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Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe

Authors Zhang T, Tian F, Long L, Liu JB, Wu XC

Received 17 April 2018

Accepted for publication 5 June 2018

Published 23 August 2018 Volume 2018:13 Pages 4795—4805

DOI https://doi.org/10.2147/IJN.S171429

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Linlin Sun


Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4

1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, China; 4CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, China

Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools.
Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen.
Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus.
Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity.
Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions.

Keywords: gold nanorods, nanozyme, antigen conjugation, rubella virus, diagnosis

Corrigendum for this paper has been published.

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