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Effects of quercetin, sitagliptin alone or in combination in testicular toxicity induced by doxorubicin in rats

Authors Ahmed ZA, Abtar AN, Othman HH, Aziz TA

Received 5 July 2019

Accepted for publication 30 August 2019

Published 20 September 2019 Volume 2019:13 Pages 3321—3329

DOI https://doi.org/10.2147/DDDT.S222127

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Palas Chanda

Peer reviewer comments 4

Editor who approved publication: Dr Anastasios Lymperopoulos


Video abstract presented by Tavga Ahmed Aziz

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Zheen Aorahman Ahmed,1 Aso Nihad Abtar,1 Hemn Hassan Othman,2 Tavga Ahmed Aziz1

1Department of Pharmacology and Toxicology, College of Pharmacy, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq; 2Department of Anatomy and Pathology, College of Veterinary Medicine, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq

Correspondence: Zheen Aorahman Ahmed; Tavga Ahmed Aziz
Department of Pharmacology and Toxicology, College of Pharmacy, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq
Tel +964 770 972 4959; +964 770 152 3455
Email zheen.ahmed@univsul.edu.iq; tavga.aziz@univsul.edu.iq

Objective: This study aimed to evaluate the effect of quercetin and/or sitagliptin on testicular damage induced by doxorubicin (DOX).
Methodology: Twenty-five male Wistar rats, weighing 240±20 g, were randomly divided into five groups as follows: a negative control group; that was treated with 1 mL of 0.9% sodium chloride; a DOX-treated group received Intraperitoneal (I.P.) DOX injection (3 mg/kg); a group treated with quercetin 80 mg/kg + sitagliptin 10 mg/kg + DOX; a group treated with quercetin 80 mg/kg + DOX; and a group treated with sitagliptin 10 mg/kg+ DOX. All treatment were given orally daily for 21 days with I.P. DOX 3 mg/kg injection for the treatment groups at days 8, 10, 12, 15, 17, and 19. On day 22, blood was collected for analysis of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glutathione peroxidase (GPx), and total antioxidant capacity (TAOC). The testes were also removed and sent for histopathological examination.
Results: The study revealed that the combination of quercetin with sitagliptin produced a significant increase in testosterone and FSH levels with a non-significant increase in LH level. This combination also non-significantly decreased the level of ALP and LDH and restored the GPx level with enhancing TAOC.
Conclusion: The results suggest quercetin/sitagliptin combination as a promising therapeutic modality for attenuation of DOX-induced testicular toxicity in rats, and the main mechanism involved in such effect could be due to the antioxidant and anti-inflammatory properties of both agents.

Keywords: doxorubicin, quercetin, sitagliptin, testicular damage

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