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Effects Of Triptolide On Tooth Movement And Root Resorption In Rats

Authors Yang F, Wang XX, Ma D, Cui Q, Zheng DH, Liu XC, Zhang J

Received 31 May 2019

Accepted for publication 11 November 2019

Published 25 November 2019 Volume 2019:13 Pages 3963—3975

DOI https://doi.org/10.2147/DDDT.S217936

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 2

Editor who approved publication: Dr Georgios D. Panos


Fan Yang,1,2 Xu Xia Wang,1,3 Dan Ma,1,2 Qun Cui,1,2 De Hua Zheng,1,2 Xiao Can Liu,1,2 Jun Zhang1,2

1Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 2Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan, Shandong Province, People’s Republic of China

Correspondence: Jun Zhang
Department of Orthodontics, School of Stomatology, Shandong University, 44 Wenhua West Road, Jinan 250012, People’s Republic of China
Tel +86 139 5310 9816
Fax +86-531-88565657
Email zhangj@sdu.edu.cn

Purpose: The aim of this study was to investigate the effects of triptolide on the tooth movement and root resorption in rats during orthodontic treatment.
Material and methods: A total of 48 male Wistar rats were divided into three groups of 16 each. The right maxillary first molars of rats were drawn mesially by closed coil nickel–titanium spring with a force of 50 g. The two experimental groups received intraperitoneal injections of triptolide for 14 days at a dose of 15 μg/kg/day and 30 μg/kg/day, respectively. The control group received vehicle injections. After 14 days, the rats were humanely killed. The amount of tooth movement was measured. Eight rats from each group were randomly chosen for analysis of the percentage of root resorption area by scanning electron microscopy. For the remaining eight rats in each group, the H&E staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry analysis were performed.
Results: The amount of tooth movement and the ratio of root resorption area were significantly decreased in the triptolide-treated rats. The number of TRAP-positive cells was significantly lower in triptolide-treated groups. Moreover, the expression of nuclear factor kappa B ligand (RANKL) was reduced. In contrast, the expression of osteoprotegerin was significantly up-regulated. In the tension side, the expressions of runt-related transcription factor 2 and osteocalcin were significantly enhanced by triptolide injection.
Conclusion: Triptolide injection could arrest orthodontic tooth movement and reduce root resorption in rats via inhibition of osteoclastogenesis. In addition, triptolide may exert a positive effect on osteoblastogenesis.

Keywords: triptolide, osteoclast, scanning electron microscope, tooth movement, bone formation


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