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Disinfection effects of undoped and silver-doped ceria powders of nanometer crystallite size

Authors Tsai D, Yang T, Huang Y, Peng P, Ou K

Received 6 January 2016

Accepted for publication 1 April 2016

Published 2 June 2016 Volume 2016:11 Pages 2531—2542

DOI https://doi.org/10.2147/IJN.S103760

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Lei Yang

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Dah-Shyang Tsai,1 Tzu-Sen Yang,2,3 Yu-Sheng Huang,1 Pei-Wen Peng,2,4 Keng-Liang Ou3,4

1Department of Chemical Engineering, National Taiwan University of Science and Technology, 2School of Dental Technology, 3Graduate Institute of Biomedical Materials and Tissue Engineering, 4Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei, Taiwan

Abstract: Being endowed with an ability of capturing and releasing oxygen, the ceria surface conventionally assumes the role of catalyzing redox reactions in chemistry. This catalytic effect also makes possible its cytotoxicity toward microorganisms at room temperature. To study this cytotoxicity, we synthesized the doped and undoped ceria particles of 8–9 nm in size using an inexpensive precipitation method and evaluated their disinfecting aptitudes with the turbidimetric and plate count methods. Among the samples being analyzed, the silver-doped ceria exhibits the highest sterilization ability, yet the undoped ceria is the most intriguing. The disinfection effect of undoped ceria is moderate in magnitude, demanding a physical contact between the ceria surface and bacteria cell wall, or the redox catalysis that can damage the cell wall and result in the cell killing. Evidently, this effect is short-range and depends strongly on dispersion of the nanoparticles. In contrast, the disinfection effects of silver-doped ceria reach out several millimeters since it releases silver ions to poison the surrounding microorganisms. Additionally, the aliovalent silver substitution creates more ceria defects. The synergetic combination, silver poisoning and heterogeneous redox catalysis, lifts and extends the disinfecting capability of silver-doped ceria to a superior level.

Keywords: ceria nanoparticle, antibacterial activity, cytotoxicity, oxygen vacancy, reactive oxygen species

Introduction

The cerium oxide lattice is known for its high tolerance to reversible cycles of oxygenation/deoxygenation without structure collapse enabling it to serve as an oxygen buffer in catalysis chemistry.1,2 The buffer function is made easy since cerium coexists in two oxidation states, trivalent Ce3+ and the tetravalent Ce4+. Hence, cerium oxide acts as an oxygen provider under the oxygen-deficient environment; for instance, it is added in the diesel engine at ppm level to enhance the combustion efficiency.35 After the oxide surface gives away its oxygen, Ce4+ is reduced to Ce3+ due to an extra electron left behind. And the surface reduction gives rise to oxygen vacancy formation, accompanying with color change. Researchers suggest this color variation be developed into a portable ceria-based assay for detecting food antioxidants.6 When the oxide surface is sufficiently reduced and rich in Ce3+, it is inclined to receive oxygen again. The reduced oxide may perform as an antioxidant in biomedical applications. Seal and Self have demonstrated that high-surface-area cerium oxide nanoparticles (CeONP) possess superoxide radicals scavenging capability just like superoxide dismutase.79 Other reactive oxygen species (ROS), such as hydroxyl radical,10 nitric oxide,11 and hydrogen peroxide,12 may also be scavenged by CeONP at physiological pH values. These in vitro studies suggest CeONP is a potential therapeutic agent for diseases. And pH is a decisive factor in driving CeONP to be an oxidant or antioxidant. The reports on cancer cells have also indicated a minimum toxicity toward normal mammalian tissues, and an innate cytotoxicity to cancer cells. These positive results have been reviewed and the authors point out the application opportunities of CeONP on treating the cancers associated to ROS regulation problems,13,14 but the authors also warn the readers about the inconsistency among published data.1517

Another urgent issue of the health care system is the ever-growing resistance that bacteria develop to many organic antibacterial agents because of their rapid evolution rates. CeONP provides a different mechanism on killing bacteria or restricting their cell growth. Research efforts on its toxicity could yield new approaches in disinfecting textiles, food packaging, and water. CeONP toxicity toward microorganisms is generally attributed to oxidative stress and membrane disruption. Both mechanisms require direct contact with cell wall and good dispersion of nanoparticles.1820 Nonetheless, literature reports the toxicity details differ considerably, depending on the types of microorganism, the growth media, particle-size, and pH value.2123 When biocompatible polymers, such as dextran and polyacrylic acid, are applied, more complications are involved.2427

In this work, we prepare the doped and undoped ceria particles of nanometer size using a cost-effective method and investigate their effects on growth inhibition of both the Gram-positive and -negative bacterial cells. The results show that silver-doped CeONP not only releases silver ions but also increases oxygen vacancies on the oxide surface. Thus, silver dopant introduces the disinfection effect of Ag, which has been reported effective against some drug-resistant bacteria by means of silver nanoparticles.28,29 Silver-doped CeONP further associates the two effects of ceria redox disinfection and the bacteria killing of silver synergistically and provides an alternative to upgrade the sterilization effects on bacteria and benefit the health care community.

Materials and methods

Powder synthesis and characterization

The samples of ceria nanoparticles were prepared using a facile precipitation method with caustic soda precipitant. Typically, the weighed powders of cerium nitrate were mixed with and without stoichiometric precursors of 10 mol% silver, aluminum, copper, chromium nitrates, and dissolved to make 0.1 M solutions of 1 L in volume. Precipitation occurred as 0.4 M caustic soda was added dropwise into the nitrate solution. The hydroxide precipitates of pale purple color were filtered and washed with deionized water of sufficient amount to remove residual sodium and dried at 120°C. The resulting undoped and aluminum-doped samples were yellow in color, while the silver-doped sample was black, and the chromium- and copper-doped samples appeared brown.

Phase analysis was performed with the X-ray diffractometer equipped with a copper Kα radiation source and nickel filter (Bruker D2 Phaser; Bruker AXS, Karlsruhe, Germany). The crystallite size was estimated using the Scherrer formula, 0.9λ/B cos θ, in which λ is the wavelength (0.15406 nm), and B is the full width at half maximum of the diffraction line at Bragg angle θ. Raman spectra were measured in the backscattering mode at room temperature, using a Renishaw inVia micro-Raman system equipped with a 1,800 grooves/mm grating and an optical microscope. The radiation source was an Ar ion laser beam of wavelength 514.5 nm, which was focused on a spot of 5 μm in diameter at 150 mW. Each recording was acquired after accumulation of 100 scans with an exposure time 10 seconds. The agglomerate size distribution of CeONP suspension was determined using the dynamic light scattering technique (Zetasizer, Nano ZS, Malvern Instruments, Malvern, UK). Silver concentrations of the broth, after filtering out the Ag-doped ceria sample, were measured using an inductively coupled plasma mass spectrometer (ICP-MS, JY 2000-2, HORIBA Ltd., Kyoto, Japan).

Preparation of bacterial cells and assay for antimicrobial activity

Gram-negative bacteria Escherichia coli (ATCC 25922) and Gram-positive bacteria Staphylococcus aureus (BCRC 10908), maintained as stock cultures at -80°C, were revived through growing on Luria-Bertani (LB) broth (Miller Code No 7279A) and tryptic soy broth plates (Acumedia 7164A) at 37°C for 16 hours, respectively. In this study, the pH of LB solution was adjusted to 7.3±0.2.

To evaluate growth inhibition of the ceria-based powders, growth rate and bacterial concentrations were determined by the standard turbidimetric determination and plate count methods. The plate count method begins with weighing a proper amount of ceria-based powder ranging from 0 to 80 mg in 125 mL flasks, followed by sterilization of nanoparticles in an autoclave prior to assay for the determination of antimicrobial activity. Then, 40 mL of bacterial cells suspension (5×105 colony-forming units [CFU]/mL) was added into the sterilized powder such that the final concentration fell in the range of 0–2 mg/mL in several flasks. All the flasks were subsequently incubated in the orbital shaking incubator at 200 rpm at 37°C for 24 hours. The cytotoxicity was assessed by counting CFU on LB agar plates after 37°C incubation. The cytotoxicity was also assessed by measuring the optical density (OD) at wavelength 600 nm, using a Genesys™ 10 UV-Visible spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), where OD of 0.06 at 600 nm corresponds to a concentration of 108 CFU/mL. Experiments on growth kinetics were repeated three times and data were expressed as mean ± standard deviation.

Detection of ROS

The ROS formed by silver-doped ceria nanoparticles was identified with 2′,7′-dichloro-fluorescein diacetate (DCFH-DA; Cell Biolabs, San Diego, CA, USA). Initially, 20 μL of bacterial cells suspensions, either E. coli or S. aureus, at 2×106 CFU/mL, were added into the 20 μL of 40 μM DCFH-DA at 37°C for 1 hour, followed by mixing with the prescribed amounts of silver-doped ceria nanoparticles such that the final concentrations were 0.25 and 1.0 mg/mL, respectively, for real-time probing of the intracellular ROS generation in E. coli and S. aureus cells. Finally, the ROS formed in the sample was detected using an inverted fluorescence microscope (TE2000U; Nikon, Tokyo, Japan), equipped with an objective lens (Plan Apo 60x/1.40 oil, Nikon), band-pass filters for DCFH-DA, and EMCCD camera (LucaEM DL6581; Andor, CT, USA).

Results and discussion

Doped and undoped ceria nanoparticle characterization

Figure 1A presents X-ray diffraction results of the silver-doped and undoped CeONP, which display explicit features of cubic fluorite structure. Diffraction patterns of other cation-doped ceria are not shown since they resemble that of the undoped sample. The fitted lattice parameters of doped and undoped samples, based on ten line positions of each diffraction pattern, are listed in Table 1. The lattice parameter of undoped CeONP is 0.5412 nm, consistent with the values reported in literature on ceria powder samples.30,31 Table 1 indicates the lattice parameters of Al3+-doped, Cr3+-doped samples are nearly identical to that of undoped CeONP, while that of Ag+-doped sample differs considerably, 0.5420 nm. Although the previous structure study has revealed that the lattice parameter of cerium oxide nanocrystals increases considerably when the crystallite size is <20 nm,32 the crystallite size is not the factor for lattice parameter difference because the crystallite size values (Table 1) fall in a very narrow range, 7.9 and 8.6 nm. It is more reasonable to infer that the aliovalent Ag+ doping creates more oxygen vacancies than other cation dopants, resulting in the lattice parameter difference.

Figure 1 Diffraction, Raman, and DLS analyses of ceria nanoparticles.
Notes: Contrast is made between the silver-doped and undoped CeONP samples in (A) X-ray diffraction patterns, (B) Raman spectra, and (C) DLS results. PDF 65-5923 is the standard diffraction pattern for undoped ceria of fluorite structure.
Abbreviations: CeONP, cerium oxide nanoparticles; DLS, dynamic light scattering.

Table 1 Lattice parameters of and crystallite sizes of CeONP
Notes: The lattice parameter was fitted to a cubic cell, based on the line positions of ten X-ray diffraction peaks. The crystallite size is calculated with the Scherrer formula using FWHM of the (111) diffraction line.
Abbreviations: CeONP, cerium oxide nanoparticles; CeO2, cerium oxide; FWHM, full width at half maximum.

Comparison of the Raman features of undoped and silver-doped CeONP confirms that silver doping brings forth more oxygen vacancies. The two spectra of Figure 1B show both samples are featured with a predominant peak at 457 cm−1, which results from downshifting the only allowed first-order F2g mode of the bulk crystal at 465 cm−1.33 Shifting in line position, along with noteworthy asymmetry and line broadening, is common to both doped and silver-doped CeONP. These traits have been attributed to the small crystallite size.32 The protruded feature at 540 cm−1 is unique to the sample of silver-doped CeONP, not observed in the undoped CeONP and three other doped samples. The broad band at 540 cm−1 has been identified as the contribution of oxygen vacancies,34 which strengthens the conjecture that acceptor doping of silver produces more oxygen vacancies than other dopants.

Figure 1C shows the agglomerate size distributions of undoped CeONP and Ag-doped CeONP suspensions measured with dynamic light scattering. Both measurements were taken ~10 minutes after ultrasonic treatment of two CeONP solutions of 1 mg/mL solid loading. Since no deflocculation agent is added, the average agglomerate size is 686 nm for undoped CeONP, 406 nm for Ag-doped CeONP, considerably larger than their crystallite sizes. The dispersity of two suspensions is denoted by their polydispersity index values. The polydispersity index value is 0.24 for undoped CeONP and 0.57 for Ag-doped CeONP. Both powders are rated moderately polydisperse since a monodisperse distribution requires a polydispersity index <0.2.

Antimicrobial activity of doped and undoped ceria nanoparticles

Through measuring broth turbidity, the growth curves of E. coli and S. aureus display intricate differences among the doped and undoped ceria samples, as illustrated in Figure 2A and B, respectively. The growth data were recorded in the medium inoculated with 5×105 CFU/mL of bacteria in the absence and presence of 1 mg/mL CeONP sample. Because OD of 0.06 at 600 nm corresponds to a concentration of 108 CFU/mL, the error bars are smaller than the symbol sizes when values of OD are <0.2. In addition, most of the growth curve measurements are within 10% of error; the error bars are omitted for simplicity, except for the undoped CeONP data.

Figure 2 Growth curves measured in broth turbidity expressed as OD.
Notes: In comparison with the growth curve of a blank sample without CeONP, five growth curves are plotted for those broths containing 1 mg/mL CeONP and inoculated with 5×105 CFU/mL (A) Escherichia coli and (B) Staphylococcus aureus.
Abbreviations: CeONP, cerium oxide nanoparticles; CFU, colony-forming units; h, hours; OD, optical density.

As can be seen in Figure 2, introducing the 1 mg/mL undoped CeONP caused moderate reductions in bacterial density, namely, ~37% (E. coli) and ~3% (S. aureus), as compared to the blank control group; however, introducing the 1 mg/mL silver-doped CeONP caused severe reductions in bacterial density, namely, ~85% (E. coli) and ~90% (S. aureus), as compared to the blank control group. Figure 2 also points out the unique inhibition effect of silver-doped ceria, much higher than those of the other four samples, including the undoped, Al-, Cu-, and Cr-doped nanoparticles. Growth with Ag-doped CeONP is so constrained that it cannot reach its logarithmic phase period in 24 hours. On the other hand, the rest of broths attain their steady turbidities, signifying the stationary phase growths, after ~12 hours. However, further analysis is difficult with the turbidity data, given the high degree of entanglement involved in these four curves, especially in the growth of S. aureus.

The growth curve entanglement is associated with ceria interference on the turbidity reading. Even though most of ceria particles descended to the bottom before taking measurements, there was a small amount of CeONP ultrafine particles suspended and gave rise to extra optical signal, other than the signal of bacteria. When the CeONP dose is fixed, the interference may be regarded as a common background. However, as the CeONP dose varies, the interference is difficult to estimate quantitatively. Figure 3 provides a visible example. Figure 3A presents the test-tube images of the E. coli broths dosed with increasing amount of silver-doped CeONP, from left to right; while Figure 3B shows those of the S. aureus broths. Strangely, a high content of Ag-doped CeONP does not yield a more transparent broth with a lower OD reading. Instead, the tube of 0.375 mg/mL, located in the middle (tube V of Figure 3A), is the most transparent froth for E. coli, and the tube of 1.0 mg/mL (tube IV of Figure 3B) is the most see-through broth for S. aureus. The higher turbidities at high doses are caused by suspended ceria, not bacteria. Still, the preceding turbidity measurement ensures the superiority of Ag-doped CeONP, being the most disinfecting composition, but it fails to resolve finer details of the rest less effective compositions due to ceria interference.

Figure 3 Interference of suspended ceria nanoparticles on turbidity.
Notes: The images of the Escherichia coli broths dosed with (I) 0.0, (II) 0.0625, (III) 0.125, (IV) 0.25, (V) 0.375, (VI) 0.50, (VII) 1.0, and (VIII) 2.0 mg/mL Ag-doped CeONP are shown in A while those of the Staphylococcus aureus broths dosed with (I) 0.0, (II) 0.25, (III) 0.5, (IV) 1.0, (V) 1.5, and (VI) 2.0 mg/mL Ag-doped CeONP are shown in B.
Abbreviation: CeONP, cerium oxide nanoparticles.

Since the Al-, Cu-, and Cr-doped samples display disinfection effects similar to undoped CeONP, our attention henceforth focuses on comparison of the undoped and Ag-doped samples. We turn to the standard plate count method and analyze the dose dependences of these two compositions. The plate count method examines the survival curves after 24 hours incubation, treated with ceria nanoparticles of six or seven different doses. Figure 4A indicates a rather large dosage is necessary for undoped CeONP to effectively suppress the bacterial growth, especially for E. coli. Dosing undoped CeONP, 60 mg/mL, decreases the E. coli counts by three orders of magnitude, and the S. aureus counts by six orders of magnitude in CFU/mL. Disinfection of undoped CeONP is more effective on S. aureus than E. coli.

Figure 4 Bacterial survival results of the plate count method.
Notes: Doses of (A) undoped CeONP and (B) silver-doped CeONP influence the Escherichia coli and Staphylococcus aureus survivals differently after 24 hours. Bacterial inoculation is 5×105 CFU/mL for all samples.
Abbreviations: CeONP, cerium oxide nanoparticles; CFU, colony-forming units.

In contrast, silver-doped CeONP is much more effective on both types of bacteria, compared with the undoped sample. Survival curves of Figure 4B show there is no bacteria survived when the dose is higher than 0.375 mg/mL for E. coli and 1.50 mg/mL for S. aureus. Hence, we locate the value of the minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria (MIC50), 0.25 mg/mL for E. coli, and 1.0 mg/mL for S. aureus. Inhibitory effect of silver-doped CeONP is more evident on Gram-negative E. coli than Gram-positive S. aureus, implying S. aureus possesses a potent defense system toward silver.

Visualization of killing or delaying bacterial cells of Ag-doped CeONP

Figure 5A and B present the bright-field images of E. coli (initial concentration of 5×105 CFU/mL) in the absence and presence of 2 mg/mL of silver-doped CeONP after 24 hours. Without CeONP, the microfluidic flow cell is teeming with rod-shaped bacteria (Figure 5A). While with silver-doped CeONP, the microfluidic flow cell is empty, the rod-shaped cells are killed completely. Similar contrasts can be also found in the microfluidic flow cell of S. aureus. In the absence of silver-doped CeONP, S. aureus colonies are arranged in grape-like clusters, as shown in Figure 5C. With 2 mg/mL Ag-doped CeONP, grape-like clusters are wiped out (Figure 5D). Thus, direct visualization reveals that, at a dose level higher than its MIC50, Ag-doped CeONP interrupts the growth of the bacteria cells, consistent with the results of the standard plate count method.

Figure 5 Images of bacteria with and without a large dose of Ag-doped CeONP.
Notes: Contrast is made on Escherichia coli growth (A) with and (B) without the influences of 2 mg/mL of silver-doped CeONP. A similar comparison is done on Staphylococcus aureus growth (C) with and (D) without 2 mg/mL of silver-doped CeONP.
Abbreviation: CeONP, cerium oxide nanoparticles.

To visualize more details of the Ag-doped CeONP influences under the microscope, we keep the dose level at MIC50 such that the bacterial cells are sufficiently affected but not being killed entirely. To conduct the real-time monitoring, bright-field images of bacteria were captured every 30 minutes for 120 minutes, while their intracellular ROS was quantified by the fluorescent probe DCFH-DA, and detected using an inverted fluorescence microscope equipped with an EMCCD camera. Figure 6AE shows the time-dependent changes in E. coli population of the blank control group (initial concentration of 5×105 CFU/mL, without Ag-doped CeONP). As can be seen in Figure 6, there were two individual E. coli bacterial cells at room temperature (Figure 6A) in the beginning; after 2 hours, there were eight E. coli bacterial cells (Figure 6E). Two divisions occurred during the 2-hour period. In contrast, no division occurred when E. coli population was treated with 0.25 mg/mL Ag-doped CeONP, as shown in Figure 7AE. The aforementioned contrast reveals that there is a delay in the bacterial growth of E. coli tested at the dose of MIC50 (0.25 mg/mL Ag-doped CeONP).

Figure 6 BF images of Escherichia coli growth in the blank control group.
Notes: Growth without CeONP, the incubation time increases every 30 minutes; (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. Two cell divisions occur in 2 hours.
Abbreviations: BF, bright-field; CeONP, cerium oxide nanoparticles.

Figure 7 BF images of Escherichia coli growth in presence of Ag-doped CeONP with MIC50 dose.
Notes: Growth with Ag-doped CeONP, the incubation time increases every 30 minutes with 0.25 mg/mL; (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. No division is observed.
Abbreviations: BF, bright-field; CeONP, cerium oxide nanoparticles; MIC50, minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria.

We further examined the connection between the presence of Ag-doped CeONP and the intracellular ROS formation. Figure 8 shows the time-dependent changes of ROS fluorescence signal in three individual living E. coli cells, where the corresponding bright-field images (Figure 7) were captured at the same time intervals. We then define the ROS intensity ratio, (It-I0)/I0, where I0 and It represent the ROS fluorescence signal within the individual living E. coli cell at times point 0 and t minutes, respectively, to examine the intracellular ROS kinetics induced by Ag-doped CeONP on E. coli cells (Figure 8F). The sequential fluorescence images indicate the intracellular ROS formation is significantly weak, namely, (I30I0)/I0≈0, in the first 30 minutes of incubation (Figure 8A and B). However, the fluorescence signals of ROS obtained from E. coli cells 2 and 3 light up after 60 minutes of incubation (Figure 8CE).

Figure 8 Fluorescence images of Escherichia coli growth in the presence of Ag-doped CeONP with MIC50 dose.
Notes: Growth with 0.25 mg/mL Ag-doped CeONP, the incubation time increases every 30 minutes; (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. (F) Time-dependence of the ROS fluorescence signal within the individual living E. coli cell. The ROS fluorescence signifies the intracellular ROS formation.
Abbreviations: CeONP, cerium oxide nanoparticles; MIC50, minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria; ROS, reactive oxygen species.

For comparison purpose, the same procedure is applied to analyze S. aureus population and the corresponding ROS formation. Figures 9 and 10 present the time-dependent changes of S. aureus population (initial concentration of 5×105 CFU/mL) in the absence and presence of 1.0 mg/mL Ag-doped CeONP during the first 2 hours of incubation. Figure 9E shows a pair of S. aureus that have gone through two cell divisions, producing a pair of tetrads. It demonstrates that two divisions occur in 120 minutes. However, no division occurs when S. aureus was treated with 1 mg/mL Ag-doped CeONP, as can be clearly seen from a series of images in Figure 10. Again, the earlier finding reveals that the bacterial growth of S. aureus has been obstructed in the presence of its MIC50, just like E. coli.

Figure 9 BF images of Staphylococcus aureus growth in the blank control group.
Notes: Growth without CeONP, the incubation time increases every 30 minutes; (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. Two cell divisions occur.
Abbreviations: BF, bright-field; CeONP, cerium oxide nanoparticles.

Figure 10 BF images of Staphylococcus aureus growth in the presence of Ag-doped CeONP with MIC50 dose.
Notes: Growth with 1.0 mg/mL Ag-doped CeONP, the incubation time increases every 30 minutes; (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. No cell division is seen.
Abbreviations: BF, bright-field; CeONP, cerium oxide nanoparticles; MIC50, minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria.

Similarly, the delay can be traced back to the intracellular ROS formation of S. aureus. Figure 11 presents the corresponding fluorescence images of Figure 10, showing intracellular ROS fluorescence signal in S. aureus. As can be seen in Figure 10, there are a pair of cells on the right, but only one of them glows in Figure 11A and B. When incubation time reaches 60 minutes, the other cell begins to give off fluorescence too, and this pair is visible in Figure 11CE. The higher MIC50 dose for S. aureus suggests that S. aureus possesses a potent defense system. It is rational to deduce that the comparatively higher vulnerability of E. coli resides in the difference in cell membranes. The membrane difference makes ROS formation kinetically slower; hence, Gram-positive S. aureus appears more resistant to Ag-doped CeONP.

Figure 11 Fluorescence images of Staphylococcus aureus growth in presence of Ag-doped CeONP with MIC50 dose.
Notes: Growth with 1.0 mg/mL Ag-doped CeONP, the incubation time increases every 30 minutes (A) 0, (B) 30, (C) 60, (D) 90, and (E) 120 minutes. The ROS fluorescence signifies the intracellular ROS formation.
Abbreviations: CeONP, cerium oxide nanoparticles; MIC50, minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria; ROS, reactive oxygen species.

Synergetic disinfection of silver-doped ceria

We believe the strong disinfection effect of Ag-doped CeONP is attributed to synergetic combination of two mechanisms, namely, redox catalysis of surface oxygen vacancy and silver poisoning. The argument of silver poisoning is evidenced in Figure 12A, showing the concentrations of Ag+ ion eluted from the Ag-doped CeONP in LB medium after 24 hours. When the dose of Ag-doped CeONP is 0.375 or 1.5 mg/mL, the silver ion concentration in the broth reaches 15 and 25 ppm, respectively. Furthermore, the agar diffusion method is applied to prove that the released silver is the main reason for the antibiotic rings of Figure 12B and C, where a known dose of the undoped CeONP and silver-doped CeONP is spotted on 6 mm diameter paper discs placed on the inoculated agar surface. Plates are incubated for 24 hours prior to determination of results. Figure 12B contrasts a 3 mm ring of Ag-doped CeONP and ~0 mm ring of undoped CeONP on the E. coli plate. Careful inspection indicates no E. coli growth underneath the disk of undoped CeONP, suggesting disinfection of the undoped disk is short-range and relies on physical contact. Hence, the dispersion state of undoped CeONP is critical to the sterilization effect. On the S. aureus plate, Figure 12C shows a 2 mm ring of Ag-doped CeONP and ~0 mm ring of undoped CeONP. The smaller ring of Ag-doped CeONP reflects a relatively weak effect of S. aureus, consistent with Figure 4B.

Figure 12 Silver ions eluted from Ag-doped CeONP and the antibiotic rings.
Notes: (A) Silver ion concentration of the broth is plotted against the dose of Ag-doped CeONP. Antibiotic rings are found around the disks of silver-doped CeONP in the (B) Escherichia coli and (C) Staphylococcus aureus plates. No ring is observed around the disks of undoped CeONP. These disks are dry powder compacts of nanoparticles, being pressed uniaxially.
Abbreviations: CeONP, cerium oxide nanoparticles; ICP, inductively coupled plasma; M, mega.

Figure 13 compares the disinfection difference between Ag-doped CeONP and a superposition of undoped ceria plus the released silver ions, where data points of E. coli and S. aureus survival are marked for these two experiments against the background of Figure 4B. The bacterial survival of a combination of undoped ceria plus the released silver ions is slightly higher than that of Ag-doped CeONP in E. coli and S. aureus broths. This finding not only justifies the synergetic effect but also supports the conjecture that Ag-doped CeONP produces more oxygen vacancies such that Ag-doped CeONP disinfects better than a simple combination of ceria particles and silver ions.

Figure 13 Bacteria survival in an environment of a combination of undoped CeONP and corresponding silver concentration.
Notes: Two growth experiments are designed under an environment similar to MIC50, but with a simple combination of undoped CeONP and corresponding silver ion concentration.
Abbreviations: CeONP, cerium oxide nanoparticles; CFU, colony-forming units; MIC50, minimal inhibitory concentrations required to inhibit the growth of 50% of bacteria.

Conclusion

Silver-doped CeONP has been singled out as the most disinfecting composition among the undoped and Al-, Cu-, and Cr-doped samples when the broths of E. coli and S. aureus are dosed with these nanoparticles and their sterilizations are evaluated with the turbidimetric and plate count methods. The disinfection effects of Ag-doped CeONP are also superior to a simple combination of undoped CeONP and equivalent Ag+ content. This synergetic effect goes beyond the combination of ceria redox catalysis and silver poisoning. The extra benefit may be attributed to oxygen vacancy enhancement, which is manifested in the Raman analysis on the acceptor doping of silver. The disinfection effect of Ag-doped CeONP is more pronounced on E. coli than S. aureus, although the difference is not wide.

Acknowledgments

The authors thank Taipei Medical University (TMU) and National Taiwan University of Science and Technology (NTUST) for the financial support through project TMU-NTUST-103-03. Two groups of TMU and NTUST staff contributed equally in this research work.

Author contributions

Synthesis and characterization of ceria-based nanoparticles were performed by DST and YSH. Disinfection measurements of the turbidimetric and plate count methods were carried out by TSY, PWP, KLO, and YSH. All authors contributed toward data analysis, drafting and critically revising the paper, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

Disclosure

The authors report no conflicts of interest in this work.


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