Back to Journals » International Journal of Nanomedicine » Volume 12

Enhancing the oral bioavailability of biochanin A by encapsulation in mixed micelles containing Pluronic F127 and Plasdone S630

Authors Wu X, Ge W, Shao T, Wu W, Hou J, Cui L, Wang J, Zhang Z

Received 18 October 2016

Accepted for publication 11 January 2017

Published 22 February 2017 Volume 2017:12 Pages 1475—1483

DOI https://doi.org/10.2147/IJN.S125041

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Linlin Sun


Xiaoyan Wu,1 Weihong Ge,1 Tengfei Shao,1 Weijun Wu,1 Jian Hou,2 Li Cui,2 Jing Wang,2 Zhenghai Zhang2

1Department of Pharmacy, The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School, 2Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China

Abstract: Biochanin A (BCA), a natural dietary isoflavone, has been reported to show anticancer activities. However, its low biological availability and poor aqueous solubility limit its usefulness as a chemotherapeutic agent. We developed BCA-loaded micelles with Pluronic F127 and Plasdone S630 (BCA-FS). The optimized, spherical-shaped BCA-FS was obtained at a ratio of 1:1 (F127:S630). The particle size was 25.17±1.2 nm, and the zeta potential was −10.9±0.24 mV. BCA solubility in water increased to 5.0 mg/mL after encapsulation, and the drug-loading efficiency was 5.88%±0.76%. In vitro release experiments showed a delayed release of BCA from the mixed micelles. Furthermore, the BCA absorption permeability across a Caco-2 cell monolayer from the apical side to the basolateral side increased by 54% in BCA-FS. A pharmacokinetics evaluation showed a 2.16-fold increase in the relative oral bioavailability of BCA-FS compared with raw BCA, indicating that the mixed micelles may promote absorption in the gastrointestinal tract. A gastrointestinal safety assay was used to assess the reliability and safety of BCA-FS. On the basis of these findings, we conclude that this simple nanomicelle system could be leveraged to deliver BCA and other hydrophobic drugs.

Keywords: biochanin A, mixed micelles, oral bioavailability, Pluronic F127, Plasdone S630

Introduction

Isoflavone-rich foods and dietary supplements are garnering interest as agents for disease chemoprevention. Biochanin A (BCA; Figure 1A), a dietary isoflavone found in red clover, cabbage, and alfalfa, has been associated with various health benefits. As an anticancer compound, BCA was shown to be effective in reducing prostate,1 breast,2 and pancreatic3 cancer cell survival by acting on the cell proliferation and apoptosis pathways. In addition, owing to the structural similarities between BCA and estrogen, BCA can exert both estrogenic and anti-estrogenic effects4 and is marketed for the treatment of inflammation,5,6 osteoporosis,7,8 and other diseases.9,10 However, its clinical efficacy is limited by its low oral bioavailability, which is due to poor water solubility, high clearance, and large apparent volume of distribution.11 Hence, new delivery systems are required to improve the performance of BCA.

Figure 1 Chemical structure of drug and excipients.
Notes: (A) Structure of biochanin A; (B) structure of Pluronic F127; and (C) structure of Plasdone S630.

Nanostructured lipid carriers (NLCs) have been synthesized to improve the poor aqueous solubility and bioavailability of BCA.12 These NLCs are modified with polyethylene glycol (PEG) to enhance the surface hydrophilicity; the PEG chains are designed to reduce charge-based contact typical of proteins and small-molecule interactions.13 In general, NLCs are designed to be biodegradable and biocompatible. Nonetheless, they possess some limitations, such as manufacturing complexities and diseconomy in industrial production. Self-assembled micelle systems have also been shown to be a promising technique for improving delivery of poorly soluble drugs;14,15 the advantages of these systems include controlled drug release, enhanced drug solubility, and no immune response.16 Furthermore, the simple mechanism of formation is easy to apply during the manufacturing process.

In our design, a novel binary mixture of micelles was established using a self-assembly method by using Pluronic F127 (F127, Figure 1B) and Plasdone® S630 (S630, Figure 1C); our aim was to overcome the challenges of BCA’s poor oral bioavailability. The binary mixture shows favorable characteristics compared with a single-material system. Pluronic F127 consists of polyoxyethylene (PEO) units (70%) and polyoxypropylene (PPO) blocks (30%) and has a versatile triblock composition of PEO100–PPO65–PEO100. The hydrophobic PPO segments can form a hydrophobic core spontaneously, where hydrophobic drugs can be incorporated into the microenvironment. F127 is widely used in nanoparticle preparations because of its safety, long circulation period, and biocompatibility. However, its use is limited by its poor solubility and low stability, which make it difficult to use alone.1719 Plasdone S630 is a water-soluble organic polymer composed of polyvinylpyrrolidone (PVP) and vinyl acetate (VA), and has been widely applied in solid-dispersion formulations.2022 Because of the copolymerization, Plasdone S630 retains the merits of PVP, such as good solubility in water, good film formation, and better bonding performance, and incorporates extensive drug solubilization capacity and better surface activity derived from VA. Therefore, based on our previous studies,23,24 we speculated that the new combination of binary mixed micelles could enhance the aqueous solubility of BCA and improve its oral bioavailability.

In this study, BCA-loaded micelles with Pluronic F127 and Plasdone S630 (BCA-FS) were prepared by a simple self-assembly method. To evaluate the feasibility of the drug delivery system, we investigated the physicochemical properties of BCA-FS in vitro, including morphology, particle size, zeta potential, drug-loading capacity, and drug release. We also evaluated the absorption and efflux characteristics of BCA-FS in Caco-2 cell monolayers. Pharmacokinetics and gastrointestinal safety were assessed in vivo.

Materials and methods

Materials

BCA (98%) was purchased from Aladdin (Shanghai, People’s Republic of China). Plasdone S630 was obtained from Ashland (Covington, KY, USA). Pluronic F127 was provided by Sigma-Aldrich (St Louis, MO, USA). Caco-2 TC7 cells were a kind gift from Dr Ming Hu of INSERM U178 (Houston, TX, USA). Milli-Q water (Millipore, Bedford, MA, USA) was used throughout the study. Acetonitrile and methanol were obtained from Merck (Darmstadt, Germany). Tween 80 was obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, People’s Republic of China). All other chemicals were of analytical grade and were obtained from Tedia (Fairfield, OH, USA).

Animals

Sprague Dawley (SD) rats were obtained from the Experimental Animal Center of Shanghai (SLAS Lab, Shanghai, People’s Republic of China) and used for the pharmacokinetics study. Rats had access to food and water ad libitum, and were housed at a temperature of 25°C and relative humidity of 45% for 2 weeks. The use of these animals and cell lines were carried out in accordance with the guidelines of the Guide for the Care and Use of Laboratory Animals, which was evaluated and approved by the Institutional Ethics Committee of Jiangsu Provincial Academy of Chinese Medicine, who approved the overall study.

Preparation of BCA-FS micelles

BCA-loaded mixed micelles were prepared according to a self-assembly technique as previously described.23 In brief, BCA was dissolved in ethanol at a concentration of 5 mg/mL. F127 and S630 were combined in different ratios and dispersed in ethanol at 45°C. The F127/S630 ethanol solution was stirred uniformly until it reached equilibrium. The BCA ethanol solution was then added dropwise into the F127/S630 solution with magnetic stirring. After 1 h of gentle stirring at 25°C, the ethanol was removed under reduced pressure at 45°C. Deionized water was added to the flask, and a transparent solution formed after shaking. The mixed micelles were then acquired after ultracentrifugation at 14,000 rpm (18.8× g) for 10 min. Finally, the supernatant was retained and the stability of the mixed micelles was assessed.

Characteristics of BCA-FS micelles

Particle size and zeta potential analysis

Mean particle size, polydispersity index (PDI), and zeta potential of BCA-FS in the dispersion were determined in triplicate by dynamic light scattering using a Nano ZS system (Malvern Instruments, Malvern, UK) at 25°C. The mixed micelles were diluted fivefold with water for injection before measurement. Zeta potential measurement was carried out using a zeta potential analyzer.

Transmission electron microscopy

BCA-FS morphology was observed using transmission electron microscopy (TEM, JEM-200CX; Jeol, Tokyo, Japan). BCA-FS droplets were applied to a copper grid and then stained with 2% (w/v) phosphotungstic acid for 30 s. The grid was observed using TEM after infrared drying for 5 min.

Determination of drug loading

Drug loading was determined by high-performance liquid chromatography (HPLC), which consisted of a pump (G1311G; Agilent Technologies, Palo Alto, CA, USA), a UV detector (G4212B; Agilent Technologies) set at 260 nm, an autosampler (G1329B; Agilent Technologies), and a reversed-phase C18 column (4.6×250 mm, 5 μm, SunFire; Waters Co., Milford, MA, USA). The mobile phase, with a flow rate of 1.0 mL/min, consisted of a mixture of acetonitrile and 0.1% phosphoric acid solution (50:50, v/v), which was freshly prepared and filtered with a 0.45 μm membrane. BCA-loaded dried micelles were obtained after freeze-drying. Weighed amounts of dried micelles were then dissolved in methanol and fragmented by ultrasonication. The entrapment efficiency (EE) and drug-loading (DL) content of BCA were calculated using the following equations:


(1)


(2)

In vitro release experiment

The dialysis bag method was used to estimate the drug release from BCA-FS in vitro; this method retains micelles and allows the free BCA into the dissolution media with a molecular weight cut-off of 3,500 (Greenbird, Inc., Shanghai, People’s Republic of China). The bags were soaked in double-distilled water for 12 h before use. Next, 1 mL of BCA-FS was poured into the bag and the two ends fixed by clamps. The bags were placed into conical flasks containing the receiving phase, which consisted of 200 mL phosphate-buffered saline (PBS, pH 6.8) at 37°C and 0.5% (w/v) Tween 80. The conical flasks were placed into a thermostatic shaker (HZQ-C; Shuangjie, Changzhou, People’s Republic of China) at 37°C and shaken at a rate of 140 times per min. At 1, 2, 3, 6, 8, 20, 44, and 72 h after beginning the test, triplicate suspensions (0.5 mL) of the release medium were removed and filtered through a 0.45 μm membrane for analysis, while fresh dialysis medium was added to maintain sink conditions. The filtrate was analyzed using HPLC at the different time points.

Transport experiments in Caco-2 cell culture model

The Caco-2 TC7 cell line, similar to wild-type Caco-2 cells, was selected to investigate BCA absorption in this study owing to its stability. Culture conditions for growing Caco-2 cells were the same as in our previous studies.23 Briefly, Caco-2 cells were grown in high-glucose Dulbecco’s Modified Eagle’s Medium at 37°C in a 5% CO2 atmosphere and at a relative humidity of 95%, supplemented with 10% fetal bovine serum, 1% minimal essential medium non-essential amino acids, 2 mM L-glutamine, and 1% penicillin/streptomycin. Cells were seeded at a density of 1×106 cells/cm2 on untreated culture inserts of polycarbonate membranes with 0.4 μm pores. Culture inserts containing a Caco-2 monolayer were placed in 12-mm transwell plates. Approximately 21 days after seeding, the cells were fully differentiated and transepithelial electrical resistance (TEER) values became stable (>400 Ω/cm2). Transport studies were not carried out until the cell monolayer was formed.25

Caco-2 transport studies were initiated by removing the medium from both sides of the monolayer, followed by replacement with apical (AP) and basolateral (BL) buffers prewarmed to 37°C. The cell monolayers were incubated in a shock shaker for 30 min at 37°C (100 cycles/min) until equilibration. After aspirating the incubation medium, a solution of dissolved mixed micelles loaded with 20 μM BCA was added to the AP or BL side. At fixed time points (0, 1, 2, 3, and 4 h after incubation), 400-μL aliquots were taken from both the donor and receiver sides, and the same volume of donor solution (containing BCA) and transport medium (Hank’s balanced salt solution) was replaced after each sampling. One hundred microliters of internal standard solution (chloramphenicol [CM] 100 μM in methanol) were immediately added to samples to stabilize them until analysis. These samples were then centrifuged at 16,000 rpm for 5 min at 4°C, and the supernatant was analyzed using HPLC. TEER values were measured at the end of the transport experiment and compared to the TEER values measured at baseline. The BCA permeability coefficient was calculated from the linear plot of the quantity transported versus time, using the following equation:


(3)

where C0 is the initial concentration of BCA on the donor side, S is the surface area of the cell monolayer (4.2 cm2), and dQ/dt is the steady-state appearance rate of BCA on the receiver side.

Gastrointestinal mucosa toxicity test

SD rats (average weight 220 g) were randomly divided into two groups and treated with normal saline (control group) or BCA (20 mg/kg) three times per week for 1 month. Mice were killed and 1 cm segments of stomach and small intestine were excised from the gastrointestinal tract. These tissues were fixed in 10% formaldehyde-based fixative for 24 h, rehydrated, and then embedded into paraffin. Next, 5 μm sections of these tissues were stained with hematoxylin and eosin (H&E). The samples were imaged with a light microscope.

Pharmacokinetic studies

Experimental process and sample treatment

For the pharmacokinetic study,26,27 SD rats (weight 220±10 g) were divided randomly into two groups (n=6). All rats were fasted overnight to prevent coprophagia, but had free access to water. The first group received the BCA suspension orally, dispersed in 0.1% aqueous sodium carboxymethyl cellulose; the second group received 50 mg/kg of BCA-FS. All oral formulations were diluted with water immediately prior to administration. Blood samples were collected from the retro-orbital plexus in tubes containing heparin at 0, 5, 15, and 30 min, and at 1, 1.5, 2, 3, 4, 5, 6, 8, 12, and 24 h following treatment. The blood samples were centrifuged for 10 min at 10,000 rpm, and the supernatant plasma fraction was stored at −80°C until ultra-performance liquid chromatography (UPLC) analysis.

The supernatant pretreatment was conducted by mixing 130 μL of plasma sample with 20 μL of the internal standard CM (13.858 ng/mL in methanol). After vortexing, the liquid–liquid extraction was accomplished by adding 1 mL of ethyl acetate, followed by gentle agitation for 4 min. The mixture was centrifuged for 5 min at 8,000 rpm, and 800 μL of the organic layer was then transferred to a clean vial and evaporated until dry. Finally, the residue was dissolved in 520 μL of the initial mobile phase, transferred to autosampler vials, capped, and placed in the UPLC autosampler. Calibration curves were used to convert the BCA/CM chromatographic area to the concentration. The plasma BCA concentration versus time plot was obtained and analyzed for pharmacokinetic parameters.

Chromatographic and mass spectrometry settings

A Waters Acquity UPLC System (Waters Co.) was used for chromatographic analysis, consisting of a binary pump solvent management system, an online degasser, and an autosampler. Chromatographic separation was carried out at 30°C on a reversed-phase C18 column (100×2.1 mm, 1.7 μm). The mobile phase, pumped at 0.3 mL/min, was composed of A (acetonitrile) and B (water) using a gradient elution of 38% A at 0–3 min, 100% A at 3.2–4.2 min, and 38% A at 4.4–5.0 min. The injection volume was 5 μL.

Mass spectrometry was carried out on an electrospray ionization source in the negative mode. The parameters of the source were set as follows: source temperature, 150°C; desolvation temperature, 500°C; cone gas flow, 50 L/h; desolvation gas flow, 1,000 L/h; capillary voltage, 3.3 kV. The dwell time was automatically set by the software. Analytic detection was performed using the multiple reaction monitoring mode at m/z transitions 282.77→267.84 for BCA, and 320.77→151.81 for CM.

Statistical analysis

Data are expressed as the mean ± standard deviation of at least three experiments. In all cases, P<0.05 was considered statistically significant. A Student’s t-test was used to determine statistically significant differences between the experimental groups. Data analysis was performed using Statistical Package for the Social Sciences (SPSS) 18.0 (SPSS, Armonk, NY, USA).

Results and discussion

Physicochemical characterization

A range of single-factor experiments was performed to investigate the impact of different proportions of F127 and S630 on the preparation of micelles. The micelles showed beneficial properties at a ratio of 1:1 (400 mg F127 to 400 mg S630). The mean diameter of BCA-FS was 25.17±1.2 nm, and the PDI was 0.147±0.011. The zeta potential was −10.9±0.24 mV, which could supply sufficient electrostatic repulsion between the micelles to keep them sterically stable. TEM morphology analysis revealed that the structure of BCA-FS was spherical and homogeneous (Figure 2). The water-insoluble drug could remain in the core, which was composed of hydrophobic segments and surrounded by a hydrophilic chain. Hence, the mixed micelles could significantly increase the solubility of BCA, reaching 5.0 mg/mL, an increase of ~10 thousand times. The BCA drug-loading and EE were 5.88%±0.76% and 93.67%±0.98%, respectively. These results show that a micelle system comprising F127 and S630 (1:1) can effectively deliver BCA, thereby improving its bioavailability (Figure 3).

Figure 2 Structural representation and transmission electron microscopy image of BCA-loaded F127/S630 mixed polymeric micelles.
Abbreviations: BCA, biochanin A; BCA-FS, BCA-loaded micelles with Pluronic F127 and Plasdone S630.

Figure 3 Size and zeta potential of BCA-FS.
Notes: (A) Size distribution spectrum of BCA-FS and (B) zeta potential spectrum of BCA-FS.
Abbreviation: BCA-FS, biochanin A-loaded micelles with Pluronic F127 and Plasdone S630.

In vitro drug release

BCA and BCA-FS release in vitro was investigated using the dialysis method under sink conditions. The plot of accumulated release rate versus time is shown in Figure 4. Free BCA and BCA-FS micelles showed different dissolution behaviors in PBS. Free BCA was released rapidly, and ~66.84%±6.68% of BCA had been released within the initial 8 h. Compared with free BCA, the amount released from BCA-FS micelles showed the relative burst drug release of BCA at the initial stage, followed by a slow release phase, which achieved 36.09%±5.41% at 8 h and 72.86%±7.28% at 72 h. The BCA burst release from the mixed micelles may be a result of rapid disruption of the micellar system due to cohesion, a higher concentration gradient, and sink conditions in the system.28 BCA-FS showed a better sustained-release property than BCA.

Figure 4 In vitro drug release profile of BCA-FS micelles and free BCA. Release medium, phosphate-buffered saline (pH 6.8) with 0.5% (w/v) Tween 80; n=3.
Abbreviation: BCA-FS, biochanin A-loaded micelles with Pluronic F127 and Plasdone S630.

Transport in Caco-2 cell monolayers

The Caco-2 cell culture model is recognized by the US Food and Drug Administration as a viable model for human intestinal absorption. In this study, BCA and BCA-FS transport in both AP-to-BL and BL-to-AP directions was investigated. TEER values (>400 Ω/cm2) were measured before and after transport experiments to ensure that the Caco-2 cell-seeded inserts were integrated, and there was no toxicity to the cells. As shown in Figure 5 and Table 1, for AP–BL transport, BCA-FS significantly increased the absorption of BCA (P<0.05), and the apparent permeability (Papp) value was 4.33±0.27×10−6 cm/s, which is higher than that of the BCA group (2.81±0.26×10−6 cm/s). This may be due primarily to the characteristics of Pluronic F127, which incorporates into membranes followed by subsequent translocation into cells, affecting various cellular functions. BCA transport from the BL to the AP side displayed no significant differences between the experimental groups. The BCA and BCA-FS Papp values were 4.58±0.33×10−6 and 4.13±0.12×10−6 cm/s, respectively. BCA has been considered a P-glycoprotein inhibitor,29,30 and the potential inhibition mechanism may be a direct interaction, with BCA-stimulating P-gp ATPase.31,32 This could explain the relatively low Papp value. The mixed micelles system decreased the PappBL–AP, but not significantly. The efflux ratio was reduced from 1.63 to 0.96, a remarkable difference between BCA and BCA-FS. This may be attributable to the ability of BCA-FS to increase intestinal absorption of BCA. The mechanism underlying this phenomenon may be directly related to the inclusion of the two excipients.

Table 1 Permeability and efflux ratios
Notes: Absorption permeability was expressed as AP-to-BL, while secretory permeability was presented as BL-to-AP. The efflux ratio was Papp(BL–AP)/Papp(AP–BL). Data are expressed as mean ± standard deviation (n=3). *P<0.05 versus BCA group.
Abbreviations: AP, apical; BCA, biochanin A; BCA-FS, BCA-loaded micelles with Pluronic F127 and S630; BL, basolateral; Papp, apparent permeability.

Figure 5 Permeability of BCA and BCA-FS across the Caco-2 cell monolayer after a 4-h incubation at 37°C.
Notes: Data are expressed as mean ± standard deviation (n=3). *P<0.05 versus BCA group.
Abbreviations: AP, apical; BCA, biochanin A; BL, basolateral; BCA-FS, BCA-loaded micelles with Pluronic F127 and S630; Papp, apparent permeability.

Pharmacokinetic studies

BCA mean plasma concentration–time curves in rats after oral administration of 50 mg/kg BCA and BCA-FS micelles (n=6) are shown in Figure 6. A non-compartment model was used to calculate the pharmacokinetic parameters; the main parameters are summarized in Table 2.

Table 2 Main BCA pharmacokinetic parameters in rats following a 50-mg/kg oral administration of BCA and BCA-FS
Notes: Values are expressed as mean ± standard deviation (n=6). *P<0.01, **P<0.05, statistical significance versus the BCA group.
Abbreviations: AUC, area under the concentration–time curve; BCA, biochanin A; BCA-FS, BCA-loaded micelles with Pluronic F127 and S630; Cmax, maximum plasma concentration; MRT, mean residence time; Tmax, time to maximum plasma concentration; t1/2, half-life.

Figure 6 BCA plasma concentration–time curve in rats following an oral administration of BCA and BCA-FS (50 mg/kg).
Note: Values are expressed as mean ± standard deviation (n=6).
Abbreviations: BCA, biochanin A; BCA-FS, BCA-loaded micelles with Pluronic F127 and Plasdone S630.

There was a significant difference in Cmax, Tmax, and AUC0–t between the two groups. BCA treatment yielded a Cmax of 0.171±0.09 mg/L, which was significantly lower than the BCA-FS Cmax value of 0.785±0.218 mg/L (P<0.01). BCA-FS had a significantly higher AUC0–t of 1.133±0.315 mg/L/h versus 0.524±0.239 mg/L/h for BCA (P<0.01), an ~2.2-fold increase in apparent bioavailability. Tmax for BCA-FS was 0.458 h, shorter than the Tmax of 2.4 h for BCA, indicating that BCA-FS was rapidly digested and the BCA was absorbed upon entering the gastrointestinal tract. The t1/2 values for BCA and BCA-FS were 0.987±0.339 and 1.078±0.311 h, respectively, showing a slightly sustained but not significant release effect in the micelles groups.

In vivo bioavailability testing further confirmed that BCA-FS increased the plasma concentration of BCA and improved its bioavailability, perhaps because of the following reasons: 1) The F127 and S630 formulation has a small diameter and large dispersion. The particle size and surface properties further affect absorption. 2) Surfactants increase intestinal epithelial permeability by disturbing the cell membrane and reversibly opening the tight junction of intestinal epithelial cells. 3) The sustained release property of BCA-FS can achieve a longer retention time in vivo. In addition, the slight P-gp inhibition and the dosage of BCA-FS could also contribute to the improvement in bioavailability.33

Gastrointestinal safety assay

We evaluated the gastrointestinal toxicity of BCA-FS in rats after intragastric administration.34 As shown in Figure 7, the stomach wall and intestinal tract showed no pathological changes after BCA treatment, and the intestinal layer possesses an explicit structure. In the BCA-FS group, the gastric wall surface appeared slightly keratinized and the intestinal villi were shortened and appeared relatively loose; there were no inflammatory cell infiltrations or cellular morphological variations. These results suggest that BCA crossed the gastrointestinal membrane easily when the drug was delivered by the micelle mixture. Our results show that BCA-FS may stimulate the intestine mildly, but no toxicity was apparent.

Figure 7 Gastrointestinal safety assay.
Notes: (A) Stomach tissue following BCA treatment; (B) intestinal tissue following BCA treatment; (C) stomach tissue following BCA-FS treatment; and (D) intestinal tissue following BCA-FS treatment.
Abbreviations: BCA, biochanin A; BCA-FS, BCA-loaded micelles with Pluronic F127 and Plasdone S630.

Conclusion

In this study, we showed the suitability of BCA-FS as a micelle formulation for systemic delivery of BCA. BCA-FS was designed to enhance the solubility and oral absorption of BCA and showed better physical characteristics and a sustained release profile in vitro. BCA permeability across a Caco-2 cell monolayer was enhanced, and pharmacokinetic experiments in SD rats showed that the relative bioavailability of BCA-FS (AUC0–t) was 216% greater than that of raw BCA. H&E staining revealed no acute toxicity to the gastrointestinal organs. Our findings suggest that this system, comprising Pluronic F127 and Plasdone S630, could be a promising carrier for improving the poor aqueous solubility and bioavailability of BCA.

Acknowledgments

The authors greatly acknowledge Ting Liu, Jian Hou, and Dengjie Wang for their help in pharmacokinetic studies. This work was supported by the Fundamental Research Funds for the Central Universities (021414380220) and National Natural Science Foundation of China (81403121).

Disclosure

The authors report no conflicts of interest in this work.


References

1.

Szliszka E, Czuba ZP, Mertas A, Paradysz A, Krol W. The dietary isoflavone biochanin-A sensitizes prostate cancer cells to TRAIL-induced apoptosis. Urol Oncol. 2013;31(3):331–342.

2.

Chen J, Ge B, Wang Y, Ye Y, Zeng S, Huang Z. Biochanin A promotes proliferation that involves a feedback loop of microRNA-375 and estrogen receptor alpha in breast cancer cells. Cell Physiol Biochem. 2015;35(2):639–646.

3.

Bhardwaj V, Tadinada SM, Jain A, et al. Biochanin A reduces pancreatic cancer survival and progression. Anticancer Drugs. 2014;25(3):296–302.

4.

Barnes S. The chemopreventive properties of soy isoflavonoids in animal models of breast cancer. Breast Cancer Res Treat. 1997;46(2–3):169–179.

5.

Kole L, Giri B, Manna SK, Pal B, Ghosh S. Biochanin-A, an isoflavone, showed anti-proliferative and anti-inflammatory activities through the inhibition of iNOS expression, p38-MAPK and ATF-2 phosphorylation and blocking NFkappaB nuclear translocation. Eur J Pharmacol. 2011;653(1–3):8–15.

6.

Sithisarn P, Michaelis M, Schubert-Zsilavecz M, Cinatl J Jr. Differential antiviral and anti-inflammatory mechanisms of the flavonoids biochanin A and baicalein in H5N1 influenza A virus-infected cells. Antiviral Res. 2013;97(1):41–48.

7.

Lee KH, Choi EM. Biochanin A stimulates osteoblastic differentiation and inhibits hydrogen peroxide-induced production of inflammatory mediators in MC3T3-E1 cells. Biol Pharm Bull. 2005;28(10):1948–1953.

8.

Nikander E, Metsä-Heikkilä M, Ylikorkala O, Tiitinen A. Effects of phytoestrogens on bone turnover in postmenopausal women with a history of breast cancer. J Clin Endocrinol Metab. 2004;89(3):1207–1212.

9.

Harini R, Ezhumalai M, Pugalendi KV. Antihyperglycemic effect of biochanin A, a soy isoflavone, on streptozotocin-diabetic rats. Eur J Pharmacol. 2012;676(1–3):89–94.

10.

Jain A, Lai JC, Bhushan A. Biochanin A inhibits endothelial cell functions and proangiogenic pathways: implications in glioma therapy. Anticancer Drugs. 2015;26(3):323–330.

11.

Moon YJ, Sagawa K, Frederick K, Zhang S, Morris ME. Pharmacokinetics and bioavailability of the isoflavone biochanin A in rats. AAPS J. 2006;8(3):E433–E442.

12.

Wang Q, Cheng H, Zhou K, et al. Nanostructured lipid carriers as a delivery system of biochanin A. Drug Deliv. 2013;20(8):331–337.

13.

Wang L, Luo Q, Lin T, et al. PEGylated nanostructured lipid carriers (PEG-NLC) as a novel drug delivery system for biochanin A. Drug Dev Ind Pharm. 2015;41(7):1204–1212.

14.

Attia ABE, Zhan YO, Hedrick JL, et al. Mixed micelles self-assembled from block copolymers for drug delivery. Curr Opin Colloid Interface Sci. 2011;16(3):182–194.

15.

Ling G, Zhang P, Zhang W, et al. Development of novel self-assembled DS-PLGA hybrid nanoparticles for improving oral bioavailability of vincristine sulfate by P-gp inhibition. J Control Release. 2010;148(2):241–248.

16.

Song Y, Tian Q, Huang Z, et al. Self-assembled micelles of novel amphiphilic copolymer cholesterol-coupled F68 containing cabazitaxel as a drug delivery system. Int J Nanomedicine. 2014;9(1):2307–2317.

17.

Zhang W, Shi Y, Chen Y, Ye J, Sha X, Fang X. Multifunctional Pluronic P123/F127 mixed polymeric micelles loaded with paclitaxel for the treatment of multidrug resistant tumors. Biomaterials. 2011;32(11):2894–2906.

18.

Zhang W, Shi Y, Chen Y, Hao J, Sha X, Fang X. The potential of Pluronic polymeric micelles encapsulated with paclitaxel for the treatment of melanoma using subcutaneous and pulmonary metastatic mice models. Biomaterials. 2011;32(25):5934–5944.

19.

Wei Z, Yuan S, Chen Y, et al. Enhanced antitumor efficacy by Paclitaxel-loaded Pluronic P123/F127 mixed micelles against non-small cell lung cancer based on passive tumor targeting and modulation of drug resistance. Eur J Pharm Biopharm. 2010;75(3):341–353.

20.

Mitra A, Li L, Marsac P, Marks B, Zhen L, Brown C. Impact of polymer type on bioperformance and physical stability of hot melt extruded formulations of a poorly water soluble drug. Int J Pharm. 2016;505(1–2):107–114.

21.

Maniruzzaman M, Rana MM, Boateng JS, Mitchell JC, Douroumis D. Dissolution enhancement of poorly water-soluble APIs processed by hot-melt extrusion using hydrophilic polymers. Drug Dev Ind Pharm. 2013;39(2):218–227.

22.

Ghebremeskel A, Vemavarapu C, Lodaya M. Use of surfactants as plasticizers in preparing solid dispersions of poorly soluble API: selection of polymer–surfactant combinations using solubility parameters and testing the processability. Int J Pharm. 2007;328(2):119–129.

23.

Hou J, Wang J, Sun E, et al. Preparation and evaluation of icariside II-loaded binary mixed micelles using Solutol® HS15 and Pluronic F127 as carriers. Drug Deliv. 2016;23(9):1–24.

24.

Jin X, Zhang ZH, Sun E, et al. Enhanced oral absorption of 20(S)-protopanaxadiol by self-assembled liquid crystalline nanoparticles containing piperine: in vitro and in vivo studies. Int J Nanomedicine. 2013;8(1):641–652.

25.

Elgart A, Cherniakov I, Aldouby Y, Domb AJ, Hoffman A. Improved oral bioavailability of BCS class 2 compounds by self nano-emulsifying drug delivery systems (SNEDDS): the underlying mechanisms for amiodarone and talinolol. Pharm Res. 2013;30(12):3029–3044.

26.

Agüeros M, Zabaleta V, Espuelas S, Campanero MA, Irache JM. Increased oral bioavailability of paclitaxel by its encapsulation through complex formation with cyclodextrins in poly(anhydride) nanoparticles. J Control Release. 2010;145(1):2–8.

27.

Dahmani FZ, Yang H, Zhou J, Yao J, Zhang T, Zhang Q. Enhanced oral bioavailability of paclitaxel in pluronic/LHR mixed polymeric micelles: preparation, in vitro and in vivo evaluation. Eur J Pharm Sci. 2012;47(1):179–189.

28.

Butt AM, Amin MCIM, Katas H, Sarisuta N, Witoonsaridsilp W, Benjakul R. In vitro characterization of pluronic F127 and D-α-tocopheryl polyethylene glycol 1000 succinate mixed micelles as nanocarriers for targeted anticancer-drug delivery. J Nanomater. 2012;2012(2):11.

29.

Zhang S, Sagawa K, Arnold RD, Tseng E, Wang X, Morris ME. Interactions between the flavonoid biochanin A and P-glycoprotein substrates in rats: in vitro and in vivo. J Pharm Sci. 2010;99(1):430–441.

30.

Peng SX, Ritchie DM, Cousineau M, Danser E, Dewire R, Floden J. Altered oral bioavailability and pharmacokinetics of P-glycoprotein substrates by coadministration of biochanin A. J Pharm Sci. 2006;95(9):1984–1993.

31.

Zhang S, Morris ME. Effects of the flavonoids biochanin A, morin, phloretin, and silymarin on P-glycoprotein-mediated transport. J Pharmacol Exp Ther. 2003;304(3):1258–1267.

32.

Aller SG. Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding. Science. 2010;323(5922):1718–1722.

33.

Li L, Ye BT. Preparation and properties of mixed micelles made of pluronic polymer and PEG-PE. J Colloid Interface Sci. 2008;317(1):326–331.

34.

Hajrezaie M, Salehen N, Karimian H, et al. Biochanin a gastroprotective effects in ethanol-induced gastric mucosal ulceration in rats. PLoS One. 2015;10(3):e0121529.

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]