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Evaluation of in vivo antidiabetic, antidyslipidemic and in vitro antioxidant activities of hydromethanolic root extract of Datura stramonium L. (Solanaceae)

Authors Belayneh YM, Birhanu Z, Birru EM, Getenet G

Received 26 October 2018

Accepted for publication 5 February 2019

Published 23 April 2019 Volume 2019:11 Pages 29—38


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Bal Lokeshwar

Yaschilal Muche Belayneh,1 Zewdu Birhanu,2 Eshetie Melese Birru,2 Gedefaw Getenet1

1Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia; 2Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia

Background: The global morbidity and mortality rates of diabetes mellitus are persistently increasing. There is a demand for new antidiabetic drugs because the safety and efficacy of currently available medications are limited. The present study was therefore conducted to study the antidiabetic activities of the hydromethanolic root extract of Datura stramonium L. (Solanaceae) in mice.
Methods: Blood glucose lowering activity of three doses (100, 200, and 400 mg/kg) of the hydromethanolic root extract of D. stramonium was tested on normoglycemic, oral glucose-loaded, and streptozotocin (STZ)-induced diabetic mice models. The effect of the extract on body weight and diabetic dyslipidemia was also studied on STZ-induced diabetic mice. Additionally, antioxidant activity of the plant extract was determined using 2,2-diphenyl-1-picrylhydrazine free radical scavenging assay. Data were analyzed using one way ANOVA followed by Tukey’s post hoc multiple comparison test.
Results: The hydromethanolic root extract did not show significant hypoglycemic activity in normoglycemic mice. The plant extract at doses of 100, 200, and 400 mg/kg significantly (P<0.05) reduced blood glucose levels of oral glucose-loaded and diabetic mice. All the three doses of the root extract significantly improved diabetic dyslipidemia and the body weight of diabetic mice. Free radical scavenging activity of the root extract was found to be comparable to ascorbic acid with an IC50 of 13.47 µg/mL.
Conclusion: This study demonstrated that the hydromethanolic root extract of D. stramonium possesses significant antidiabetic, antidyslipidemic, and antioxidant activities.

Keywords: diabetes mellitus, Datura stramonium, antidiabetic, antidyslipidemic, antioxidant

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