Back to Journals » International Journal of Nanomedicine » Volume 10 » Supplement 1 Challenges in biomaterials research

Eucalyptus oil nanoemulsion-impregnated chitosan film: antibacterial effects against a clinical pathogen, Staphylococcus aureus, in vitro

Authors Sugumar S, Mukherjee A, Chandrasekaran N

Received 8 January 2015

Accepted for publication 18 March 2015

Published 1 October 2015 Volume 2015:10(Supplement 1 Challenges in biomaterials research) Pages 67—75


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Thomas J. Webster

Saranya Sugumar, Amitava Mukherjee, Natarajan Chandrasekaran

Centre for Nanobiotechnology, VIT University, Vellore, India

Abstract: Eucalyptus oil (Eucalyptus globulus) nanoemulsion was formulated using low- and high-energy emulsification methods. Development of nanoemulsion was optimized for system parameters such as emulsifier type, emulsifier concentration, and emulsification methods to obtain a lower droplet size with greater stability. The minimized droplet diameter was achieved using the high-energy method of ultrasonication. Tween 80 was more effective in reducing droplet size and emulsion appearance when compared to Tween 20. Stable nanoemulsion was formulated with Tween 80 as a surfactant, and the particle size was found to be 9.4 nm (1:2 v/v). The eucalyptus oil nanoemulsion was impregnated into chitosan (1%) as a biopolymer in varying concentrations. Further, the film was characterized by moisture content, microscopic study, X-ray diffraction, and Fourier transform infrared spectroscopy. Also, the film with and without nanoemulsion was evaluated against Staphylococcus aureus. The nanoemulsion-impregnated chitosan film showed higher antibacterial activity than chitosan film. These results support the inclusion of nanoemulsion-impregnated chitosan film in wound management studies.

Keywords: essential oil, emulsion, biopolymer, impregnation, thin film, wound isolate

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]