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Downregulation of long noncoding RNA HOTAIRM1 promotes monocyte/dendritic cell differentiation through competitively binding to endogenous miR-3960

Authors Xin J, Li J, Feng Y, Wang L, Zhang Y, Yang R

Received 8 October 2016

Accepted for publication 18 November 2016

Published 1 March 2017 Volume 2017:10 Pages 1307—1315

DOI https://doi.org/10.2147/OTT.S124201

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Manfred Beleut

Peer reviewer comments 3

Editor who approved publication: Dr William Cho

Jiaxuan Xin,1 Jing Li,1 Yue Feng,1 Liyang Wang,2 Yuan Zhang,1 Rongcun Yang1,3,4

1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, People’s Republic of China; 2Faculty of Medicine, University of Southampton, Southampton, UK; 3Key Laboratory of Bioactive Materials, Ministry of Education, 4Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin, People’s Republic of China

Abstract: Myeloid differentiation is controlled by a multilayered regulatory circuitry consisting of various elements, including histone modifications, transcription factors, and posttranscriptional regulators such as miRNAs, long noncoding RNAs, and circular RNAs. However, the molecular mechanism underlying this biological process remains unclear. In this study, through epigenetic profiling analysis using chromatin immunoprecipitation (ChIP) followed by sequencing (ChIP-seq), we identified an lncRNA, HOTAIRM1, with a critical role in myeloid development. Further ChIP-chip analysis showed obvious H3K4me3 and H3K27me3 histone modification peak changes in the promoter region of HOTAIRM1 during the process of monocyte to dendritic cell (DC) differentiation. In line with this observation, HOTAIRM1 RNA expression was downregulated when monocytes differentiated into DCs. Moreover, we found that HOTAIRM1 RNA was regulated by epigenetic factors such as RBBP4 and RBBP7. Mechanistically, we found that the silencing of HOTAIRM1 caused changes in the expression of several monocyte differentiation markers such as CD14 and B7H2. In addition, based on the “competing endogenous RNA” hypothesis, we discovered miR-3960 targeting both HOTAIRM1 and the DC differentiation repression gene, HOXA1, by most possibly constructing a potential competing endogenous RNA network. Increased miR-3960 expression could downregulate both of these two long RNAs and finally lead peripheral blood cells to differentiate into DCs. In summary, our study demonstrates that HOTAIRM1 competitively binds to miR-3960 and finally regulates the process of hematopoiesis, which reveals a novel regulatory mechanism of lncRNA function.

Keywords: myeloid cells, lncRNA, HOTAIRM1, miR-3960, myeloid differentiation

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