Back to Journals » International Journal of Nanomedicine » Volume 6

Detection of micrometastases in peripheral blood of non-small cell lung cancer with a refined immunomagnetic nanoparticle enrichment assay

Authors Li Q, Qi H, Zhou H, Deng C, Zhu H, Li J, Wang X, Li Q

Published 29 September 2011 Volume 2011:6 Pages 2175—2181


Review by Single-blind

Peer reviewer comments 3

Qing Li1, Hui Qi1, Han-Xin Zhou1, Chun-Yan Deng1, Hai Zhu3, Jin-Feng Li3, Xi-Li Wang3, Fu-Rong Li1,2
1Clinical Medical Research Center, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen, People's Republic of China; 2Shenzhen Institute of Gerontology, Shenzhen, People's Republic of China; 3Shenzhen Bioeasy Biotechnologies Co Ltd, Shenzhen, People's Republic of China

Abstract: Fe3O4 particles are currently used as the core of immunomagnetic microspheres in the immunomagnetic enrichment assay of circulating tumor cells (CTCs). It is difficult to further improve the sensitivity of CTC detection or to improve tumor cell-type identification and characterization. In the present study, we prepared immunomagnetic nanoparticles with nanopure iron as the core, coated with anti-cytokeratin 7/8 (CK7/8) monoclonal antibody. These immunomagnetic nanoparticles (IMPs) were used in conjunction with immunocytochemistry (ICC) to establish a refined immunomagnetic nanoparticle enrichment assay for CTC detection in non-small cell lung cancer (NSCLC). The assay was compared with nested reverse transcription polymerase chain reaction (RT-PCR) to detect CK19 mRNA and lung specific X protein (LUNX) mRNA. Human lung adenocarcinoma cell line A549 was used for sensitivity and specificity evaluation. Peripheral blood samples were collected from each group for CTC detection. The average diameter of the immunomagnetic nanoparticles was 51 nm, and the amount of adsorbed antibodies was 111.2 µg/mg. We could detect down to one tumor cell in 5 × 107 peripheral blood mononuclear cells. The sensitivity was consistent with that of nested RT-PCR; however, the false positive rate was significantly reduced. The modified assay combined with ICC did not differ from nested RT-PCR in sensitivity, but it had significantly increased specificity. This approach could, therefore, contribute to identification of micrometastases, re-defining clinical staging, and guiding individual postoperative treatments. The technique shows considerable potential clinical value and further clinical trials are warranted.

Keywords: NSCLC, circulating tumor cells, nested RT-PCR, immunomagnetic nanoparticles, immunocytochemistry

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other article by this author:

Readers of this article also read:

Gum arabic-coated radioactive gold nanoparticles cause no short-term local or systemic toxicity in the clinically relevant canine model of prostate cancer

Axiak-Bechtel SM, Upendran A, Lattimer JC, Kelsey J, Cutler CS, Selting KA, Bryan JN, Henry CJ, Boote E, Tate DJ, Bryan ME, Katti KV, Kannan R

International Journal of Nanomedicine 2014, 9:5001-5011

Published Date: 28 October 2014

Evaluation of psoralen ethosomes for topical delivery in rats by using in vivo microdialysis

Zhang YT, Shen LN, Zhao JH, Feng NP

International Journal of Nanomedicine 2014, 9:669-678

Published Date: 23 January 2014

Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review

Islam MA, Firdous J, Choi YJ, Yun CH, Cho CS

International Journal of Nanomedicine 2012, 7:6077-6093

Published Date: 13 December 2012

Practical preparation procedures for docetaxel-loaded nanoparticles using polylactic acid-co-glycolic acid

Keum CG, Noh YW, Baek JS, Lim JH, Hwang CJ, Na YG, Shin SC, Cho CW

International Journal of Nanomedicine 2011, 6:2225-2234

Published Date: 7 October 2011

Effects of a novel pH-sensitive liposome with cleavable esterase-catalyzed and pH-responsive double smart mPEG lipid derivative on ABC phenomenon

Chen D, Liu W, Shen Y, Mu H, Zhang Y, Liang R, Wang A, Sun K, Fu F

International Journal of Nanomedicine 2011, 6:2053-2061

Published Date: 20 September 2011

Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Generalov R, Kavaliauskiene S, Westrøm S, Chen W, Kristensen S, Juzenas P

International Journal of Nanomedicine 2011, 6:1875-1888

Published Date: 7 September 2011

Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

Xu W, Ganz C, Weber U, Adam M, Holzhüter G, Wolter D, Frerich B, Vollmar B, Gerber T

International Journal of Nanomedicine 2011, 6:1543-1552

Published Date: 2 August 2011

Nanotoxicology and nanoparticle safety in biomedical designs

Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, Aminifard S, Zafari M, Akbari HR, Rad HG

International Journal of Nanomedicine 2011, 6:1117-1127

Published Date: 31 May 2011