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Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis

Authors Ruiz P, Causse M, Vaquero M, Gutierrez JB, Casal M

Received 26 July 2017

Accepted for publication 6 November 2017

Published 6 December 2017 Volume 2017:10 Pages 463—467

DOI https://doi.org/10.2147/IDR.S147272

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 3

Editor who approved publication: Professor Suresh Antony


Pilar Ruiz,1 Manuel Causse,2 Manuel Vaquero,1 Juan Bautista Gutierrez,1,2 Manuel Casal1,2

1Mycobacteria Reference Center, Department of Microbiology, Faculty of Medicine and Nursing, University of Córdoba, Córdoba, Spain; 2Microbiology Laboratory, Reina Sofía University Hospital, Córdoba, Spain


Abstract: A new automated real-time PCR assay for the detection of rifampicin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB) was evaluated. A total of 163 clinical samples (128 pulmonary and 35 extra-pulmonary) were processed using four PCR assay kits: Abbott RealTime MTB RIF/INH, Genotype MTBDRplus, Xpert/MTB RIF, and Anyplex MTB/MDR. The results of phenotypic drug-susceptibility testing using BACTECMGIT 960 were used as reference. The sensitivity and specificity of the new Abbott RealTime MTB RIF/INH assay in comparison with phenotypic testing was 96.3% (95%CI 87.32%–100%) for RIF and 100% (95%CI 99.3%–100%) for INH; the sensitivity was 78.8% (95%CI 66.8%–90.9%) and the specificity was 100% (95%CI 98.9%–100%). The Abbott RealTime MTB RIF/INH test could be a valid method for detecting the most common mutations in strains resistant to RIF and INH.

Keywords: Abbott RealTime MTB RIF/INH Resistance assay, M. tuberculosis, mutations

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