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Evaluation of the stability and antimicrobial activity of an ethanolic extract of Libidibia ferrea

Authors de Oliveira Marreiro R, Bandeira MF, Pereira de Souza T, Costa de Almeida M, Bendaham K, Venâncio GN, Costa Rodrigues I, Coelho CN, Milério PSLL, de Oliveira GP, Chacon de Oliveira Conde N

Received 11 September 2013

Accepted for publication 24 October 2013

Published 28 January 2014 Volume 2014:6 Pages 9—13

DOI https://doi.org/10.2147/CCIDE.S54319

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 5


Raquel de Oliveira Marreiro,1 Maria Fulgência Costa Lima Bandeira,1 Tatiane Pereira de Souza,2 Mailza Costa de Almeida,1 Katiana Bendaham,1 Gisely Naura Venâncio,1 Isis Costa Rodrigues,2 Cristiane Nagai Coelho,1 Patrícia Sâmea Lêdo Lima Milério,1 Glauber Palma de Oliveira,1 Nikeila Chacon de Oliveira Conde1

1School of Dentistry, Federal University of Amazonas, Manaus, Amazonas; 2School of Pharmacy, Federal University of Amazonas, Manaus, Amazonas, Brazil

Abstract: Biofilm is a dense, whitish, noncalcified aggregate of bacteria, with desquamated epithelial cells and food debris creating conditions for an imbalance of resident oral microflora and favoring the destruction of hard and soft tissues by development of caries and gingivitis. The aim of this study was to obtain and characterize an extract of Libidibia ferrea, ex Caesalpinia ferrea L. and to evaluate its feasibility for formulation as a mouthwash, according to current legislation. For this purpose, pH, sedimentation, density, and stability were evaluated, along with microbiological testing of the extract. The microbiological test was used to verify the presence of Staphylococcus aureus, Pseudomonas aeruginosa, fungi, yeasts, coliforms, and minimum inhibitory concentrations of Streptococcus mutans and Streptococcus oralis strains. Characterization, microbiological evaluation, and minimum inhibitory concentration results were tabulated and described using descriptive statistics. The L. ferrea extract showed stable characteristics, product quality, and antibacterial activity against the microorganisms tested irrespective of experimental time intervals. According to these results, it can be concluded that formulation of a mouthwash containing L. ferrea extract to control biofilm is feasible, but further studies are needed.

Keywords: biofilm, extract, quality control

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