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Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Authors Zheng S, Luo L, Bo R, Liu Z, Xing J, Niu Y, Hu Y, Liu J, Wang D

Received 24 June 2016

Accepted for publication 27 August 2016

Published 23 September 2016 Volume 2016:11 Pages 4891—4904

DOI https://doi.org/10.2147/IJN.S115742

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Lei Yang


Sisi Zheng, Li Luo, Ruonan Bo, Zhenguang Liu, Jie Xing, Yale Niu, Yuanliang Hu, Jiaguo Liu, Deyun Wang

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of China

Abstract: This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly(D,L-lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement.

Keywords: PHYP, optimal preparation condition, RSM, in vitro study

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