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Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells

Authors Li X, Pei B, Wang H, Tang C, Zhu W, Jin F

Received 19 December 2017

Accepted for publication 5 March 2018

Published 6 June 2018 Volume 2018:11 Pages 3359—3368

DOI https://doi.org/10.2147/OTT.S160313

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Justinn Cochran

Peer reviewer comments 3

Editor who approved publication: Dr Ingrid Espinoza


Xueqing Li,1,* Bing Pei,2,* Hui Wang,1 Chenghui Tang,1 Wei Zhu,3,4 Feng Jin5

1Department of General Surgery, The Fifth People’s Hospital of Shanghai Fudan University, Shanghai, People’s Republic of China; 2Clinical Laboratory, Suqian First Hospital, Suqian, People’s Republic of China; 3Department of Anesthesia, Jiangsu People’s Hospital, Nanjing, People’s Republic of China; 4Department of Anesthesia, Suqian First Hospital, Suqian, People’s Republic of China; 5Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, People’s Republic of China

*These authors contributed equally to this work

Objectives: Based on the functionality of AQP-5 characterized in various physiological processes, our study aimed to investigate the effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells.
Materials and methods: The expression levels of AQP-5 mRNA in different experimental groups were measured by reverse transcription PCR. The chemosensitivity of the cells to adriamycin (ADR) was detected by a CCK-8 kit. Cell invasion, migration, and apoptosis were assessed. Western blot was used to detect the expression levels of AQP-5, drug resistance-related protein, and apoptosis-related protein.
Results: The expression level of AQP-5 in MCF-7/ADR cells was significantly reduced by AQP-5 siRNA transfection. The invasion and migration were significantly reduced in MCF-7/ADR after AQP-5 siRNA interference. AQP-5 silencing significantly increased the chemosensitivity of MCF-7/ADR cells to ADR and activated caspase-dependent apoptosis in MCF-7/ADR cells. AQP-5 silencing also decreased the expression levels of drug resistance-related proteins (P-gp and GST-Π).
Conclusion: The inhibition of AQP-5 expression may reverse the drug resistance and enhance the chemosensitivity of breast cancer cells.

Keywords: aquaporin 5, breast cancer, ADR, chemosensitivity, apoptosis

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