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Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p

Authors Shan Q, Qu F, Yang W, Chen N

Received 13 February 2020

Accepted for publication 21 April 2020

Published 15 June 2020 Volume 2020:12 Pages 4561—4571

DOI https://doi.org/10.2147/CMAR.S249576

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Qiuli Shan,1 Fan Qu,1 Weiping Yang,2 Ningning Chen1

1College of Biological Science and Technology, University of Jinan, Jinan 250022, People’s Republic of China; 2Department of Thyroid Breast Surgery, Qingdao Chengyang People’s Hospital, Qingdao, People’s Republic of China

Correspondence: Qiuli Shan
College of Biological Science and Technology, University of Jinan, No. 336, Nanxinzhuan West Road, Jinan, Shandong Province 250022, People’s Republic of China
Tel +86-18753151252
Email bio_shanql@ujn.edu.cn

Objective: To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p.
Methods: Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People’s Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were collected during the operation with the permission of the patients. The expressions of LINC00657 and miR-590-3p were detected in breast carcinoma cells and tissues. The breast carcinoma cells were transfected and their proliferation, migration, invasion and apoptosis were detected.
Results: LINC00657 was highly expressed in breast carcinoma tissues, while miR-590-3p was reduced (P< 0.05). The proliferation, invasion and migration of cells transfected with si-LINC00657 or miR-590-3p-mimics were significantly inhibited, and the apoptosis rate increased, resulting in the up-regulation of the expressions of apoptosis-related proteins Bax and Caspase-3 and the reduction of Bcl-2 (P< 0.05). After si-LINC00657 or miR-590-3p-mimics, the level of GOLPH3 decreased. Through double luciferase report and RIP experiment, it was confirmed that LINC00657 could act as a sponge of miR-590-3p to negatively regulate its expression. After correlation analysis, it was concluded that there was a negative correlation between LINC00657 and miR-590-3p. Rescue experiments concluded that co-transfection of si-LINC00657+miR-590-3P-inhibitor could reverse the inhibitory action of si-LINC00657 on breast carcinoma cells.
Conclusion: LINC00657 can participate in the biological behavior process of breast carcinoma by regulating miR-590-3p/GOLPH3 signal.

Keywords: LINC00657, miR-590-3p, GOLPH3, breast carcinoma, apoptosis


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