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Detection of a new reaction by-product in BDDE cross-linked autoclaved hyaluronic acid hydrogels by LC–MS analysis

Authors Fidalgo J, Deglesne PA, Arroyo R, Sepúlveda L, Ranneva E, Deprez P

Received 2 March 2018

Accepted for publication 21 July 2018

Published 15 October 2018 Volume 2018:11 Pages 367—376


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Scott Fraser

Javier Fidalgo,* Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Lilian Sepúlveda,* Evgeniya Ranneva, Philippe Deprez

Scientific Department, Skin Tech Pharma Group, Castello D’Empúries, Cataluña, Spain

*These authors contributed equally to this work

Background: Hyaluronic acid (HA), a naturally occurring polysaccharide, is used in the production of dermal fillers for esthetic purposes. As it has a few days of half-life in human tissues, HA-based dermal filler is chemically modified to increase its lifetime in the body. The most common modification used in commercial HA-based filler is the cross-linking of HA chains using 1,4-butanediol diglycidyl ether (BDDE) as cross-linking agent. Residual, or unreacted, BDDE is considered nontoxic when it is <2 parts per million (ppm); therefore, the quantification of residual BDDE in the final dermal filler is mandatory to ensure the safety of the patients.
Materials and methods: The present study describes the detection and characterization of one by-product of the cross-linking reaction between BDDE and HA in alkaline conditions by combining both liquid chromatography and mass spectroscopy (LC–MS).
Results: After different analyses, it was found that the alkaline conditions and the high temperatures employed to sterilize the HA–BDDE hydrogel promote the formation of this new by-product, a “propene glicol-like” compound. LC–MS analysis confirmed that this by-product have the same monoisotopic mass as that of BDDE, a different retention time (tR), and also a different UV absorbance (λ=200 nm) pattern. Unlike BDDE, it was observed in the LC–MS analysis that this by-product had a higher detection at 200 nm in the same assay conditions.
Conclusion: These results suggest that this new compound does not have an epoxide on its structure. The discussion is open to assess the risk of this new by-product found in the production of HA–BDDE hydrogels (HA dermal fillers) for commercial purposes.

Keywords: hyaluronic acid, HA dermal fillers, cross-linked hyaluronic acid, BDDE, LC–MS analysis, BDDE by-product.

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