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Direct reprogramming of adult cells: avoiding the pluripotent state

Authors Kelaini S, Cochrane A, Margariti A

Received 2 November 2013

Accepted for publication 9 December 2013

Published 15 February 2014 Volume 2014:7 Pages 19—29

DOI https://doi.org/10.2147/SCCAA.S38006

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Peer reviewer comments 3


Sophia Kelaini, Amy Cochrane, Andriana Margariti

Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Institute of Clinical Sciences, Belfast, UK

Abstract: The procedure of using mature, fully differentiated cells and inducing them toward other cell types while bypassing an intermediate pluripotent state is termed direct reprogramming. Avoiding the pluripotent stage during cellular conversions can be achieved either through ectopic expression of lineage-specific factors (transdifferentiation) or a direct reprogramming process that involves partial reprogramming toward the pluripotent stage. Latest advances in the field seek to alleviate concerns that include teratoma formation or retroviral usage when it comes to delivering reprogramming factors to cells. They also seek to improve efficacy and efficiency of cellular conversion, both in vitro and in vivo. The final products of this reprogramming approach could be then directly implemented in regenerative and personalized medicine.

Keywords: ESCs, iPS, PiPS, reprogramming, transdifferentiation, miRNA

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