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Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Authors Chaves NL, Estrela-Lopis I, Böttner J, Lopes CAP, Guido BC, de Souza AR, Báo SN

Received 11 May 2017

Accepted for publication 28 June 2017

Published 2 August 2017 Volume 2017:12 Pages 5511—5523

DOI https://doi.org/10.2147/IJN.S141582

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Thomas Webster


Natalia L Chaves,1 Irina Estrela-Lopis,2 Julia Böttner,2 Cláudio AP Lopes,1 Bruna C Guido,1 Aparecido R de Sousa,3 Sônia N Báo1

1Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brasília, Brazil; 2Institute of Biophysics and Medical Physics, University of Leipzig, Leipzig, Germany; 3Institute of Chemistry, Federal University of Goiás, Goiânia, Brazil

Abstract: Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested.

Keywords: maghemite, nanomaterials, cells uptake, endocytosis

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