Development of Drug Dual-Carriers Delivery System with Mitochondria-Targeted and pH/Heat Responsive Capacity for Synergistic Photothermal-Chemotherapy of Ovarian Cancer
Received 8 August 2019
Accepted for publication 28 December 2019
Published 16 January 2020 Volume 2020:15 Pages 301—313
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 4
Editor who approved publication: Dr Mian Wang
Xiaoxia Guo, 1,* Jie Mei, 1,* Chunping Zhang 2
1Department of Obstetrics and Gynecology, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Chengdu 610041, Sichuan, People’s Republic of China; 2The Center of Clinical Laboratory, Sichuan Great Master Diagnostics Co. Ltd, Chengdu 611731, Sichuan, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Chunping Zhang
The Center of Clinical Laboratory, Sichuan Great Master Diagnostics Co. Ltd, No. 8, Anhe the Second Road, Hi-Tech Industrial Development Zone, Chengdu 611731, Sichuan, People’s Republic of China
Tel +86-189 8008 6040
Email [email protected]
Purpose: Multifunctional drug delivery systems (DDS) are emerging as a new strategy to highly treat malignant tumors. The aim of this study is to develop a drug dual-carriers delivery system (DDDS) using the natural protein ferritin (FRT) and a nanoscale graphene oxide (NGO) as dual-carriers.
Methods: The FRT is a pH-sensitive hollow cage protein with disassembly and reassembly properties and the NGO has a large surface area and a photothermal effect by which it can load and release drugs under near-infrared irradiation (NIR). Due to these unique features, the NGO loaded the anticancer drug resveratrol (RSV) and the conjugated mitochondrion targeted molecule IR780 as IR780-NGO-RSV (INR), the first drug delivery platform. Next, the INR was capsulated by FRT to form the DDDS [email protected] which was applied for synergistic photothermal-chemotherapy of ovarian cancer.
Results: Through a series of characterizations, [email protected] showed a uniform nanosphere structure and remarkable stability in physiological condition. Heat/pH 5.0 was confirmed to trigger RSV release from the [email protected] After taken up by cells, [email protected] located to the lysosomes where the acidic environment triggered INR release. INR targeted the mitochondrion and released RSV to directly react with organelles, which in turn decreased the mitochondrion membrane potential and caused cell apoptosis. In-vivo experiments showed that [email protected] combined with NIR irradiation displayed remarkable tumor suppression with a high survival rate after 60 days of treatment. Finally, the biocompatibility of [email protected] was demonstrated in vitro and in vivo.
Conclusion: These results highlight the immense potential of [email protected] as a type of DDDS for the treatment of tumors.
Keywords: resveratrol, apoptosis, drug dual-carriers delivery system, mitochondrion targeting, pH/heat-triggered tumor therapy
Ovarian cancer poses a huge threat to women’s health. Although significant progress has been made to cure it, huge challenges still remain. Common methods for the treatment of ovarian cancer mainly include surgery and systemic chemotherapy.1–3 However, the treatment effect is still not ideal and recurrence is high. Additionally, currently used chemotherapy drugs have some disadvantages such as poor water solubility, rapid elimination of small molecules, lack of specific targeting, and systemic toxicity caused by large doses.4–7 Therefore, new chemical and pharmaceutical preparations, such as multifunctional drug delivery systems (DDS), are expected to significantly improve the effects of chemotherapy.8–14
In the past few decades, there have been a large number of reports on the use of nanoparticle-based DDS for cancer treatment.15–17 Compared to free small molecule chemotherapy drugs, DDS shows high bioavailability, low systemic side effects, and rich surface functional modification sites.9,17 To date, DDS based on a variety of materials such as silica nanoparticles, carbon-based materials, liposomes, proteins, nanogels, etc., have been developed.18–23 Most of them can be controllably stimulated to release drug, which prevents the off-target release and improves the therapy efficiency.24–26 However, several shortcomings in DDS limit their applications: 1) Drug leakage may be easily stimulated during delivery of the drug from the bloodstream to tumor organelles; 2) Drugs released in the cytoplasm have no target organelles, which may influence the therapy effect. Therefore, to overcome these problems, new strategies are proposed.
In this study, we developed a drug dual-carriers delivery system (DDDS) based on common-used drug carriers, ferritin (FRT) and nanoscale graphene oxide (NGO). Ferritin is a natural protein and has 24 subunits which assemble into a hollow cage-like nanostructure and disassembles in acid and neutral conditions.27–30 NGO is an excellent drug carrier as it has a large surface area, good drug loading ratio, and near-infrared (NIR) photothermal-triggered drug release.31–37 In this DDDS, NGO, the first carrier of the highly loaded resveratrol (RSV), linked with mitochondrion targeted molecule IR780 to form INR. Next, ferritin, the second carrier capsulated the INR via the disassembly of FRT under acidic conditions. The drug delivery process consisted of the following steps: 1) DDDS uptake by cells and was carried by lysosomes (acidic conditions); this denatured FRT to release INR into the cytoplasm; 2) INR targeted the mitochondrion and NIR-released RSV to directly react with the target organelle. In this design, the drug is released only in the presence of two stimuli (acid and heat) and avoids drug leakage during delivery in the circulatory system. Additionally, the first carrier can be endowed with target organelle features, which can enhance the therapeutic effect. In-vitro and in-vivo experiments demonstrated that the prepared DDDS had excellent ovarian cancer therapy effect with excellent biocompatibility.
Materials and Methods
Graphene oxide powder was purchased from Nanjing XF NANO Materials Tech Co., Ltd (China). Mitotracker red, Lysotracker red, Calcein AM/PI kit, Annexin V-FITC/PI apoptosis staining kit were purchased from Solarbio (Beijing, China). IR-780 iodide (IR780), ferritin (FRT) from equine spleen, resveratrol (RSV, ≥99%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimidehydrochloride (EDC), N-hydroxysuccinimide (NHS) and 6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (USA). All other chemicals used in the study were from Aladdin (Shanghai, China).
Cell Culture and Animal Models
The human ovarian cancer cell line SKOV-3 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured in DMEM complete media containing 10% fetal bovine serum, 1% penicillin-streptomycin, and in a 37°C constant temperature humidified carbon dioxide incubator. 5–8 weeks female Balb/c nude mice were purchased from Charles River Laboratories (Beijing, China). A SKOV-3 tumor xenograft model was constructed by subcutaneous injection of cell suspension (107 cells). All animal experiments were strictly in accordance with the Laboratory Animal Care and Protection Guidelines of Sichuan Academy of Medical Sciences and approved by the Ethics Committee of the Sichuan Academy of Medical Sciences.
Preparation and Characterization of [email protected]
Firstly, GO powder (10 mg) was added to distilled water (5 mL) and sonicated for 12 h under an ice bath. Thereafter, the mixture was centrifuged at 12,000 rpm to collect nanoscale GO (NGO) in the supernatant. IR780 was mixed with NGO (w/w=1:1), and then EDC (8 mM) and NHS (10 mM) were sequentially added, and stirred at room temperature for 3 hrs. Then, the resulting mixture was dialyzed against distilled water for 24 hrs to remove excess chemicals to obtain an IR780-linked NGO (IR780-NGO, IN). For drug loading, 4 mg/mL of RSV solution (dissolved in DMSO) was added to the IR780-NGO solution (2 mg/mL) at 4°C and stirred for 6 h. The mixture was then dialyzed against distilled water (MW cutoff = 10 kDa) to remove unbound RSV and DMSO solvent to give purified RSV-loaded IR780-NGO (IR780-NGO-RSV, INR). The concentration of bound RSV was measured by UV-vis spectroscopy (Perkin-Elmer, USA) at 306 nm.
Next, 1 mg/mL of FRT was dissolved in a buffer solution at pH 5.0 and gently stirred at room temperature for 30 min to ensure complete disassembly of the FRT. The INR was then added to the FRT solution, stirred for 10 min, and then the pH of the solution was adjusted to neutral with stirring. The resulting solution was then dialyzed against saline overnight to remove free drug and chemicals to give an INR-loaded FRT ([email protected]). The morphology and size of the prepared nanoparticles were determined by atomic force microscopy (AFM, Agilent Technologies, Inc.) and Zetasizer (NanoZS; Malvern Instruments, Malvern, UK), respectively.
pH/Heat Triggered Drug Release Study
Place the [email protected] solution in a dialysis tube (MW cutoff = 10 kDa) in a different pH release medium (25 mL PBS) with or without NIR irradiation (808 nm, 0.3 W/cm2, 3 min) and stir (100RPM). At different time points, 1 mL of solution was removed from the released media and replaced with the same volume of fresh media. The amount of RSV in the removed solution was measured using a UV-vis spectrophotometer. The drug loading rate (DLR, %) was estimated by the following formula: DLR = (Wd/Wc) x 100%, where Wd is the weight of the drug determined by a UV-vis spectrophotometer and Wc is the weight of the carrier.
Cell Uptake and Intracellular Location
The fluorescent dye fluorescein isothiocyanate (FITC) was used to label [email protected] and [email protected] In brief, 1 mg FITC was dissolved in 0.5 mL DMSO and then mixed with the nanoparticles with slight stir for 30 min. Afterwards, the mixture was dialyzed at deionized water for 24 h to remove the free FITC, resulting in FITC-labeled nanoparticles. The cells were seeded at a density of 1 × 105 cells/mL onto a confocal culture dish and incubated at 37°C for 12 hrs. FITC-labeled [email protected] and [email protected] were added to the cell culture medium. After 4 h of incubation, the cells were washed three times with PBS,38 fixed with 4% paraformaldehyde for 15 min, and then stained with DAPI, Lysotracker Red, Mitotracker Red, respectively. Fluorescence signals in the cells were observed using a confocal laser scanning microscope (Nikon A1R MP, Japan). In addition, flow cytometry (FCM) was further used to assess the fluorescent signal of dyes (including FITC, Mitotracker Red, Lysotracker Red) in cells. The treated cells were also observed by bio-TEM.
In vitro Cancer Photothermal – Chemotherapy
SKOV-3 cells were seeded in 96-well plates (2 x 104 cells/well) and incubated for 24 hrs. Different concentrations of RSV, [email protected], [email protected], [email protected] were added directly to fresh cell culture medium and incubated for 4 h in an incubator. Then, the plate was taken out, the old medium was gently removed, and fresh medium was added, and the cells were irradiated with an 808 nm laser (0.3 W/cm2, 3 min). Put it in the incubator again and continue to culture for 24 h or 48 h. At the end, cell viability was measured using the CCK-8 assay. In addition, thermal images of the treated cells and corresponding temperature changes were recorded.
In addition, the photothermal therapy effect of [email protected] on SKOV-3 cells was further verified by calcein AM and propidium iodide (PI) co-staining. As with the above cell treatment method, SKOV-3 cells were subjected to NIR irradiation and continued to culture for 48 h after RSV, [email protected], [email protected], [email protected] treatment. Then, it was stained with a mixed solution of calcein AM and PI at room temperature for 15 mins, and then washed three times with PBS. The stained cells were examined under a laser scanning confocal microscope. The excitation wavelength of calcein AM was set to 490 nm, and the excitation wavelength of PI was set to 535 nm.
Apoptosis and Mitochondrial Membrane Potential (MMP) Detection
Cells were seeded in 6-well plates (1 x 104 cells/well) for 24 h, and then treated with RSV, [email protected], INR @FRT, [email protected] + NIR, [email protected] + NIR (with the same RSV concentration 40 μg/mL) for 48 h. The cells were then collected, washed, and double stained with the Annexin V-FITC/PI kit, and then statistically analyzed for FITC and PI fluorescence in the cells using FCM. The cells treated in the same manner as above were also stained with a potential sensitive dye (rhodamine 123), and then the changes in cellular MMP were evaluated by FCM analysis.
In vivo Blood Circulation and Biodistribution Study
The normal mice were intravenous injected with RSV and [email protected] And then, blood samples were collected at different times from the orbital plexus. Each blood sample was dissolved in 900 μL of lysis buffer. The concentration of RSV and [email protected] in the blood was determined by RSV absorbance spectra of each solubilized blood sample by an UV−Vis spectrometer. The sample concentrations are defined as the percentage of injected dose per gram of tissue (ID%/g). Biodistribution in tumor and major organs was performed in tumor-bearing mice. The tumor tissues and major organs (including heart, liver, spleen, lungs and kidney) were weighed and digested by aqua regia solution overnight at 24 hrs post-intravenous injection of [email protected] and [email protected], respectively. The concentration of [email protected] and [email protected] in the collected tissue was determined by RSV absorbance spectra of each solubilized tissue by an UV−Vis spectrometer. The sample concentrations are defined as the percentage of injected dose per gram of tissue (ID%/g).
In vivo Cancer Photothermal-Chemotherapy
Nude mice bearing SKOV-3 subcutaneous xenografts were randomized into 7 groups (n = 5), and then injected with PBS, RSV, [email protected], [email protected], [email protected], [email protected] + NIR and [email protected] + NIR (containing the same RSV concentration with 5 mg/kg) via the tail vein. The samples were injected two times at day 1 and day 3. At 12 and 24 hrs after the intravenous administration of the samples, the tumor area was irradiated with a 3 min NIR laser (808 nm, 0.3 W/cm2), respectively. Mice were imaged using a NIR thermography system (FLUKE) during laser irradiation. The tumor size and weight of the mice were tested every 3 days during treatment. Tumor volume = (tumor length) x (tumor width)2/2. The relative tumor volume is equal to the tumor volume at a given time point divided by the tumor volume prior to the initial treatment. At the end, the tumor and major organs of the heart, liver, spleen, lungs and kidneys of these mice were collected, fixed in 4% formalin, embedded in paraffin, stained with H&E, and examined under a digital microscope.
All statistics are expressed as mean ± SD. Statistical significance was tested by two-tailed Student’s t test. P<0.05 or P<0.01 was considered statistically significant.
Results and Discussion
Synthesis and Characterization of [email protected]
To synthesize a DDDS, [email protected], firstly, biocompatible NGO as the first carrier was prepared through ultrasonication. Then, the NGO was conjugated with IR780, a mitochondrion specific-targeted molecule, and loaded RSV, an anticancer drug, to form a nanocomposite (INR). FRT was the second carrier to capsulate the INR via the pH-responsive disassembly and reassembly of FRT. The DDDS was used for synergistic photothermal-chemotherapy of ovarian cancer in-vitro and in-vivo (Figure 1). The morphology of the nanoparticles was characterized by AFM, which showed that the NGO and the INR were sheet-like and the [email protected] was a sphere-like structure (Figure 2A–F); their heights were ~1 nm, ~3 nm, and ~16 nm, respectively (Figure 2B, D, F). Additionally, the DLS analysis showed that the sizes of NGO, INR, and [email protected] were ~10 nm, ~13 nm, and ~26 nm, respectively (Figure 2G). These results indicate that after conjugation of IR780 and RSV loading followed by FRT capsulation, the size increased sequentially. Moreover, the UV-VIS spectrum of [email protected] showed the absorbance peak of RSV at 306 nm and IR780 at 780 nm (Figure 2H); this demonstrated the successful loading of RSV and IR780 on to the DDDS. After 7 days, it was found the [email protected] showed no obvious size change in water, FBS, saline, and cell media (Figure 2I), suggesting that the [email protected] was stable in the physiological environment. The RSV loading ratio was ~132% (W/W).
Figure 1 Schematic representation of the synthesis of drug dual-delivery system [email protected] for synergistic photothermal-chemotherapy of ovarian cancer. Abbreviations: GO, graphene oxide; NGO, nanosized graphene oxide; IR780, IR-780 iodide; NIR, near-infrared; RSV, resveratrol; FRT, ferritin.
Figure 2 (A and B) The AFM image and height profile of NGO. (C and D) The AFM image and height profile of INR. (E and F) The AFM image and height profile of [email protected] (G) The size distribution of NGO, INR and [email protected] (H) The absorption spectra of RSV, NGO, IR780, and [email protected], respectively. (I) The hydrodynamic particle size change of [email protected] in various media including water, FBS, cell media, and saline over 7 days. Abbreviations: NGO, nanosized graphene oxide; IR780, IR-780 iodide; RSV, resveratrol; FRT, ferritin; AFM, atom force microscopy; FBS, fetal bovine serum.
The Photothermal Effect of [email protected]
As shown in Figure 3A, the temperature profile of [email protected] had a quick-rising phase and reached a plateau of 61.5°C within 3 min of irradiation (808 nm, 0.3 W/cm2). As a control, the maximum temperature of FRT and [email protected] reached 26.5°C and 42.3°C, respectively, under the same conditions. These results indicated that the [email protected] has an excellent photothermal effect likely due to the conjugation of IR780 and NGO and that IR780 provided the most contribution. Additionally, the [email protected] and [email protected] retained their initial photothermal effect even after 5 cycles of NIR irradiations (Figure 3B), suggesting that both of them have great photostability. Furthermore, when the [email protected] was incubated in different temperatures, from 25°C to 85°C, it showed a stable size at <65°C and increasing size at >80°C (Figure 3C), likely due to the great resistance of FRT to denaturants including high temperatures.30,39
Figure 3 (A) Photothermal heating curves of FRT, [email protected] and [email protected] solution under 3 min 808 nm laser irradiation (0.3 W/cm2). (B) Temperature variations of [email protected] and [email protected] after the continuous irradiations of 3 min 808 nm laser for 5 cycles. (C) The size change of [email protected] from 25°C to 85°C. **P<0.01, vs other groups. Abbreviations: NGO, nanosized graphene oxide; IR780, IR-780 iodide; RSV, resveratrol; FRT, ferritin.
pH/Heat Triggered Drug Release Study
As reported in previous studies,20,30 FRT and NGO, as drug carriers, were pH and heat sensitive, respectively, and these two factors were applied for this study on drug triggered release of [email protected] As shown in Figure 4A, over 24 hrs, from a pH of 5.0 to 9.0, [email protected] seldom showed RSV release. When combining with NIR irradiation, [email protected] at a pH of 5.0 showed about 60% accumulated RSV release after 24 h, and interestingly, in pH 7.4, it still displayed no significant RSV release (Figure 4B). These results indicated that acidity and NIR irradiation-induced heat can highly trigger RSV release from [email protected] The pH values in cell lysosomes and the normal physiological environment are 5.0 and 7.4, respectively.20 Additionally, FRT is reported to disassemble into a hollow porous nanosphere under acidic conditions and reassemble into a sealed hollow nanosphere under neutral conditions.28 Thus, the stepwise release process is 1) FRT denatured to release the INR in acidic conditions 2) NGO released RSV under NIR irradiation. Either pH 5.0 or NIR irradiation alone could not trigger RSV release from the DDDS. This strategy can help avoid drug leakage caused by a single factor. Additionally, IR780 cannot be released from [email protected] at a pH of 7.4 and 5.0 combined with NIR irradiation, as shown in Figure 4C, indicating the stability of the IR780 conjugation in the DDDS.
Figure 4 (A) Release kinetics of RSV from [email protected] in pH 9.0, 7.4, 6.5 and 5.0. (B) Release kinetics of RSV from [email protected] in PBS buffer (pH = 7.4 and 5.0) with 3 min NIR irradiation (808 nm, 0.3 W/cm2). (C) Release kinetics of IR780 from [email protected] in pH 7.4 and 5.0 combined with 3 min NIR irradiation (808 nm, 0.3 W/cm2). The arrows represent the NIR irradation. Abbreviations: IR780, IR-780 iodide; RSV, resveratrol.
Cell Uptake and Intracellular Distribution of [email protected]
The cell uptake and intracellular distribution of [email protected] were analyzed by confocal microscopy and flow cytometry. As shown in Figure 5A–C, the FITC-labeled [email protected] and [email protected] incubated with cells for 4 h showed no significant difference of fluorescence intensity in the cytoplasm (Figure 5A and B), as well as cell uptake according to the flow cytometry (Figure 5C), indicating that both could enter cells via endocytosis without specificity. After entering cells, [email protected] and [email protected] were both carried into lysosomes, as shown in Figure 5A. However, the [email protected] displayed a greater intensity of yellow fluorescence (FITC green and Mitotracker red fused color) when compared to [email protected] (Figure 5B). The statistical results in Figure 5D additionally confirmed the organelle distribution of [email protected] Moreover, the distribution of [email protected] within the organelles was further confirmed using Bio-TEM, which showed retained nanoparticles in the mitochondria and lysosome (Figure 5E and F). These results confirm that the [email protected] has a high cell uptake ratio and good mitochondrial targeting. Most importantly, it demonstrated that after entering cells, [email protected] as a DDDS could first locate at the lysosomes; under acidic conditions, INR was released to the cytoplasm and then targeted the mitochondrion. The DDDS is more efficient for drug precise delivery. This specific mechanism can be presumed to be mediated by active transport and lipophilic cations mediated by organic-anion transport polypeptides, which in turn promotes targeting of nanoparticles to the mitochondria.40–42
Figure 5 (A and B) The fluorescence images of SKOV-3 cells incubated with FITC-labeled [email protected] and [email protected] for 4 hrs. Scale bar=60 μm. (C) Flow cytometry measurement of cellular FITC fluorescence intensities in SKOV-3 cells after incubation with free FITC and FITC-labeled [email protected] and [email protected] *P<0.05, **P<0.01. (D) The corresponding co-localization coefficient of FITC-labeled FITC-labeled [email protected] and [email protected] with lysosome and mitochondria in cells in A and B. **P<0.01. (E and F) The bio-TEM image of [email protected] treated cells. Abbreviations: NGO, nanosized graphene oxide; FRT, ferritin; IR780, IR-780 iodide; RSV, resveratrol; FITC, fluorescein isothiocyanate; DAPI, 4ʹ,6-diamidino-2-phenylindole; MI, mitochondria; Ly, lysosome.
In vitro Synergistic Cancer Photothermal-Chemotherapy
Figure 6A and B show the temperature of adherent SKOV-3 cells incubated with PBS, [email protected], and [email protected] under an 808 nm irradiation (0.3 W cm−2) for 3 min. The results showed that the cells treated with [email protected] had the highest temperature increase (ΔT = 30°C) compared to that treated with PBS and [email protected] cells. For the biocompatibility of the DDDS, [email protected] with a high concentration up to 0.5 mg/mL showed no significant cell viability at 24 h and 48 h (Figure 6C). The viabilities of the cells treated with various concentrations of RSV, [email protected], and [email protected] for 24 h and 48 h with or without NIR irradiation were concentration-dependent and the viability decreased with increasing concentration. The highest cell viability decrease was observed in [email protected] + NIR and 48 h-treated cells, which was about 97.2 ± 2.1% (Figure 6D–F). The results demonstrated that the combination of [email protected] and NIR had an excellent anti-cancer effect. The mechanism of the tumor therapy can be concluded with two steps: 1) [email protected] entered the cells, located in the lysosomes, and triggered the release of INR by the acidic environment of the lysosomes; 2) INR targeted the mitochondrion and was triggered to release RSV by NIR-induced heat; 3) The released RSV and hyperthermia synergistically killed the cancer cells.
Figure 6 (A) Thermal images of PBS, [email protected], and [email protected] treated cells after 3 min NIR irradiation, and (B) the corresponding temperature change curves. (C) In vitro cytotoxicity against SKOV-3 cells treated with different concentrations of [email protected] for 24 h and 48 h. (D) In vitro cytotoxicity against SKOV-3 cells treated with different concentrations of RSV for 24 h and 48 h. In vitro cytotoxicity against SKOV-3 cells treated with different concentrations of [email protected] and [email protected] with or without NIR irradiation for (E) 24 h and (F) 48 h. **P<0.01, vs the other groups at the same concentration. Abbreviations: NGO, nanosized graphene oxide; FRT, ferritin; IR780, IR-780 iodide; RSV, resveratrol; NIR, near-infrared.
Cell Apoptosis and MMP Detection
As shown in Figure 7A, the control and [email protected] cells displayed green fluorescence, suggesting no cytotoxic properties. In the [email protected] and [email protected] groups, most of the dead cells showed red fluorescence, likely due to the chemotherapy and photothermal therapy. However, [email protected] combined with NIR irradiation killed almost the all cells, showing an excellent synergistic cancer photothermal-chemotherapy.
Figure 7 (A) The calcium AM/PI dual-staining images of cells after treatment by control (PBS), free RSV, [email protected], [email protected], [email protected] + NIR, and [email protected] + NIR (with the same RSV concentration 40 μg/mL) for 48 h, respectively. (B) Cell apoptosis and (C) corresponding apoptosis rate of cells treated with PBS (control), free RSV, [email protected], [email protected], [email protected] + NIR, and [email protected] + NIR (with the same RSV concentration 40 μg/mL) for 48 h by flow cytometry, respectively. **P<0.01, vs the other groups. (D) The change of MMP of SKOV-3 cells treated with PBS (control), free RSV, [email protected], [email protected], [email protected] + NIR, and [email protected] + NIR (with the same RSV concentration 40 μg/mL) for 48 h by flow cytometry, respectively. The NIR irradiation uses 808 nm laser at 0.3 W/cm2 for 3 min. **P<0.01, vs the other groups. Abbreviations: NGO, nanosized graphene oxide; FRT, ferritin; IR780, IR-780 iodide; RSV, resveratrol; NIR, near infrared; MMP, mitochondrial membrane potential.
An annexin V-FITC/PI double staining kit was utilized to detect the type of cell death induced by [email protected] As shown in Figure 7B and C, the cells treated with control and [email protected] showed hardly any dead cells. In the [email protected], [email protected] + NIR, and [email protected] + NIR-treated groups, the apoptosis rate was 61.3 ± 2.6%, 72.7 ± 3.1% and 96.8 ± 3.3%, respectively (Figure 7C). These findings suggest that the [email protected] + NIR killed cancer cells mediated by apoptosis, likely because of the released RSV in the cytoplasm.43–45
Mitochondrion play an important role in cell apoptosis. The decrease of MMP has been reported as the key event in the mitochondrial (intrinsic) apoptotic pathway. As shown in Figure 7D, in the [email protected], [email protected] + NIR, and [email protected] + NIR-treated groups, the MMP showed a 59.4 ± 3.5%, 68.3 ± 3.2% and 89.8 ± 4.1% decrease, respectively. From these results, it can be concluded that the [email protected] + NIR induced cell death that was mediated via the mitochondrial (intrinsic) apoptotic pathway.46–49
In vivo Blood Circulation and Biodistribution
Figure 8A shows the blood circulation time of free RSV and [email protected] after intravenous injecting into mice. [email protected] displayed the half-life time of 7.13 ±0.3 h. While free RSV was quickly removed from the blood-circulating system (t1/2=1.36 ±0.65 h). The blood circulation time of free RSV was highly prolonged, mainly due to the PEG and FRT encapsulation. Furthermore, 24 h post-injection with the nanoparticles, the content of RSV in the tumor and major organs tissue was investigated. As shown in Figure 8B, in the major organs, the nanoparticles mainly accumulated in liver, indicating that these nanoparticles were mainly metabolized through a hepatic pathway. In addition, higher content of [email protected] was detected in the tumor tissue compared with that of [email protected], because of the enhanced permeability and retention (EPR) and the IR780 targeting effect to mitochondrion.
Figure 8 (A) Blood circulation curves of free RSV and [email protected] in mice after intravenous injection determined by the RSV absorbance from diluted tissue lysate (The accumulation of RSV was calculated to be ID% per gram (the percentage of the injected dose per gram of tissue). (B) Content of [email protected] and [email protected] in major organs and tumor at 24 h post-treatment with nanoparticles determined by the RSV absorbance from diluted tissue lysate. **P<0.01.
In vivo Synergistic Cancer Photothermal-Chemotherapy
Encouraged by the excellent in-vitro synergistic cancer therapy, an in-vivo tumor therapy was investigated in an animal model. SKOV-3 xenografted tumors were generated in nude mice and divided into 7 random groups which were treated with PBS, RSV, [email protected], [email protected], [email protected], [email protected] +NIR, and [email protected] + NIR, respectively. The NIR irradiation was conducted at 12 h and 24 h post-injection. During the 3 mins of NIR irradiation, the highest temperature increase of 25°C was observed in the [email protected] -treated tumor, which was higher than that of the PBS (control) and the [email protected] treated tumors (Figure 9A and B). After treatment for 1 month, the body weight of all the groups showed no significant decrease (Figure 9C). For their relative tumor volume, compared to other groups including PBS, RSV, [email protected], [email protected], [email protected], [email protected] +NIR, [email protected] +NIR exhibited excellent tumor suppression without relapse (Figure 9D). Furthermore, after 2 months of initial treatment, the survival rate of mice in the [email protected] +NIR-treated group had a 100% survival rate, which was higher than that of the other groups (Figure 9E). Figure 9F shows the HE staining images of tumor tissue. A large area of apoptosis was observed in tumors treated with [email protected] + NIR compared to other groups. Moreover, major organs, including the lung, heart, liver, spleen, and kidney in these treated groups, were harvested for histological analysis. As shown in Figure 9G, the H&E stained sections of [email protected] +NIR showed no noticeable organ damage when compared with those of the control group. These results indicated that [email protected] combined with NIR irradiation had splendid biocompatibility features and excellent in-vivo anticancer efficacy mostly due to the precise targeting of organelles and high-efficient pH/heat triggered drug release.50–55 The results demonstrated that the [email protected] has no significant cytotoxicity in vivo, which indicates its excellent biocompatibility.
Figure 9 (A) The thermal images and (B) corresponding temperature statistical results of tumor-bearing mice post-tail vein injection of control PBS, [email protected] and [email protected] at 24 h under 3 min NIR irradiation (808 nm, 0.3 W/cm2), respectively. (C) Body weight of tumor-bearing mice after various treatments. (D) The growth profile of SKOV-3 xenografted tumors after intravenous injection of PBS, RSV, [email protected], [email protected], [email protected], [email protected] + NIR and [email protected] + NIR (808 nm, 0.3 W/cm2). **P<0.01, vs the other groups. (E) The survival rate of tumor-bearing mice after various treatments for 60 days. (F) Micrographs of H&E-stained tumor slices collected from PBS-, RSV-, [email protected], [email protected], [email protected], [email protected] + NIR- and [email protected] + NIR- (808 nm, 0.3 W/cm2) treated groups at the end of the treatment. Scale bar = 50 μm. (G) H&E-stained tissue sections of major organs, including the heart, liver, spleen, lung, and kidney from tumor-bearing mice in saline (control) and [email protected] + NIR groups after the treatment. Scale bar = 100 μm. Abbreviations: NGO, nanosized graphene oxide; FRT, ferritin; IR780, IR-780 iodide; RSV, resveratrol; NIR, near-infrared.
In summary, we have designed and prepared a multifunctional [email protected] DDDS based on the FRT protein and the NGO. In this system, the NGO sheets, as the first drug carrier, highly loaded RSV and conjugated mitochondrion IR780 to form INR. FRT, as the second carrier, capsulated the INR via the disassembly of FRT under acidic conditions. The resulted [email protected] showed excellent physiological stability and biocompatibility. Due to the contribution of IR780 and NGO to NIR absorbance, [email protected] was confirmed to be a splendid photothermal agent. Under the acidic conditions, FRT disassembled to release the INR. NIR irradiation triggers RSV release from NGO; and after entering cells, [email protected] enters the lysosomes, providing an acidic environment to trigger FRT release of INR. With the guidance of IR780, INR targeted the mitochondrion and released RSV under the NIR irradiation. The multifunctional [email protected] accompanied with pH/heat-triggered RSV release showed a great synergistic suppression of tumor growth both in-vitro and in-vivo without systemic toxicity. These results demonstrate that the [email protected] can be a potential and high-efficiency DDDS for cancer therapy.
The authors report no conflicts of interest in this work.
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