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Eucalyptus oil nanoemulsion-impregnated chitosan film: antibacterial effects against a clinical pathogen, Staphylococcus aureus, in vitro

Authors Sugumar S, Mukherjee A, Chandrasekaran N

Received 8 January 2015

Accepted for publication 18 March 2015

Published 1 October 2015 Volume 2015:10(Supplement 1 Challenges in biomaterials research) Pages 67—75

DOI https://doi.org/10.2147/IJN.S79982

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster


Saranya Sugumar, Amitava Mukherjee, Natarajan Chandrasekaran

Centre for Nanobiotechnology, VIT University, Vellore, India

Abstract: Eucalyptus oil (Eucalyptus globulus) nanoemulsion was formulated using low- and high-energy emulsification methods. Development of nanoemulsion was optimized for system parameters such as emulsifier type, emulsifier concentration, and emulsification methods to obtain a lower droplet size with greater stability. The minimized droplet diameter was achieved using the high-energy method of ultrasonication. Tween 80 was more effective in reducing droplet size and emulsion appearance when compared to Tween 20. Stable nanoemulsion was formulated with Tween 80 as a surfactant, and the particle size was found to be 9.4 nm (1:2 v/v). The eucalyptus oil nanoemulsion was impregnated into chitosan (1%) as a biopolymer in varying concentrations. Further, the film was characterized by moisture content, microscopic study, X-ray diffraction, and Fourier transform infrared spectroscopy. Also, the film with and without nanoemulsion was evaluated against Staphylococcus aureus. The nanoemulsion-impregnated chitosan film showed higher antibacterial activity than chitosan film. These results support the inclusion of nanoemulsion-impregnated chitosan film in wound management studies.

Keywords: essential oil, emulsion, biopolymer, impregnation, thin film, wound isolate

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