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Downregulated miR-585-3p promotes cell growth and proliferation in colon cancer by upregulating PSME3

Authors Liu C, Yang J, Wu H, Li J

Received 28 January 2019

Accepted for publication 16 July 2019

Published 14 August 2019 Volume 2019:12 Pages 6525—6534

DOI https://doi.org/10.2147/OTT.S203175

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Aruna Narula

Peer reviewer comments 3

Editor who approved publication: Professor Jianmin Xu


Chunmei Liu,1,2 Juan Yang,1,2 Han Wu,1,2 Jun Li3

1Department of Pathology, Luohe Central Hospital, Luohe 462000, People’s Republic of China; 2Department of Pathology, The First Affiliated Hospital of Luohe Medical College, Luohe 462000, People’s Republic of China; 3Nursing Department, Xiangya Hospital, Central South University, Changsha 410078, People’s Republic of China

Background: Upregulation of PSME3 and its oncogenic roles have been reported in colon cancer recently. However, the underlying mechanism of PSME3 upregulation remains unknown. Here, we explored the expression of PSME3 and subsequently uncovered its mechanism in colon cancer.
Materials and methods: The expression of PSME3 was analyzed by using online databases, Oncomine and UALCAN. qPCR was carried out to detect the expression of PSME3 in collected colon cancer tissues and cell lines. Moreover, the promoter methylation and the hnRNA level of PSME3 were also analyzed by online database and qPCR, respectively. The candidate miRNAs targeting PSME3 were predicted by Starbase 3.0 and validated by luciferase reporter system. CCK-8, plate colon formation, and Edu incorporation were applied to study the functions of miRNA in colon cancer. The expression of miRNA and its correlation with PSME3 were detected in colon cancer tissues.
Results: Oncomine and UALCAN data indicate PSME3 is obviously upregulated in colon cancer tissue samples which is further confirmed in collected colon cancer tissues and cells by qPCR. No significant difference in methylation status promoter of PSME3 was observed between colon and colon cancer tissues. The hnRNA level of PSME3 was comparable between colon epithelial cell and colon cancer cells. miR-585-3p is predicted to directly target PSME3 and is validated by luciferase reporter assay. Then, miR-585-3p downregulation is confirmed and miR-585-3p restoration can suppress cell growth and proliferation by inhibiting PSME3 in colon cancer indicating by CCK-8, plate colon formation, and Edu incorporation. Moreover, negative correlation in expression between miR-585-3p and PSME3 was observed in our collected tissues samples.
Conclusion: We reveal for the first time that miR-585-3p downregulation accounts for the overexpression of PSME3 in colon cancer. Moreover, miR-585-3p, serving as a tumor suppressor, can inhibit cell growth and proliferation in colon cancer by targeting PSME3.

Keywords: colon, cancer, miR-585-3p, PSME3, proliferation

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