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Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology

Authors Zhang G, Nie M, Webster TJ, Zhang Q, Fan W

Received 23 December 2018

Accepted for publication 6 May 2019

Published 2 July 2019 Volume 2019:14 Pages 4755—4765

DOI https://doi.org/10.2147/IJN.S199306

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Guangcheng Zhang,1 Mingjun Nie,2 Thomas J Webster,3 Qing Zhang,2 Weimin Fan1

1Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China; 3Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA

Background: Many techniques and methods have been used clinically to relieve pain from cartilage repair, but the long-term effect is still unsatisfactory.
Purpose: The objective of this study was to form an artificial chondroid tissue gene enhanced tissue engineering system to repair cartilage defects via nanosized liposomes.
Methods: Cationic nanosized liposomes were prepared and characterized using transmission electron microscope (TEM) and dynamic laser light scattering (DLS). The rat mesenchymal stem cells (rMSCs) were isolated, cultivated, and induced by SRY (Sex-Determining Region Y)-Box 9 (Sox9) via cationic nanosized liposomes. The induced rMSCs were mixed with a thermo-sensitive chitosan hydrogel and subcutaneously injected into the nude mice. Finally, the newly-formed chondroid tissue obtained in the injection parts, and the transparent parts were detected by HE, collagen II, and safranin O.
Results: It was found that the presently prepared cationic nanosized liposomes had the diameter of 85.76±3.48 nm and the zeta potential of 15.76±2.1 mV. The isolated rMSCs proliferation was fibroblast-like, with a cultivated confluence of 90% confluence in 5–8 days, and stained positive for CD29 and CD44 while negative for CD34 and CD45. After transfection with cationic nanosized liposomes, we observed changes of cellular morphology and a higher expression of SOX9 compared with control groups, which indicated that rMSCs could differentiate into chondrocyte in vitro. By mixing transfected rMSCs with the thermo-sensitive hydrogel of chitosan in nude mice, chondroid tissue was successfully obtained, demonstrating that rMSCs can differentiate into chondrogenic cells in vivo.
Conclusion: This study explored new ways to improve the quality of tissue engineered cartilage, thus accelerating clinical transformation and reducing patient pain.

Keywords: Sox9, chondrogenesis, gene enhanced tissue engineering, transfection, chondroid



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