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Cytokines and chemokines expression in serum of patients with neuromyelitis optica

Authors Ai NP, Liu HJ, Zhou HF, Lin DH, Wang JQ, Yang M, Song HG, Sun MM, Xu QG, Wei SH

Received 25 August 2018

Accepted for publication 19 December 2018

Published 21 January 2019 Volume 2019:15 Pages 303—310

DOI https://doi.org/10.2147/NDT.S185336

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Yu-Ping Ning


Nanping Ai,* Hongjuan Liu,* Huanfen Zhou, Dahe Lin, Junqing Wang, Mo Yang, Honglu Song, Mingming Sun, Quangang Xu, Shihui Wei

Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, People’s Republic of China

*These authors contributed equally to this work

Objective: To study the differences in immunopathogenesis based on chemokine profile in neuromyelitis optica patients positive for AQP4 antibodies or MOG antibodies.
Patients and methods: We measured 52 cytokines/chemokines using ELISA in 59 serum samples, which were divided into three groups according to CBA results: HCs (n=16), AQP4+(n=20) and MOG+ (n=23). The regression equation (R2>0.98) of the standard curve was calculated according to the standard concentration and the corresponding A value. And then the corresponding sample concentration was calculated according to the A value of the sample.
Results: Eleven of 52 measured serum cytokine/chemokines (CCL22/MDC, CCL13/MCP-4, CCL21/6Ckine, CCL27/CTACK, CCL8/MCP-2, CXCL14/BRAK, Contactin-1, Kallilrein 6/Neurosin, Midkine, VCAM-1 and Fas) were significantly different between MOG+ group and controls. Ten of 52 measured serum cytokine/chemokines (CCL1/I-309, CCL22/MDC, CCL28, CCL17/TARC, CCL27/CTACK, CXCL2/GRO beta, Contactin-1, Midkine, Chemerin and Synuclein-alpha) were significantly different between AQP4+ group and controls. There was no difference between serum AQP4+ and MOG+ groups for CC chemokines. All measured chemokines CXC except CXCL6/GCP-2 showed no significant differences in serum AQP4+ group compared to MOG+ group. However, there was significant difference between serum AQP4+ and MOG+ groups for C5/C5a and Midkine. C5/C5a and Midkine were significantly higher in AQP4+ group compared to MOG+ group (P<0.05).
Conclusion: Our findings suggest that the differences of mean concentration in CXCL6/GCP-2, Midkine and C5/C5a probably reveal different immunologic mechanism between AQP4+ NMO and MOG+ NMO. This cytokine/chemokine profiling provides new insight into NMO pathogenesis associated with MOG antibody seropositivity and provides guidance to monitor inflammation and response to treatment in a way.

Keywords: NMO, AQP4 antibodies, MOG antibodies, cytokines, chemokines

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