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Downregulation of lncRNA ANRIL suppresses growth and metastasis in human osteosarcoma cells

Authors Guan H, Mei Y, Mi Y, Li C, Sun X, Zhao X, Liu J, Cao W, Li Y, Wang Y

Received 6 April 2018

Accepted for publication 25 May 2018

Published 14 August 2018 Volume 2018:11 Pages 4893—4899

DOI https://doi.org/10.2147/OTT.S170293

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Dr Carlos E Vigil


Hongya Guan,1 Yingwu Mei,2 Yang Mi,2 Cheng Li,3 Xiaoya Sun,2 Xuefeng Zhao,2 Jia Liu,1 Wei Cao,1 Yuebai Li,2 Yisheng Wang3

1
Translational Medical Center, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, People’s Republic of China; 2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, People’s Republic of China; 3Department of Orthopedic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, People’s Republic of China

Background: This study was designed to research the potential function of lncRNA ANRIL in osteosarcoma (OS).
Materials and methods: Quantitative real-time PCR, cell counting kit-8, wound healing assay, Transwell assay, flow cytometric analysis, caspase activity analysis, and Western blot were carried out.
Results: ANRIL was remarkably upregulated in human OS tissues and cells, and knockdown of ANRIL significantly suppressed MG63 cell proliferation, migration, and invasion and promoted apoptosis. Moreover, our mechanistic research findings verified that ANRIL-influenced growth and apoptosis may be partly through regulation of caspase-3 and Bcl-2. Migration and invasion were influenced via ANRIL-mediated regulation of MTA1, TIMP-2, and E-cadherin.
Conclusion: Our finding demonstrates that ANRIL plays vital roles in OS growth and metastasis.

Keywords: osteosarcoma, ANRIL, proliferation, invasion, apoptosis, long noncoding RNA

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