Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
Received 28 April 2020
Accepted for publication 9 June 2020
Published 19 June 2020 Volume 2020:15 Pages 4351—4362
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Thomas Webster
Sandhanasamy Devanesan,1,2 Karuppiah Ponmurugan,3 Mohamad S AlSalhi,1,2 Naif Abdullah Al-Dhabi3
1Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia; 3Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Correspondence: Mohamad S AlSalhi
Research Chair in Laser Diagnosis of Cancer, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia
Purpose: The present study synthesized silver nanoparticles (AgNPs) using the aqueous extract of a traditional medicinal product consisting of an oleoresin (a combination of macromolecules of carbohydrates and proteins) exuded from the rhizome of the plant Ferula foetida (asafoetida gum) and evaluated its biological properties.
Materials and Methods: The silver nanoparticles synthesized using asafoetida gum (As-AgNPs) were characterized using UV/Vis spectroscopy, fourier infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and transmission electron microscopy (TEM) and EADX. The cytotoxicity and antimicrobial activity As-AgNPs were evaluated against MCF-7 cell lines and selected microbial pathogens, respectively.
Results: The synthesized silver nanoparticles were crystalline in nature with a spherical shape. The average particle size was 5.6– 8.6 nm. The cytotoxicity of the synthesized As-AgNPs was evaluated against MCF-7 cell lines, and the As-AgNPs were found to be effective in inhibiting the multiplication of cancer cells. The As-AgNPs exhibited significant antimicrobial activity towards E. coli, K. pneumoniae and C. albicans. The MIC of the synthesized As-AgNPs was 7.80 μg/mL for E. coli ATCC 25922, Salmonella sp. WS50- and S. typhi; 15.60 μg/mL for S. typhimurium and S. aureus WS10, and 31.20 μg/mL for K. pneumoniae and S. aureus ATCC 43300-MRSA. In addition, MIC values of 15.60 μg/mL for C. albicans ATCC8436 and 31.20 μg/mL for C. krusei ATCC6258 were obtained.
Conclusion: As asafoetida is a good traditional medicine, its involvement in the synthesis of AgNPs led the silver nanoparticles to exhibit good cytotoxic and antimicrobial effects.
Keywords: asafoetida gum, AgNPs, antimicrobial, cytotoxicity, biomedical agent
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