Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p
Authors Shan Q, Qu F, Yang W, Chen N
Received 13 February 2020
Accepted for publication 21 April 2020
Published 15 June 2020 Volume 2020:12 Pages 4561—4571
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Eileen O'Reilly
Qiuli Shan,1 Fan Qu,1 Weiping Yang,2 Ningning Chen1
1College of Biological Science and Technology, University of Jinan, Jinan 250022, People’s Republic of China; 2Department of Thyroid Breast Surgery, Qingdao Chengyang People’s Hospital, Qingdao, People’s Republic of China
Correspondence: Qiuli Shan
College of Biological Science and Technology, University of Jinan, No. 336, Nanxinzhuan West Road, Jinan, Shandong Province 250022, People’s Republic of China
Objective: To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p.
Methods: Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People’s Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were collected during the operation with the permission of the patients. The expressions of LINC00657 and miR-590-3p were detected in breast carcinoma cells and tissues. The breast carcinoma cells were transfected and their proliferation, migration, invasion and apoptosis were detected.
Results: LINC00657 was highly expressed in breast carcinoma tissues, while miR-590-3p was reduced (P< 0.05). The proliferation, invasion and migration of cells transfected with si-LINC00657 or miR-590-3p-mimics were significantly inhibited, and the apoptosis rate increased, resulting in the up-regulation of the expressions of apoptosis-related proteins Bax and Caspase-3 and the reduction of Bcl-2 (P< 0.05). After si-LINC00657 or miR-590-3p-mimics, the level of GOLPH3 decreased. Through double luciferase report and RIP experiment, it was confirmed that LINC00657 could act as a sponge of miR-590-3p to negatively regulate its expression. After correlation analysis, it was concluded that there was a negative correlation between LINC00657 and miR-590-3p. Rescue experiments concluded that co-transfection of si-LINC00657+miR-590-3P-inhibitor could reverse the inhibitory action of si-LINC00657 on breast carcinoma cells.
Conclusion: LINC00657 can participate in the biological behavior process of breast carcinoma by regulating miR-590-3p/GOLPH3 signal.
Keywords: LINC00657, miR-590-3p, GOLPH3, breast carcinoma, apoptosis
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]