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Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
Authors Kaushik A, Yndart A, Jayant RD, Sagar V, Atluri V, Bhansali S, Nair M
Received 8 October 2014
Accepted for publication 13 November 2014
Published 19 January 2015 Volume 2015:10(1) Pages 677—685
DOI https://doi.org/10.2147/IJN.S75514
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Editor who approved publication: Prof. Dr. Thomas J. Webster
Ajeet Kaushik,1 Adriana Yndart,1 Rahul Dev Jayant,1 Vidya Sagar,1 Venkata Atluri,1 Shekhar Bhansali,2 Madhavan Nair1
1Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2BioMEMS Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA
Abstract: A novel electrochemical sensing method was devised for the first time to detect plasma cortisol, a potential psychological stress biomarker, in human immunodeficiency virus (HIV)-positive subjects. A miniaturized potentiostat (reconfigured LMP91000 chip) interfaced with a microfluidic manifold containing a cortisol immunosensor was employed to demonstrate electrochemical cortisol sensing. This fully integrated and optimized electrochemical sensing device exhibited a wide cortisol-detection range from 10 pg/mL to 500 ng/mL, a low detection limit of 10 pg/mL, and sensitivity of 5.8 µA (pg mL)-1, with a regression coefficient of 0.995. This cortisol-selective sensing system was employed to estimate plasma cortisol in ten samples from HIV patients. The electrochemical cortisol-sensing performance was validated using an enzyme-linked immunosorbent assay technique. The results obtained using both methodologies were comparable within 2%–5% variation. The information related to psychological stress of HIV patients can be correlated with disease-progression parameters to optimize diagnosis, therapeutic, and personalized health monitoring.
Keywords: psychological stress, personalized health care, cortisol, HIV, electrochemical immunosensing, miniaturized sensing device
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