroid treatment. Only mild adverse drug reactions were observed. The main limitation of this study is its observational design and the lack of a placebo control group.64

Lastly, a randomized multicentric Phase III trial, called VEKTIS, published in 2018 compared the efficacy of CyA drops in a cationic emulsion, instilled 4 times daily or 2 times daily, against the vehicle alone, instilled 4 times daily. One-hundred sixty-nine patients (mean age 9.2 years) with active severe VKC and severe keratitis participated. Severe VKC was graded with the Bonini scale (grade 3 or 4),3 severe keratitis with a score of 4 or 5 corneal fluorescein staining (CFS) on the modified Oxford scale.65

Patients were enrolled early during the allergy season in order to extend the follow-up period to 4 months to cover the whole VKC season and were randomized into 3 groups: CyA 4 times daily, CyA 2 times daily, and vehicle 4 times daily. At 4 months follow-up, both the high and low dose groups reported an improvement in symptoms and CFS. Steroid rescue medication use was more frequent in the vehicle only group. CyA therapy also determined an improvement in the subjective perception of disease, evaluated through a quality of life questionnaire.66 The overall frequency of adverse reactions was low and the therapy was well tolerated for the duration of the study. The main limitations of this study are: the enrollment of patients with severe VKC only, the short-term follow-up, and the use of the Oxford grading system, which has been specifically developed to evaluate corneal involvement in DED but not in VKC.67

To the best of our knowledge, no other studies on 0.1% CyA concentration nor any efficacy comparison with other CyA concentrations have been published.

Several studies have been conducted evaluating 2% CyA vs placebo. In all of them, CyA led to the improvement of VKC symptoms and was well tolerated.68,69 Moreover, 1% CyA has been proven effective for the treatment of VKC determining a decrease in the disease score severity after 2 weeks of therapy.70 Lastly, several trials on 0.05% CyA have found a consistent efficacy over placebo70,71 but not as a steroid-sparing therapy.72,73

In summary, 0.1% CyA has been proved to be effective in the treatment of VKC. Studies conducted have shown that patients treated with 0.1% CyA experience improvement of symptoms and signs, and in some cases are able to discontinue the steroid therapy. A consistent number of patients participated in the studies, allowing an adequate analysis of efficacy. However, only few studies have been published using this formulation and in three out of four studies reviewed, the enrolled patients belonged to a limited geographical area and ethnicity (Mexico and Japan). Only the VEKTIS study (2018) was a multicentric trial involving various European and extra-European countries. The follow-up in this study was 12 months, in which the authors analyzed disease severity scores, subjective symptoms and the need for steroid therapy. The follow-up period may be not long enough to allow an adequate evaluation over the course of the treatment because some patients show symptoms only during spring or summer while others present the symptomatology all the year.74,75


VKC is a recurrent disease, with exacerbations and remissions, therefore it is difficult to evaluate the persistence of efficacy over a long period of time. Also, the lack of diagnostic and prognostic biomarkers and of an internationally recognized grading score for VKC makes it difficult to define standardized clinical outcomes. The variability in clinical manifestations and severity of the disease even in the very short term can compromise evaluation of the therapy’s efficacy. Currently, the minimum CyA concentration to control symptoms of VKC is not known. In the future, it is auspicable that new studies be performed to compare 0.1% CyA concentration with higher doses, and using different formulations (such as oil-in-water or aqueous solution, that consistently affect the bioavailability of CyA), in order to establish the most effective and better-tolerated dosage and vehicle.76 Furthermore, a longer follow-up period is needed to evaluate the efficacy of CyA during the long natural course of the disease.

Ultimately, in the future, topical 0.1% CyA can be expected to play an increasingly important role in therapeutic strategies for severe VKC.


The authors have no proprietary interest in any materials or methods described in this article. The authors report no conflicts of interest in this work.


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