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Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum

Authors Devanesan S , Ponmurugan K , AlSalhi MS , Al-Dhabi NA

Received 28 April 2020

Accepted for publication 9 June 2020

Published 19 June 2020 Volume 2020:15 Pages 4351—4362


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Thomas J Webster

Sandhanasamy Devanesan, 1, 2 Karuppiah Ponmurugan, 3 Mohamad S AlSalhi, 1, 2 Naif Abdullah Al-Dhabi 3

1Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia; 3Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia

Correspondence: Mohamad S AlSalhi
Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia
Email [email protected]

Purpose: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluated its biological properties.
Materials and Methods: The silver nanoparticles synthesized using asafoetida gum (As-AgNPs) were characterized using UV/Vis spectroscopy, fourier infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM) and EADX. The cytotoxicity and antimicrobial activity As-AgNPs were evaluated against MCF-7 cell lines and selected microbial pathogens, respectively.
Results: The synthesized silver nanoparticles were crystalline in nature with a spherical shape. The average particle size was 5.6– 8.6 nm. The cytotoxicity of the synthesized As-AgNPs was evaluated against MCF-7 cell lines, and the As-AgNPs were found to be effective in inhibiting the multiplication of cancer cells. The As-AgNPs exhibited significant antimicrobial activity towards E. coli, K. pneumoniae and C. albicans. The MIC of the synthesized As-AgNPs was 7.80 μg/mL for E. coli ATCC 25922, Salmonella sp. WS50- and S. typhi; 15.60 μg/mL for S. typhimurium and S. aureus WS10, and 31.20 μg/mL for K. pneumoniae and S. aureus ATCC 43300-MRSA. In addition, MIC values of 15.60 μg/mL for C. albicans ATCC8436 and 31.20 μg/mL for C. krusei ATCC6258 were obtained.
Conclusion: As asafoetida is a good traditional medicine, its involvement in the synthesis of AgNPs led the silver nanoparticles to exhibit good cytotoxic and antimicrobial effects.

Keywords: asafoetida gum, AgNPs, antimicrobial, cytotoxicity, biomedical agent

Corrigendum for this paper has been published


Asafoetida is the dried latex extruded from the rhizome of a perennial herb, Ferula foetida. Traditionally, asafoetida gum is used for digestive benefits and is a kitchen consumable in many South Indian homes. It gives a pleasant flavor to foodstuffs and is a good antioxidant, with antimicrobial and anticancer properties.1 The prime constituents of asafoetida latex include resin (above 60%), endogenous gum (above 20%), volatile oil (above 15%) and ash (less than 10%). Asafoetida has been used as a sedative agent for neuropathic patients because it induces sleep without any adverse effects.2,3 Asafoetida latex extract has been reported to reduce serum cholesterol and blood pressure.4 Recently, asafoetida in combination with other organosulfur compounds was reported to stimulate neutrophil activities. It is capable of altering the biological response process by increasing phagocyte roles.5 Asafoetida gum has been reported as a reducing agent for neurological diseases, and it reduces the oxidative stress marker malondialdehyde and increases superoxide dismutase in brain tissue.6 Ethanol extract of asafoetida gum has been shown to enhance insulin secretion and to reduce fasting sugar levels in Wistar rats, and it functions as a hypoglycemic and hypolipidemic agent.7 Chinese physicians use asafoetida extract to treat heart diseases.8 Asafoetida is used for many respiratory ailments, including H1N1 swine flu. In folk medicine, it is used as a remedy for many purposes, including to provide carminative, antispasmodic, expectorant, sedative, diuretic and many other effects.

An antimicrobial study carried out using asafoetida extracts showed good inhibitory action towards both Gram-positive and Gram-negative bacterial pathogens.9 The bioactive compounds isolated from asafoetida were tested against nosocomial infections, and the result showed that the bacterial strains that were resistant to drugs responded well, confirming the antimicrobial potential of the isolated compounds.10 The toxicity of AgNPs has become a controversial because the molecular mechanism of nanosilver is focused on the surface, it can be easily oxidized by O2 and several molecules in the environmental and biological systems due to the release of Ag+. The toxicity is mostly related to the release of toxic ion (Ag+).11 The surface oxidation rate is closely related to the nanosilver surface coating of coexisting molecules like thiol derivatives and to assist the right environments. The nanosilver can be capable to interact with nucleic acids, lipid molecules, and proteins; further, it also penetrates into the cell and also incorporates within a biological system. The nanosilver can cause oxidative stress via ROS and damage the complex biomolecules system including DNA, enzymes, proteins and cell membranes.12

The cytotoxicity effects of asafoetida resin have been reported in NB4 and HL-60 cell lines, and asafoetida resin induced apoptosis in cancer cell lines without affecting normal cell lines. Furthermore, no toxic effects were recorded even at high doses.13 Another study on the cell viability of HeLa cells by using F. gummosa extracts revealed dose- and time-dependent action as a radiosensitizer agent in cervical cancer.14 The compounds isolated from asafoetida gum exhibited good cytotoxic effects in HepG2, Hep3B, and MCF-7 cancer cell lines.15

The selection of reducing agents is very important in the field of green synthesis of silver nanoparticles. The chemical reduction method is one of the ideal and widely used methods with a variety of organic and inorganic reducing agents. The medicinal plant extract was used for the synthesis of silver nanoparticles which consist of various reducing agents like phenolic, polyphenols and flavonoid compounds.16 In addition, alkaloids, proteins, amino acids, alcoholic compounds, and polysaccharides are act as reducing agents during the synthesis of nanoparticles. The reduction reactions of silver ions into silver nanoparticles were completed with reducing agents.17 The current study aimed to synthesize silver nanoparticles (AgNPs) using aqueous extracts of asafoetida gum and to evaluate their antibacterial, antifungal and cytotoxic effects. Information on the synthesis of AgNPs using asafoetida gum is scant in the literature. Hence, the present study was designed to elucidate its role in nanomedicine.

Materials and Methods

Preparation and Synthesis of Asafoetida (As)-AgNPs

Asafoetida is an oleoresin exuded from the rhizome of the plant Ferula foetida. Five hundred grams of asafoetida gum was purchased from a supermarket in the Riyadh region of Saudi Arabia. The sample was washed with tap and distilled water to remove unwanted fine particles and allowed to dry. The gum was ground into a fine powder. Twenty-five mg of the fine powder was dissolved in 500 mL of Milli Q water and filtered. The filtrate was designated as the aqueous extract of asafoetida and was used for further experiments.

To prepare silver nitrate nanoparticles, 84.94 mg of silver nitrate (1 mM) was dissolved in 500 mL of Milli Q water, to which 20 mL of freshly prepared asafoetida extract was added. The reaction mixture was monitored, and the pale yellow reaction mixture became brown in 24 h. The colored mixture was centrifuged at 18000 rpm for 15 min and then washed. The process was repeated three times. The sediment obtained was dried in a vacuum evaporator. The dried powder was designated as As-AgNPs and stored for further study.

Characterization of As-AgNPs

UV-Vis Spectroscopy

UV-Vis spectroscopy (PerkinElmer LS 40, USA) was used to evaluate the reduced state of AgNPs in an optical density range of 200 to 800 nm.

Attenuated Total Reflection (ATR)

The total reflectance of As-AgNPs was measured by ATR spectroscopy using a Perkin Elmer instrument (USA). ATR was used to measure the active constituents in the synthesized materials, and the scanning range was 4000–650 cm−1.

X-Ray Diffraction (XRD)

The XRD patterns of the synthesized particles were observed using an X-ray diffractometer operated at 40 kV with 2θ values from 20 to 50. The results were compared with the JCPDS library to confirm the crystalline nature of the AgNPs.

AgNP Size and Shape Measurements

TEM investigation was carried out by using a JEM-1230 (JEOL, Tokyo, Japan) with high resolution to measure the particle shape, size and distribution. In addition, SEM and EDS analyses were performed in our earlier studies to determine the size and element distribution.1820

Cytotoxicity Assessments and Morphological Study

An MTT assay was performed following the method of Mosmann et al,21 with certain modifications. The MCF-7 cell line obtained from ATCC was grown in DMEM medium, asafoetida gum supplemented with fetal bovine serum (FBS) (10%), sodium bicarbonate (0.2%), and antibiotic-antimycotic solution (1 mL/100 mL of medium). In brief, MCF-7 cells (10000 in number) were seeded into 96-well tissue culture plates and incubated overnight in a CO2 incubator. Then, the medium was aspirated and replaced with medium containing different concentrations (1–100 µg/mL) of synthesized nanoparticles. The cells were treated for 24 h in the nanoparticle medium. Then, MTT reagent (10 µL/well containing 100 µL of cell suspension; 5 mg/mL stock in PBS) was added to each well and incubated for 4 h at 37°C. After that, the reaction mixture was carefully removed, and 200 μL of DMSO was added to each well by pipetting up and down several times until the content was homogenized. After 10 min, the color was read at 550 nm using a UV-Vis spectrophotometer. An untreated control was also run simultaneously under identical conditions.

To study morphological changes, MCF-7 cells were exposed to different concentrations (1–100 µg/mL) of nanoparticles for 24 h. Then, the nanoparticle-induced morphological changes in the cells were observed under a phase-contrast inverted microscope. The cell images were collected at 20 x magnification.

Microbial Pathogens

The bacterial pathogens selected for the current investigation were acquired from IMTECH (MTCC), Chandigarh and ATCC (Manassas, USA). The microbial isolates were Staphylococcus aureus ATCC 43300 (MRSA), Enterococcus faecium VR 83,198 (VRE), Staphylococcus aureus WS 10, Escherichia coli ESBL 937316, Escherichia coli ATCC 25922, Salmonella typhi WS 03, Salmonella typhimurium ATCC 14028, Salmonella sp. WS 50, Acinetobacter baumanni MDR 4414 and Klebsiella pneumoniae ATCC 700603. The fungal pathogens included Candida albicans ATCC 8436, Candida krusei ATCC 6258 and Candida parapsilosis ATCC 22019. All the cultures were periodically subcultured and maintained in potato dextrose agar (PDA) medium.

Preparation of Microbial Inoculums

One loop of bacterial culture was collected from the bacterial cultures, which were grown on nutrient agar slants, and inoculated into 10 mL of nutrient broth. Then, the broth culture was incubated for 18 h at 37°C. The culture obtained was used as inoculum for the sensitivity bioassay. The final bacterial inoculum size was used standard 0.5 McFarland (1 X 108 CFU/mL) concentrations. The fungi were cultured on Sabouraud dextrose broth (SDB) slants at 30°C for 24 h and the final inoculum size was 2 X 106 PFU/mL.

Antimicrobial Activity

The antibacterial activity of the asafoetida-mediated silver nanoparticles (As-AgNPs) was tested by the agar diffusion method as described by Bonev et al.22 A total of 0.1 mL of bacterial or fungal culture was seeded on Mueller-Hinton agar (MHA) plates. Six-mm-diameter wells were made on the MHA plates with a steel-core borer, and then the wells were loaded with 15, 25 and 50 µL of the As-AgNPs. Streptomycin (10 mcg), nystatin (50 mcg), and DMSO (1 mL) were used as positive and negative controls. The bacterial and fungal culture plates were incubated at 37°C and 30°C for 18 h and 24 h, respectively. After incubation, the zone of inhibition was measured, and the values were recorded.

Minimal Inhibitory Concentration (MIC) Determination

The minimum inhibitory concentrations (MICs) of the As-AgNPs were evaluated using the microdilution method described by Bonev et al.22 Briefly, the As-AgNPs were dispersed in DMSO and sterile distilled water at a ratio of 20:80. The MIC was determined by using 96-well microtiter plates. The total mixture contained 185 µL of nutrient broth, 10 µL of As-AgNPs and 5 µL of the log-phase culture of microbial pathogens. The suspension was mixed completely and diluted two-fold before adding the bacterial pathogens. Finally, 5 µL of the microbial pathogens were loaded into each well and mixed thoroughly. Subsequently, the microtiter plates were incubated for 24 h at 37°C, and streptomycin was used as a positive control. The microtiter plates were observed for bacterial growth after incubation.

Statistical Analysis

The results were analyzed by using the software Graphpad InStat 6.0. The mean ± SD and EC50 values were determined, and the values were compared by using one-way ANOVA. Data are expressed with a significance level of p <0.05.

Results and Discussion

Biosynthesis and Characterization of As-AgNPs

The asafoetida gum aqueous extract was light yellow in color (Figure 1A and B), and upon the addition of colorless AgNO3 solution, the color of the mixture turned dark brown due to surface Plasmon resonance (SPR) within 24 h, indicating the formation of silver nanoparticles. A strong absorption peak was observed in the UV-Vis absorption spectrum at 426 nm at different time intervals (24–72 h) (Figure 1C). The peak was due to the large number of molecules that were widely dispersed in the mixture solution, confirming our previously reported results.1820 The stability of synthesized As-AgNPs was assessed with the same experimental protocol repeated. The synthesis process was monitored by visual observation by the change of color and agglomeration within a period of time (24–72 h). The synthesized As-AgNPs were characterized with UV-Vis spectroscopy to evaluate stabilization and recorded the same absorption peak at 420 nm. The observed result has confirmed the stability of synthesized As-AgNPs.

Figure 1 Asafoetida gum extracts, (A) representative before formation nanoparticles asafoetida gum extracts (B) silver nanoparticles formed after reaction of asafoetida gum extracts with 1 mM of silver nitrate (C) UV-Vis spectrum of As-AgNPs at 426 nm with different time intervals (24–72 h).

The XRD patterns of the formed nanoparticles showed four distinct diffraction peaks, and Braggs reflections were observed in the XRD patterns at 2θ=38.90, 44.26, 64.43 and 77.38. The corresponding values were 111, 200, 220 and 311, respectively, indicating the crystalline nature of the Ag NPs (Figure 2). The results were compared with the JCPDS library [JCPDS No. 04–783], and the results revealed the high purity of the Ag NPs, according to previous literature.1820

Figure 2 X-ray diffraction pattern of silver nanoparticles synthesized using As-AgNPs.

The properties of the synthesized As-AgNPs were studied by using ATR-IR spectroscopy (Figure 3). The IR spectrum bands at 2923, 2193, 1730, 1611, 1120 and 834 cm−1 further confirmed the properties of the silver nanoparticles. The IR spectral peaks confirmed that the silver ions were incorporated with asafoetida gum, as indicated by the presence of hydroxyl and carboxyl groups.23

Figure 3 The ATR-IR spectrum of synthesized As-AgNPs.

SEM analysis was performed to visualize the size and shape of the silver nanoparticles. SEM images of the As-AgNPs are given in Figure 4A. The SEM results revealed that the average size was 5.60–8.66 nm with inter-particle dispersion. The differently shaped AgNPs were used as capping agents in the asafoetida gum extract. The EDX results showed evidence of the presence of Ag in the asafoetida gum-mediated nanomaterial (Figure 4B). The data obtained showed distinct spherical poly-dispersed AgNPs with a small size and without significant agglomeration. Such small nanoparticles have an enhanced surface area, thereby promoting good reactivity and catalytic properties.24 Particle size enhances nanoparticle interactions in necrotic tissues via vascular gaps in cancerous cells.25 The enhanced permeability and retention (EPR) effect due to the small size of nanoparticles helps in the high accumulation of NPs in tumor areas.26,27 Green synthesized NPs contain several active compounds, which enhance the bioactivities of these NPs. Scavenging activities have been observed in AgNPs prepared using different natural polyphenols, such as epigallocatechin-3-gallate, resveratrol, and fisetin.28

Figure 4 (A) SEM image of synthesized As-AgNPs, (B) distribution of elements analysis from As-AgNPs by EADX.

The TEM analyses focused on the morphology and particle size distribution profile of the formed As-AgNPs, as shown in Figure 5. The As-AgNPs have a spherical shape with an average size range of 5.0–7.8 nm, which is in agreement with the SEM images. The data obtained showed distinct spherical polydispersed AgNPs with a small size and without significant agglomeration. Such small nanoparticles have an enhanced surface area, thereby promoting good reactivity and catalytic properties.24

Figure 5 TEM images of synthesized As-AgNPs with JEM-1230 and showing the shape, size and distribution of NPs.

Cytotoxicity Activity of As-AgNPs

The cytotoxicity effect of the nanoparticles synthesized using asafoetida extract on MCF-7 cells was studied using an MTT assay. The microscopic analysis confirmed the toxic effect of the synthesized AgNPs against MCF-7 cell lines (Figure 6A (1–8)). Morphological observations showed nuclear hypertrophy, cell shrinkage, nuclear condensation and fragmentation, apoptosis and cell death. The control cells showed intact nuclei and cytoplasm. The viability of the MCF-7 cell line against different doses showed that the AgNPs synthesized using asafoetida extract were able to inhibit cell viability to a great extent in a dose-dependent manner. The cytotoxicity effects of different concentrations of synthesized AgNPs under in vitro conditions on the MCF-7 cell line showed a maximum IC50 value of 2.0 µg/mL (Figure 6B) and have not used any proven anti-cancer drug as a positive control. However, the cytotoxic responses of nanoparticles were compared with the untreated control set of the cells.

Figure 6 (A) Morphological changes of MCF-7 tumor cell lines, images with phase-contrast microscope (20X) changes of cell lines towards different concentrations of As-AgNPs, (B) Viability of MCF-7 tumour cell line on exposure to different concentrations of As-AgNPs.

The cytotoxic effect of the As-AgNPs indicates that the biosynthesized AgNPs can be used as chemotherapeutic agents.29 Green synthesized AgNPs reduced the surface attachment owing to reducing agents attached within the inner surface of Ag+ and converted in lower size of molecules within 1–100 nm. It can provide information on cell death, survival, and metabolic activities during cytotoxicity analysis. The cytotoxic effects induced by the As-AgNPs at lower concentrations could be due to the plant components attached to the AgNPs.30 The results obtained from this study confirmed the cytotoxic effect of biosynthesized AgNPs, which has been reported in several studies. For example, the maximum number of cell death was observed in breast cancer MCF-7 cell line with lower concentration by ND-AgNPs.31 Another study also confirmed that the lower concentration exhibited cytotoxicity oral fibroblasts used for A. muricata extract,32 The similar report suggested that the cytotoxicity of AgNPs depends on the dose-dependent manner in MCF-7 cells by S. striata AgNPs.33 HeLa cell line using pomegranate leaf extract, Cannonball leaves AgNPs against MCF-734 and HCT8 used for plant seed extract AgNPs.35 Synthesized AgNPs with a large surface to volume ratio easily enter cells, interact with cell constituents, and interfere with cellular signaling pathways. AgNPs interact with mitochondria and affect cellular chain function. These interactions cause an increase in the ROS level, resulting in oxidative stress, as reported previously.36 The high cytotoxicity of AgNPs against cancerous cells is due to the high uptake of nanoparticles by cancer cells rather than healthy cells. Cancerous cells exhibit abnormal metabolism and a high proliferation rate, which in turn facilitate their vulnerability to AgNPs.3739 Asafoetida is rich in polyphenols and flavonoids, and nanoparticles inspired by this material have rich antioxidant potential.1,2 The current AgNPs with polyphenols are involved in ROS and inhibit the transcription process. It is noteworthy that antioxidants such as polyphenols show cytotoxicity only against non-healthy cells. This behavior supports the observation that the nanoparticles exhibit concentration-dependent toxicity.

The antimicrobial activity of the synthesized silver nanoparticles was examined towards selected bacterial and fungal pathogens, and the zone of inhibition was compared to that obtained by using standard antibiotics. The bacterial pathogens included S. aureus VR83198 (VRE), S. aureus WS10, E. faecium ATCC 43300 (MRSA), E. coli ESBL937316, E. coli ATCC 25922, S. typhi WS03, S. typhimurium ATCC 700603, K. pneumoniae MDR 4414, Salmonella sp. WS50 and A. baumanni MDR 4414 (Table 1, Figure 7A); and the pathogenic yeast included C. albicans ATCC8436, C. krusei ATCC 6258 and C. parapsilosis ATCC22,019 (Table 1, Figure 7B). The zone of inhibition ranged between 7.00 and 15.00 mm. However, the As-AgNPs did not inhibit E. faecium VR 83198, A. baumanni MDR 4414 or C. parapsilosis ATCC 22019. The As-AgNPs significantly inhibited the other microbes (Table 1). The present antimicrobial evaluation studies positively coordinated with reports of Sangeetha et al,40 and Mohammed et al.41

Table 1 Zone of Inhibition in Diameter of As-AgNPs Against Selected Microbial Pathogens

Figure 7 (A) Antimicrobial activity of As-AgNPs against selected bacterial pathogens, (B) Antifungal activity of As-AgNPs against fungal pathogens.

The MIC of the As-AgNPs was 7.80 μg/mL for E. coli ATCC 25,922 and 7.80 μg/mL for S. typhi WS03 and Salmonella sp. WS50, whereas the MIC for S. aureus WS 10 and S. typhimurium ATCC 14,028 was 15.60 μg/mL. Conversely, MICs of 250.00 and 500.00 μg/mL were observed against multidrug-resistant strains, viz., A. baumanni MDR 4414 and E. faecium VR 83198, respectively (Table 2).

Table 2 MIC Value of as-AgNPs for Different Bacterial Pathogens

The mechanism of the antimicrobial activity of As-AgNPs might interact with the microbial membrane, prominently to membrane damage, it will kill the bacteria.42 The As-AgNPs first attachment to the cell membrane and the interruption and release of cellular constituents, resulting in a loss of permeability.43 The antimicrobial activity could also be an interaction between small bacterial cells and AgNPs induce a metabolic imbalance in microbial cells after the assimilation of As-AgNPs, leads to the formation of intracellular reactive oxygen species that kills bacteria consequently.44

The antimicrobial effect of silver nanoparticles is dominated by their morphological characteristics and concentration. Smaller nanoparticles have the advantage of higher percentages of interactions when compared to larger particles. The smallest nanoparticles displayed maximum activity towards the microbial pathogens, despite being more prone to diffusion than larger particles.45 The antimicrobial efficiency of nanoparticles is also based on their shape.46 In particular, triangular nanoparticles have shown more significant inhibitory activity than spherical and rod-shaped AgNPs.47 In addition, the concentration significantly influences the antimicrobial activities of silver nanoparticles. For example, a 50 to 100 mg of total silver content is required to reduce bacterial colony-forming units. The AgNPs were mainly extracellular, and the resulting nanoparticle size range was 5 to 100 nm, specifically 10–50 nm, with spherical shapes.1820 The antimicrobial and antitumor potential of As-AgNPs demonstrates the comprehensive development of new antimicrobial particles with an improved antimicrobial mechanism against human microbial pathogens.

According to the objectives of our current work, the As-AgNPs were synthesized using Asafoetida with significant range and size. Further, the synthesized As-AgNPs were exhibited efficient cytotoxicity and antimicrobial activities against cell lines and microbial pathogens, respectively. These outcomes open a new way to the formulation of As-AgNPs for in vivo studies with animal models in our future research investigations.


This research concludes that As-AgNPs have significant effects on cancer cell lines and microbial pathogens. The simply prepared biologically inspired As-AgNPs were dispersed equally with a small size of 5.6–8.6 nm. The As-AgNPs were potentially active in inhibiting the multiplication of cancer cells in the tested MCF-7 cell lines and exhibited MIC activities with values of 7.80 μg/mL for E. coli and S. typhi, 31.20 μg/mL for K. pneumoniae and S. aureus, and 15.60 μg/mL for S. typhimurium. In addition, the As-AgNPs displayed antifungal efficiency towards C. albicans with an MIC value of 15.60 μg/mL. Overall, the green synthesis of As-AgNPs is an effective way to combat cancer cells and microbial pathogens. This is the first report related to the asafoetida-mediated green synthesis of silver nanoparticles and their biological competence activities.


The authors are grateful to the Deanship of Scientific Research, King Saud University for funding through Vice Deanship of Scientific Research Chairs.

Research Chairs Program King Saud University Riyadh Saudi Arabia Research Chairs Program King Saud University Riyadh Saudi Arabia.


The authors report no conflicts of interest in this work.


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