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Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles

Authors Wang C, Gao X, Wang F, Guan W, Dou H, Xu G

Received 24 September 2020

Accepted for publication 24 October 2020

Published 20 November 2020 Volume 2020:15 Pages 9255—9264

DOI https://doi.org/10.2147/IJN.S283430

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Farooq A. Shiekh


Chenglong Wang,1 Xuzhu Gao,1 Fanchen Wang,1 Wencai Guan,1 Hongjing Dou,2 Guoxiong Xu1

1Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China

Correspondence: Guoxiong Xu
Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, 1508 Longhang Road, Shanghai 201508, People’s Republic of China
Tel +86-21-34189990
Fax +86-21-57039502
Email guoxiong.xu@fudan.edu.cn
Hongjing Dou
The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People’s Republic of China
Tel +86-21-34202956
Fax +86-21-34202745
Email hjdou@sjtu.edu.cn

Introduction: Chemoresistance leads to chemotherapy failure in patients with cancer. Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease. Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption. Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.
Methods: A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.
Results: Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells. Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.
Conclusion: Starvation therapy can be used as a new method to reverse drug resistance in cancer.

Keywords: cancer drug-resistance, P-glycoprotein, starvation therapy, nanoparticles, resistance reversal

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