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E2F transcription factor 8 promotes cell proliferation via CCND1/p21 in esophageal squamous cell carcinoma

Authors Chang H, Song J, Wu J, Zhang Y

Received 20 July 2018

Accepted for publication 17 October 2018

Published 15 November 2018 Volume 2018:11 Pages 8165—8173

DOI https://doi.org/10.2147/OTT.S180938

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Sanjeev Srivastava


Huiwen Chang, Jianxiang Song, Jixiang Wu, Yajun Zhang

Department of Cardiothoracic Surgery, Yancheng Third People’s Hospital, The Affiliated Yancheng Hospital of Southeast University, Yancheng, Jiangsu 224001, PR China

Purpose: E2F transcription factor 8 (E2F8) is a novel member of the E2F family, but its function in esophageal squamous cell carcinoma (ESCC) remains unclear. This study aimed to research the function of E2F8 in ESCC.
Materials and methods: We used quantitative real-time PCR and Western blot analyses to detect the expression pattern of E2F8 in ESCC. The effects of E2F8 on proliferation were investigated by Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, and colony formation assays. We also confirmed the function of E2F8 in vivo.
Results: E2F8 expression was upregulated in ESCC, and promoted cell proliferation and influenced the expression of CCND1/p21. Downregulation of E2F8 expression inhibited cell proliferation in vivo.
Conclusion: E2F8 was identified as a new potential oncogene in ESCC.

Keywords: E2F8, cell cycle, ESCC, cell proliferation

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