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EGCG Regulates Cell Apoptosis of Human Umbilical Vein Endothelial Cells Grown on 316L Stainless Steel for Stent Implantation

Authors Wang J, Wang Y, Zhao Y, Zhao J, Zhang B, Xu K

Received 9 December 2020

Accepted for publication 21 January 2021

Published 11 February 2021 Volume 2021:15 Pages 493—499

DOI https://doi.org/10.2147/DDDT.S296548

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Anastasios Lymperopoulos


Jinpeng Wang,1 Yue Wang,2 Yuyi Zhao,2 Jinbin Zhao,2 Beilin Zhang,3 Kun Xu2,4

1Department of Cardiology, The Second Hospital of Jilin University, Changchun, 130041, People’s Republic of China; 2Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, 130021, People’s Republic of China; 3Department of Physiology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, People’s Republic of China; 4Jilin Engineering Research Center of Public Health Detection, Changchun, 130021, People’s Republic of China

Correspondence: Kun Xu
Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, 130021, People’s Republic of China
Email xukun@jlu.edu.cn

Background: Nowadays, medical grade 316L stainless steel (316L SS) is being widely used for intravascular stents, and the drug-eluting stent (DES) system is able to significantly reduce the occurrences of in-stent restenosis. But the drugs and the polymer coating used in DES potentially induce the forming of late stent thrombosis. In order to reduce the occurrence of ISR after stent implantation, the development of novel drugs for DESs is urgently needed.
Methods: This study aimed to investigate the potential mechanisms of epigallocatechin-3-gallate (EGCG) on human umbilical vein endothelial cells (HUVEC) grown on 316L stainless steel (316L SS) using flow cytometry and Q-PCR methods.
Results: Our results showed that EGCG (12.5, 25, 50, 100 μmol/L) significantly inhibited HUVEC proliferation. Flow cytometry analysis indicated that EGCG (25, 50, 100 μmol/L) induced apoptosis. Moreover, qRT-PCRrevealed that genes associated with cell apoptosis (caspase-3, 8, 9, Fas) and autophagy (Atg 5, Atg 7, Atg 12) were up-regulated after EGCG treatment.
Conclusion: These findings indicate that EGCG possesses chemo preventive potential in stent coating which may serve as a novel new drug for stent implantation.

Keywords: EGCG, 316 stainless steel, HUVECs, apoptosis

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