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E2F1 induces LSINCT5 transcriptional activity and promotes gastric cancer progression by affecting the epithelial-mesenchymal transition

Authors Qi P, Lin W, Zhang M, Huang D, Ni S, Zhu X, Bai Q, Sheng W, Du X, Zhou X

Received 19 April 2018

Accepted for publication 14 June 2018

Published 10 August 2018 Volume 2018:10 Pages 2563—2571

DOI https://doi.org/10.2147/CMAR.S171652

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 2

Editor who approved publication: Dr Antonella D'Anneo


Peng Qi,1–3 Wan-run Lin,4 Meng Zhang,1–3 Dan Huang,1–3 Shu-juan Ni,1–3 Xiao-li Zhu,1–3 Qian-ming Bai,1–3 Wei-qi Sheng,1–3 Xiang Du,2 Xiao-yan Zhou1–3

1Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China; 2Institute of Pathology, Fudan University, Shanghai, China; 3Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; 4Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

Background: Long noncoding RNAs (lncRNAs) have been shown to play important regulatory roles in human cancer. We previously verified that the lncRNA long stress-induced noncoding transcript 5 (LSINCT5) is overexpressed in gastric cancer (GC) cells and closely correlated with cell proliferation and patient prognosis. However, whether aberrant LSINCT5 expression has an important effect on GC progression is unclear, and the potential mechanisms remain unknown. In GC, E2F1 expression is also aberrant, but the biological functions of E2F1 are controversial, and the correlation between E2F1 and lncRNAs remains unknown.
Materials and methods: Expression of LSINCT5 was analyzed in metastatic GC tissues compared with nonmetastatic tissues using quantitative real-time PCR (qRT-PCR) assays. Gain and loss of function approaches were used to investigate the biological role of LSINCT5 in GC cell migration and invasion. A computational screen of LSINCT5 promoter was conducted to search for transcription factor-binding sites. LSINCT5 promoter activities were examined by ChIP and luciferase reporter assays. qRT-PCR and western blotting assays were performed to detect the expression of multiple EMT markers in cells in which LSINCT5 was overexpressed or knocked down.
Results: An integrated quantitative analysis revealed that LSINCT5 was significantly overexpressed in metastatic GC tissues. Forced LSINCT5 expression promoted cell migration and invasion, whereas loss of LSINCT5 function decreased cell migration and invasion. Mechanistic investigations showed that LSINCT5 is a direct transcriptional target of E2F1. Moreover, LSINCT5 overexpression was found to play an important role in the epithelial-to-mesenchymal transition by regulating the expression of E-cadherin, N-cadherin, vimentin, and matrix metalloproteinase-2.
Conclusion: These data suggest that E2F1-mediated activation of LSINCT5, a regulator of cell migration and invasion, constitute the mechanistic link between the E2F1-mediated pathway and lncRNA that regulates cell migration and invasion. Thus, LSINCT5 may be a target for new GC therapies.

Keywords: E2F1, LSINCT5, metastasis, gastric cancer

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