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Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells

Authors Xia G, Chen B, Ding J, Gao C, Lu H, Shao Z, Gao F, Wang X

Published 8 September 2011 Volume 2011:6 Pages 1921—1927

DOI https://doi.org/10.2147/IJN.S24078

Review by Single-blind

Peer reviewer comments 3

Guohua Xia1,2, Baoan Chen1,2, Jiahua Ding1,2, Chong Gao1,2, Huixia Lu1,2, Zeye Shao1,2, Feng Gao1,2, Xuemei Wang2,3
1Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of China; 2Faculty of Oncology, Medical School, Southeast University, Nanjing, People’s Republic of China; 3National Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, People’s Republic of China

Abstract: This study aims to evaluate the potential benefit of combination therapy of 2-methoxyestradiol (2ME) and magnetic nanoparticles of Fe3O4 (MNPs- Fe3O4) on myelodysplastic syndrome (MDS) SKM-1 cells and its underlying mechanisms. The effect of the unique properties of tetraheptylammonium-capped MNPs- Fe3O4 with 2ME on cytotoxicity was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay. Cell-cycle distribution and apoptosis were assessed by flow cytometry. The expression of cell-cycle marker protein was measured by Western blotting. Growth inhibition rate of SKM-1 cells treated with the 2ME-loaded MNPs- Fe3O4 was enhanced when compared with 2ME alone. 2ME led to an increase of caspase-3 expression, followed by apoptosis, which was significantly increased when combined with an MNPs- Fe3O4 carrier. Moreover, the copolymer of 2ME with MNPs- Fe3O4 blocked a nearly two-fold increase in SKM-1 cells located in G2/M phase than in 2ME alone, which may be associated with an accompanying increase of p21 as well as a decrease in cyclin B1 and cdc2 expression, but there was no obvious difference between the MNPs- Fe3O4 and control group. These findings suggest that the unique properties of MNPs- Fe3O4 as a carrier for 2ME, a new anticancer agent currently in clinical trials, may be a logical strategy to enhance the therapeutic activity of MDS.

Keywords: MDS, MNPs- Fe3O4, SKM-1 cell, cell cycle

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