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Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds

Authors John WJ Slavin, Albena Ivanisevic

Published 15 January 2008 Volume 2007:2(4) Pages 821—825



John WJ Slavin1, Albena Ivanisevic1,2

1Department of Chemistry and 2Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA

Abstract: DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localization of components using biological media.

Keywords: DNA, gold nanoparticles, restriction enzymes, surface, digestion