Dicerandrol B: a natural xanthone dimer induces apoptosis in cervical cancer HeLa cells through the endoplasmic reticulum stress and mitochondrial damage
Authors Gao DD, Guo ZM, Wang JB, Hu GF, Su YQ, Chen LJ, Lv QW, Yu HM, Qin JC, Xu W
Received 17 October 2018
Accepted for publication 3 January 2019
Published 13 February 2019 Volume 2019:12 Pages 1185—1193
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Cristina Weinberg
Peer reviewer comments 3
Editor who approved publication: Dr Sanjay Singh
Dandan Gao,1 Zhimin Guo,1 Jiabin Wang,2 Gaofeng Hu,1 Yuqiao Su,3 Lijun Chen,1 Qianwen Lv,1 Huimei Yu,2 Jianchun Qin,3 Wei Xu1
1Department of Clinical Laboratory, The First Hospital of Jilin University, Changchun 130021, China; 2Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 3Department of Biotechnology, College of Plant Sciences, Jilin University, Changchun, Jilin 130062, China
Background: Dicerandrol B is a natural antitumor agent that can be isolated from the endophytic fungus, Phomopsis sp. The present study investigated the effects of dicerandrol B on human cervical cancer HeLa cells.
Materials and methods: In this study, dicerandrol B was identified by electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. We used MTT to detect the cell viability. Flow cytometry was used to analyze the apoptosis and cell cycle. Western blot was used to examine the expression of related proteins.
Results: Dicerandrol B was isolated from the endophytic fungus Phomopsis sp. The MTT assay and flow cytometry showed that dicerandrol B significantly inhibited HeLa cell viability and induced G2/M cell cycle arrest. Western blot analysis demonstrated that dicerandrol B increased the levels of GRP78, ubiquitin, cleaved PARP, and Bax protein, decreased the levels of PARP and Bcl-2 protein, and caused an increase in the Bax/Bcl-2 ratio in HeLa cells. Dicerandrol B increased the production of ROS in HeLa cells, which was attenuated by the antioxidant N-acetyl-l-cysteine.
Conclusion: These findings suggest that dicerandrol B induces apoptosis in human HeLa cells, possibly through the endoplasmic reticulum stress and mitochondrial apoptotic pathways. This suggests that dicerandrol B possesses strong anticancer activity in cervical cancer and provides insight into the underlying mechanisms.
Keywords: apoptosis, cervical cancer, endoplasmic reticulum stress, mitochondrial damage
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