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Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip

Authors Ankireddy SR, Kim J, An SSA

Received 12 May 2015

Accepted for publication 8 July 2015

Published 25 August 2015 Volume 2015:10(Special Issue on diverse applications in Nano-Theranostics) Pages 121—128

DOI https://doi.org/10.2147/IJN.S88465

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 2

Editor who approved publication: Dr Thomas J. Webster


Seshadri Reddy Ankireddy, Jongsung Kim

Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea

Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn2+ because of the strong coordination interactions. In the presence of adenosine, Zn2+ cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.

Keywords:
quantum dots, photoluminescence, microfluidic chip, adenosine, mercaptopropionic acid, mercaptoundecanoic acid, PDMS

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