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Downregulation of CD99 and upregulation of human leukocyte antigen class II promote tumor aggravation and poor survival in patients with osteosarcomas

Authors Zhou Q, Xu J, Zhao J, Zhang S, Pan W

Received 20 September 2013

Accepted for publication 18 November 2013

Published 24 March 2014 Volume 2014:7 Pages 477—484


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Quan Zhou,* Jin Xu,* Jiali Zhao, Shaoxian Zhang, Wei Pan

Department of Orthopaedics, the second Hospital of Huai'an city Affiliated to Xuzhou Medical College and the second Hospital of Huai'an city, Huai'an, People's Republic of China

*These authors contributed equally to this work

Background: CD99 is involved in the intracellular transport of human leukocyte antigen class II (HLA-II) protein. The aim of this study was to clarify the clinical value of CD99 and HLA-II expression in primary osteosarcoma.
Methods: One hundred and thirty pairs of osteosarcoma and matched noncancerous bone tissues were evaluated by immunohistochemistry for CD99 and HLA-II expression.
Results: Compared with the noncancerous bone tissues, the expression levels of CD99 (tumor versus normal: 2.96±0.09 versus 5.89±1.26, P<0.001) and HLA-II (tumor versus normal: 5.01±1.39 versus 1.92±0.06, P<0.001) proteins were respectively downregulated and upregulated in osteosarcoma tissues. CD99 and HLA-II were highly expressed in 49/130 (37.69%) and 107/130 (82.31%) of osteosarcoma tissues, respectively. In addition, the osteosarcoma patients with downregulation of CD99 and upregulation of HLA-II more frequently showed the presence of metastasis and recurrence and poor response to chemotherapy. Moreover, there was a negative correlation between CD99 and HLA-II expression in osteosarcoma tissues (r=-0.69, P=0.01). The patients with low CD99 expression correlated with poor prognosis of osteosarcoma, as opposed to HLA-II. Patients with CD99-low/HLA-II-high expression had the lowest overall and disease-free survival rates, and conjoined expression of CD99/HLA-II was an independent prognostic indicator of osteosarcoma.
Conclusion: These findings suggest for the first time that CD99 downregulation or HLA-II upregulation may be an important feature of human osteosarcoma. The combined detection of CD99/HLA-II coexpression may present a predictive and prognostic indicator in osteosarcoma.

Keywords: CD99, HLA-II, osteosarcoma, immunohistochemistry, prognosis

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